Resurfacing of rodent antibodies

ABSTRACT

The invention relates to a method for providing, via resurfacing, humanized rodent antibodies that have improved therapeutic efficacy due to the presentation of a human surface in the variable region. In the method, (1) position alignments of a pool of antibody heavy and light chain variable regions is generated to give a set of heavy and light chain variable region framework surface exposed positions wherein the alignment positions for all variable regions are at least about 98% identical; (2) a set of heavy and light chain variable region framework surface exposed amino acid residues is defined for a rodent antibody (or fragment thereof); (3) a set of heavy and light chain variable region framework surface exposed amino acid residues that is most closely identical to the set of rodent surface exposed amino acid residues is identified; (4) the set of heavy and light chain variable region framework surface exposed amino acid residues defined in step (2) is substituted with the set of heavy and light chain variable region framework surface exposed amino acid residues identified in step (3), except for those amino acid residues that are within 5 Ångstroms of any atom of any residue of the complementarity determining regions of the rodent antibody; and (5) the humanized rodent antibody having binding specificity is produced.

FIELD OF THE INVENTION

The present invention relates to the development of prediction rules that can be used to accurately model the variable regions (V-regions) of antibodies. The development of these rules and their application in the predictive molecular restructuring of the surfaces of variable domains of non-human monoclonal antibodies enables changing of the surface, i.e., resurfacing, of these monoclonal antibody V-regions to replicate the surface characteristics found on human antibody V-regions. This method of resurfacing non-human monoclonal antibody V-regions to resemble human antibody V-regions is expected to permit the production of functional altered antibodies, which retain the binding parameters of the original non-human monoclonal antibody, with improved therapeutic efficacy in patients due to the presentation of a human surface on the V-region.

BACKGROUND OF THE INVENTION

General Background of Antibodies

Murine monoclonal antibodies are widely used as diagnostic and therapeutic agents in the treatment of human disease. Mice can be readily immunized with foreign antigens to produce a broad spectrum of high affinity antibodies. Invariably, the introduction of murine or other rodent antibodies into humans results in the production of a human anti-mouse antibody (HAMA) response due to the presentation of a foreign protein in the body. The production of HAMA in patients can result from the introduction of foreign antibody in a single dose or from extended use in therapy, for example, for the treatment of cancer. Extended use of murine antibody is generally limited to a term of days or weeks in patients before concerns of anaphylaxis arise. Moreover, once HAMA has developed in a patient, future use of murine antibodies for diagnostic or therapeutic purposes is often precluded for the same reasons.

Beyond ethical considerations, attempts to produce human monoclonal antibodies have not been highly successful for a number of reasons. The production in vitro of human monoclonals rarely results in high affinity antibodies. In vitro cultures of human lymphocytes yield a restricted range of antibody responses relative to the broad spectrum of reactive antibodies produced in vivo through direct immunization of mice. Additionally, in humans, immune tolerance prevents the successful generation of antibodies to self-antigens. All of these factors have contributed to the search for ways to modify the structures of murine monoclonal antibodies to improve their use in patients. Many investigators have attempted to alter, reshape or humanize murine monoclonal antibodies in an effort to improve the therapeutic application of these molecules in patients.

Strategies of Antibody Humanization

The earliest reports of the controlled rearrangement of antibody domains to create novel proteins was demonstrated using rabbit and human antibodies as described by Bobrzecka, K. et al. (Bobrzecka, K., Konieczny, L., Laidler, P. and Rybarska, J. (1980), Immunology Letters 2, pp. 151-155) and by Konieczny et al. (Konieczny, L., Bobrzecka, K., Laidler, P. and Rybarska, J. (1981), Haematologia 14 (I), pp. 95-99). In those reports, the protein subunits of antibodies, rabbit Fab fragments and human Fc fragments, were joined through protein disulfide bonds to form new, artificial protein molecules or chimetic antibodies.

Recombinant DNA technology was used to construct gene fusions between DNA sequences encoding mouse antibody variable light and heavy chain domains and human antibody light chain (LC) and heavy chain (HC) constant domains to permit expression of the first recombinant "near-human" antibody (chimeric antibody) product (Morrison, S. L., Johnson, M. J., Herzenberg, L. A. and Oi, V. T. (1984), Proc. Natl. Acad. Sci. U.S.A. 81, pp. 6851-6855).

The kinetics and immune response in man to chimeric antibodies has been examined (LoBuglio, A. F., Wheeler, R. H., Trang, J., Haynes, A., Rogers, K., Harvey, E. B., Sun, L., Ghrayeb, J. and Khazaeli, M. B. (1989), Proc. Natl. Acad. Sci. 86, pp. 4220-4224).

Chimeric antibodies contain a large number of non-human amino acid sequences and are immunogenic in man. The result is the production of human anti-chimera antibodies (HACA) in patients. HACA is directed against the murine V-region and can also be directed against the novel V-region/C-region (constant region) junctions present in recombinant chimeric antibodies.

To overcome some of the limitations presented by the immunogenicity of chimeric antibodies, the DNA sequences encoding the antigen binding portions or complementarity determining regions (CDR's) of murine monoclonal antibodies have been grafted by molecular means in the DNA sequences encoding the frameworks of human antibody heavy and light chains (Jones, P. T., Dear, P. H., Foote, J., Neuberger, M. S. and Winter, G. (1986), Nature 321, pp. 522-525; Riechmann, L., Clark, M., Waldmann, H. and Winter, G. (1988), Nature 332, pp. 323-327). The expressed recombinant products called reshaped or humanized antibodies are comprised of the framework of a human antibody light or heavy chain and the antigen recognition portions, CDR's, of a murine monoclonal antibody. Several patent applications have been filed in this area including, for example, European Patent Application, Publication No. 0239400; European Patent Application, Publication Nos. 0438310A1 and 0438310A2; International Patent Publication No. WO 91/09967; and International Patent Publication No. WO 90/07861.

However, it is questionable whether European Patent Application (EP), Publication No. 0239400 is truly enabling. It is not assured in this patent that the best fit is made to assure proper presentation of the CDR loops at the antibody combining site.

EP Publication Nos. 0438310A1 and 0438310A2 go a step beyond EP Publication No. 0239400 by protecting the importance of uniquely selected human frameworks for the human light chain (LC) and heavy chain (HC) V-regions. These V-region frameworks should show a high degree of sequence similarity with the frameworks of the murine monoclonal antibody and present the CDR's in the appropriate configuration. However, the criteria for sequence matching are no more sophisticated than simple homology searching of the antibody protein or DNA databases.

International Patent Publication No. WO 91/09967 attempts a further variation of the method disclosed in EP Publication No. 0239400. In International Patent Publication No. WO 91/09967, homology of the donor sequences and the acceptor framework is not important, rather it discloses that a selected set of residues in the LC and HC are critically important to humanization. The ability to make changes at these positions is the basis of International Patent Publication No. WO 91/09967.

International Patent Publication No. WO 90/07861 proposes four important criteria for designing humanized antibodies. 1) Homology between human acceptor and non-human donor sequences. 2) Use donor rather than acceptor amino acids where the acceptor amino acid is unusual at that position. 3) Use donor framework amino acids at positions adjacent to the CDR. 4) Use donor amino acids at framework positions where the sidechain atom is within 3 Angstroms of the CDR in a 3-D model. The first antibody humanized by this method retained less than 1/3 the affinity of the original monoclonal antibody.

None of the above methods for designing a humanized antibody is predictable due to the questions that surround CDR framework interactions. By replacement of murine framework with human framework, there is no guarantee of identical conformations for CDR's because i) the V_(L) -V_(H) interaction is not identical in all V-regions and ii) accurate prediction of the CDR-framework interactions are key to faithful reproduction of the antigen binding contacts.

The above methods do not offer a general solution to solving the issues surrounding antibody humanization, rather the methods as outlined in each reference above involve a substantial amount of trial and error searching to obtain the desired affinity in the final humanized product. More importantly, there is no guarantee that corrective changes in framework amino acids will leave the reshaped V-regions resembling the surface character of a truly human antibody. Therefore, it can be argued that antibodies humanized by the above methods may be immunogenic in man.

Antigenicity of Antibodies

The antigenicity/immunogenicity of an antibody, including recombinant reshaped antibody products, introduced into humans can be viewed as a surface phenomenon. In general one can view the immune system as scanning the surface of a protein introduced to the body. If the F_(V) portion of a humanized antibody `opens-up` in the circulation then internal residues can be presented to the immune system. On the other hand, if the F_(V) portion is stable and tightly packed then only the surface residues presented by the V-regions and the interface between the V_(L) and V_(H) regions will be `scanned`.

Surface Reshaping or Resurfacing of Antibodies

The notion of surface presentation of proteins to the immune system raises the prospect of redesigning murine monoclonal antibodies to resemble human antibodies by humanizing only those amino acids that are accessible at the surface of the V-regions of the recombinant F_(V). The resurfacing of murine monoclonal antibodies to reduce their immunogenicity could be beneficial in maintaining the avidity of the original monoclonal antibody in the reshaped version, because the natural framework-CDR interactions are retained. The value of maintaining the integrity of the framework-CDR interactions has been illustrated as summarized below.

In a recent research report, two different reshaped versions of the rat monoclonal antibody, Campath-9 (anti-human CD4), were generated (Gorman, S. D., Clark, M. R., Routledge, E. G., Cobbold, S. P. and Waldmann, H. (1991), Proc. Natl. Acad. Sci. U.S.A. 88, pp. 4181-4185). In one version, pV_(H) NEW/C_(G1), the acceptor V_(H) framework was from the human NEW-based heavy chain, which has 47% identical residues to the Campath-9 V_(H). While in the second version, pV_(H) KOL/C_(G1), the acceptor V_(H) framework was from the human KOL antibody, which has 72% identical residues to Campath-9 V_(H). Each reshaped antibody contained the identical V_(L) domain from the human REI antibody sequence. However, the recombinant product of pV_(H) KOL/C_(G1) had an avidity for CD4 that was substantially greater than the product of pV_(H) NEW/C_(G1). The authors proposed a reshaping strategy where human sequences, that are highly homologous to the rodent antibody of interest, are transferred, by in vitro mutagenesis, into the rodent V-region to create a "bestfit" reshaped antibody. This strategy uses the term "bestfit" to describe the modeling process, however, there is no quantitative formula employed to assess "bestfit", and so in effect, the process is subjective. Additionally, there is no resurfacing concept presented in that paper.

The concept of reducing rodent-derived antibody immunogenicity through the replacement of exposed residues in the antibody framework regions which differ from those of human origin is discussed in a recent paper (Padlan, E. A. (1991), Molecular Immunology 28, pp. 489-498). In that paper, the variable domains of two antibody structures, KOL (human) and J539 (mouse), are examined. The crystal structures of the Fab fragments of these two antibodies have been elucidated to high resolution. The solvent accessibility of the exposed framework residues in the variable domains of these two antibodies were compared to a sequence database of human and murine antibody V-region subgroups. On the basis of his findings, Padlan proposed to reduce the antigenicity of allogeneic variable domains [murine V-regions], through replacement of the exposed residues in the framework regions with residues usually found in human antibodies. In murine sequences with the highest similarity to a given human sequence, the number of changes necessary to "humanize" a murine V-region surface would range from 6-15 amino acid changes per V-region. This reference suggests how to convert one antibody surface into another but no general method is developed. Application of the procedure is provided by two examples, a worst-case and a best-case.

Worst Case:

Among the representative murine kappa V_(L) sequences examined for which its autologous V_(H) has been sequenced, S107V_(L) has the most residues that need to be replaced to humanize it. S107V_(L) is most similar to the members of the human subgroup VKIV and JK2. The exposed or partially exposed residues that need to be replaced are those at positions 9, 10, 14, 15, 16, 17, 18, 22, 41, 63, 80, 85, 85, 100 and 106. Murine V-region S107V_(H) is most similar in its framework to the members of the human subgroup VHIII and JH6. The exposed or partially exposed residues in S107V_(H) that need to be replaced are those at positions 3, 40, 68, 73, 75, 76, 82b and 89. A total of 23 residues need to be replaced to humanize the variable domains of S107.

Best Case:

Among the murine V_(H) sequences examined for which the autologous V_(L) has also been sequenced, MOPC21V_(H) has the least number of residues that need to be replaced to humanize it. MOPC21V_(H) is most similar in its framework to the members of the human subgroup HIII and JH6. The exposed or partially exposed residues that need to be replaced are those at positions 1, 42, 74, 82a, 84, 89 and 108. MOPC21V_(L) is most similar in its framework to human subgroup VKIV and JK4. The exposed or partially exposed residues that need to be replaced are those at positions 1, 9, 12, 15, 22, 41, 63, 68, 83 and 85. A total of 17 amino acids need to be replaced to humanize the variable domains of MOPC21.

Of the light chains in the Best- and Worst-Case examples cited above, S107V_(L) required changes at 15 positions and MOPC21V_(L) required changes at 10 positions. Only seven of the changes are common to both of these light chain sequences (see underlined residues). Moreover, of the heavy chain residues that need to be replaced to humanize the respective V-regions, S107V_(H) required changes at 8 positions and MOPC21V_(H) required changes at 7 positions. In this instance, only one position is common to both of these heavy chain sequences (see residues in boldface).

An analysis of S107 V-regions alone would not have led to the prediction of which residues to change in MOPC21. The reason for this is that the surface residues in Padlan's analysis are only determined by reference to the crystal structure analysis of one antibody. In addition, the basis for defining the surface exposure of an amino acid at a particular position on that crystal structure is a continuous gradient of change, e.g., the fractional solvent accessibility values (Padlan, E. A. (1990), Molecular Immunology 28, pp. 489-498) were computed, where: 0 to 0.2=completely buried, 0.2 to 0.4=mostly buried, 0.4 to 0.6=partly buried/partly exposed, 0.6 to 0.8=mostly exposed, and 0.8 or above=completely exposed. By limiting the analysis of exposed surface residues to a single crystal structure and by superimposing a broad range of solvent accessibility ratios on exposed residues, such a modeling strategy could be expected to have a wide margin of error in its calculations. This model fails to take into account the great majority of structural information available in the database for other antibody crystal structures.

SUMMARY OF THE INVENTION

Accordingly, it is an object of this invention to provide humanized rodent antibodies or fragments thereof, and in particular, humanized rodent monoclonal antibodies that have impinged therapeutic efficacy in patients due to the presentation of a human surface on the V-region. This and other objects have been attained by providing a method for determining how to humanize a rodent antibody or fragment thereof by resurfacing the method comprising:

(a) determining the conformational structure of the variable region of the rodent antibody or fragment thereof by constructing a three-dimensional model of the rodent antibody variable region;

(b) generating sequence alignments from relative accessibility distributions from x-ray crystallographic structures of a sufficient number of rodent and human antibody variable region heavy and light chains to give a set of heavy and light chain framework positions wherein the alignment positions are identical in 98% of the sufficient number of rodent antibody heavy and light chains;

(c) defining for the rodent antibody or fragment thereof to be humanized a set of heavy and light chain surface exposed amino acid residues using the set of framework positions generated in step (b);

(d) identifying from human antibody amino acid sequences a set of heavy and light chain surface exposed amino acid residues that is most closely identical to the set of surface exposed amino acid residues defined in step (c), wherein the heavy and light chain from the human antibody are or are not naturally paired;

(e) substituting, in the amino acid sequence of the rodent antibody or fragment thereof to be humanized the set of heavy and light chain surface exposed amino acid residues defined in step (c) with the set of heavy and light chain surface exposed amino acid residues identified in step (d);

(f) constructing a three-dimensional model of the variable region of the rodent antibody or fragment thereof resulting from the substituting specified in step (e);

(g) identifying, by comparing the three-dimensional models constructed in steps (a) and (f), any amino acid residues from the sets identified in steps (c) or (d), that are within 5 Angstroms of any atom of any residue of the complementarity determining regions of the rodent antibody or fragment thereof to be humanized; and

(h) changing any residues identified in step (g) from the human to the original rodent amino acid residue to thereby define a rodent antibody humanizing set of surface exposed amino acid residues; with the proviso that step (a) need not be conducted first, but must be conducted prior to step (g).

Also provided is a method for producing a humanized rodent antibody or fragment thereof from a rodent antibody or fragment thereof, the method comprising:

(I) carrying out the above-described method for determining how to humanize a rodent antibody or fragment thereof by resurfacing; and

(II) modifying the rodent antibody or fragment thereof by replacing the set of rodent antibody surface exposed amino acid residues with the rodent antibody humanizing set of surface exposed amino acid residues defined in step (h) of the above-described method.

In a preferred embodiment, the rodent antibody or fragment thereof is a murine antibody, and most preferably murine antibody N901.

BRIEF DESCRIPTION OF THE FIGURES

FIG. 1 shows an algorithm that can be used for constructing a three-dimensional model of the rodent antibody variable region.

FIG. 2 is a diagram showing the approach to determine how to humanize a rodent antibody or fragment thereof according to the present invention.

FIGS. 3A SEQ ID NOS: 1-12 and 3B SEQ ID NOS: 13-24 are plots of relative accessibility of amino acid residues for twelve antibody F_(V) structures, mapped onto the sequence alignment of these structures. Structures Glb2 (Jeffrey, P. D., Doctor of Philosophy Thesis, University of Oxford, United Kingdom, 1991), D1.3 (Amit, A. G., Mariuzza, R. A., Phillips, S. E. V. and Poljak, R. J. (1986), Science 233, pp. 747-753), 3D6 (Grunow, R., Jahn, S., Porstman, T., Kiessig, T., Steinkeller, H., Steindl, F., Mattanovich, D., Gurtler, L., Deinhardt, F., Katinger, H. and von R., B. (1988), J. Immunol. Meth. 106, pp. 257-265) and 36-71 (5fab) (Rose, D. R., Strong, R. K., Margolis, M. N., Gefter, M. L. and Petsko, G. A. (1990), Proc. Natl. Acad. Sci. U.S.A. 87, pp. 338-342) are not yet present in the Brookhaven database. The other structures used were: 2hfl (Sheriff, S., Silverton, E. W., Padlan, E. A., Cohen, G. H., Smith-Gill, S. J., Finzel, B. C. and Davies, D. R. (1987), Proc. Natl. Acad. Sci. U.S.A. 84, pp. 8075-8079), 3hfm (Padlan, E., Silverton, E., Sheriff, S., Cohen, G., Smith-Gill, S. and Davies, D. (1989), Proc. Natl. Acad. Sci. U.S.A. 86, pp. 5938-5942), 2fbj (Mainhart, C. R., Potter, M. and Feldmann, R. J. (1984), Mol. Immunol. 21, pp. 469-478), 3fab (Saul, F. A., Amzel, L. M. and Poljak, R. J. (1978), J. Biol. Chem. 253, pp. 585-597), 4fab (Herron, J., He, X., Mason, M., Voss, E. and Edmunson, A. (1989), Proteins: Struct., Funct., Genet. 5, pp. 271-280), 2mcp (Segal, D., Padlan, E., Cohen, G., Rudikoff, S., Potter, M. and Davies, D. (1974), Proc. Natl. Acad. Sci. U.S.A. 71, pp. 4298), 2fb4 (Marquart, M. Deisenhofer, J. and Huber, R. (1980), J. Mol. Biol. 141, pp. 369-391), and 1f19 (Lascombe, M. Alzari, P., Boulot, G., Salujian, P., Tougard, P., Berek, C., Haba, S., Rosen, E., Nisonof, A. and Poljak, R. (1989), Proc. Natl. Acad. Sci. U.S.A. 86, p. 607). These structures are designated by their Brookhaven entry code. The sequence numbering used here is described in FIGS. 4A and 4B. FIG. 3A graphically shows the relative accessibility for the heavy chain and FIG. 3B graphically shows the relative accessibility for the light chain.

FIGS. 4A SEQ ID NOS: 25-31 and 4B SEQ ID NOS: 32-38 show alignments of sequences generated using the three methods of humanization. Sequences are: 1) Original rodent N901. 2+3) KOL (Marquart, M. Deisenhofer, J. and Huber, R. (1980), J. Mol. Biol. 141, pp. 369-391) and reshaped N901 using KOL surface. 4+5) Most homologous sequences, L(KV2F) (Klobeck, H., Meindl, A., Combriato, G., Solomon, A. and Zachau, H. (1985), Nucleic Acids Res. pp. 6499-6513) and H(G36005) (Schroeder, H. and Wang, J. (1990), Proc. Natl. Acad. Sci. U.S.A. 87), and reshaped N901 using these sequences. 6+7) Most homologous with respect to surface residues, L(KV4B) (Klobeck, H., Bronkamp, G., Combriato, G., Mocikat, R., Pohelnz, H. and Zachau, H. (1985), Nucleic Acids Res. 3, pp. 6515-6529) and H(PLO123) (Bird, J., Galili, N., Link, M., Sites, D. and Sklar, J. (1988), J. Exp. Med. 168, pp. 229-245), and reshaped N901 using these sequences. The numbering is the same as used in the antibody modelling program ABM (trademark for commercial software, Oxford Molecular Ltd., Oxford, U.K.), which is based on structural conservation and not sequence homology as used by Padlan et al. (Kabat, E. A., Wu, T. T., Reid-Miller, M., Perry, H. M. and Gottesman, K. S. (1987), Sequences of Proteins of Immunological Interest. U.S. Department of Health and Human Services, Fourth Edition). The sequence changes which have to be introduced in order to resurface N901 with a given sequence are marked with bars, back-mutations as determined from F_(V) models are marked with stars. The sequence homology of given sequences to N901 are shown in brackets after each sequence.

FIG. 5 is a stereo plot of mean antibody β-barrel, coordinates determined by iterative multiple fitting of eight antibody structures. Strands 7 and 8 comprise the `take off` positions for CDR H3 and are not included in the fitting of V_(L) and V_(H) regions.

FIG. 6 is a plot of RMS deviation (RMSD) from the mean of the eight β-sheet strands comprising the framework. The RMSD was calculated from structures F19.9, 4-4-20, NEW, FBJ, KOL, HyHEL-5, HyHEL-10 and McPC603. N,Cα,C atoms are included in the plot. The residues used are shown in the alignment (Table 2). The most disordered residues are all the residues of strand HFR4, the last residue of LFR1, and the first and last residue of HFR2. The nomenclature of the strands is explained in the alignment in Table 2. LFR1-#1, LFR2-#2, LFR3-#3, LFR4-#4, HFR1-#5, HFR2-#6, HFR3-#7, HFRS4-#8.

FIG. 7 is a flowchart of the overall modelling protocol known as CAMAL.

FIG. 8 is a plot of superimposed loop backbones for models and x-ray structures discussed in Example 2. The loops are positioned after global framework fit. This does not represent the best local least squares fit, but shows how the loops are positioned globally onto the framework.

FIGS. 9A to 9D are stereo (N,C-α,C,O) representations of crystal structures and models of D1.3, 3671 and Gloop-2 variable domain and β-barrel strands described in Example 2. Crystal structures are shown with open bonds, model with solid bonds. The difference between the 3D6-H3 in the model and the crystal structure is due to a 5°-7° twist in the extended β-sheet conformation of this loop, FIG. 9A: D1.3, FIG. 9B: 36-71, FIG. 9C: Gloop-2, FIG. 9D: 3D6.

FIG. 10 is a histogram showing the distribution of loop length for CDR H3 loops, data from Kabat et al. (Kabat, E. A., Wu, T. T., Reid-Miller, M., Perry, H. M. and Gottesman, K. S. (1987), Sequences of Proteins of Immunological Interest. U.S. Department of Health and Human Services, Fourth Edition).

DETAILED DESCRIPTION OF THE INVENTION

The existence of specific, yet different, surface patches in murine and human antibodies may be the origin of the inherited immunogenicity of murine antibodies in humans. Statistical analysis of a database of unique human and murine antibody F_(V) fragments has revealed that certain combinations of residues in exposed surface positions are specific for human and murine sequences. The combinations are not the same in human and murine F_(V) domains. However, it is possible to define families of surface residues for the two species of antibodies. These families reveal a novel method for the "humanization" or reshaping of murine antibodies. Humanization is the modification of the solvent accessible surface of a non-human antibody or fragment thereof to resemble the surface of a chosen human antibody or fragment thereof such that the modified non-human antibody or fragment thereof exhibits lower immunogenicity when administered to humans. Such a process applies in the present application to antibody variable regions but could equally well apply to any other antibody fragment. The method is considered to be generally applicable to humanization of rodent antibodies.

According to the present invention, a statistical analysis is presented which is based on accessibility calculated for a range of antibody crystal structures. When this information is applied to an antibody sequence database, it is possible to discriminate between human and murine antibodies at the sequence level purely on the basis of their surface residue profiles.

Rational Resurfacing Approach

There are several key features of the resurfacing approach of the present invention.

1) This method uses as a starting point, construction of a three-dimensional model of a rodent variable region by known methods;

2) A large number (e.g., twelve) of antibody F_(V) or Fab fragment x-ray crystallographic structures are analyzed to produce an unambiguous set of surface exposed amino acid residues that will be positionally identical (about 98%) for a majority of antibodies. The set is produced by identifying all those residues whose solvent accessibility is above a given cut-off (typically 30%), calculated using a modification of the method of Kabsch and Sander (Kabsch, W. and Sander, C. (1983), Biopolymers 22, pp. 2257-2637) in which explicit atomic radii are used for each atom type to predict sidechain positions as is described below in more detail;

3) Using a complete human antibody database, the best set of human heavy and light chain surface exposed amino acid residues is selected on the basis of their closest identity to the set of surface amino acid residues of the murine antibody;

4) In order to retain the conformational structure of the CDRs of the rodent antibody, replacement of any human surface exposed amino acid with the original rodent surface exposed amino acid residue is carried out whenever a surface residue in either the rodent or resurfaced antibody is calculated from the three-dimensional model to be within 5 Angstroms of a CDR residue.

The general resurfacing approach of the present invention is illustrated in FIG. 2. The approach can be divided into two stages. In the first, the rodent framework (white) is retained and only the surface residues changed from rodent (dark grey circles) to the closest human pattern (light grey circles). This should remove the antigenicity of the rodent antibody. In the second stage, surface residues within 5 Angstroms of the CDRs are replaced with the rodent equivalents in an attempt to retain antigen binding and CDR conformation.

The method of the present invention is applicable to whole antibodies as well as antibody fragments. Suitable antibody fragments that can be used can readily be determined by the skilled artisan. Examples of some suitable fragments include a single chain antibody (SCA), an antibody F_(V) fragment, Fab fragment, F(ab')₂ fragment, Fab' fragment, or other portion of an antibody comprising the binding site thereof.

According to the present invention, an important step in the method for determining how to modify a rodent antibody or fragment thereof by resurfacing is to determine the conformational structure of the variable region of the rodent antibody or fragment thereof to be humanized by constructing a three-dimensional model of the rodent antibody variable region. This can be done by known methods such as those described, for example, in Martin et al. (Martin, A. C. R., Cheetham, J. C. and Rees, A. R. (1989), Proc. Natl. Acad. Sci. U.S.A. 86, pp. 9268-9272; Methods in Enzymology (1991), 203, pp. 121-152) and as described in detail in Example 2.

Martin et al. describe an algorithm which is depicted in FIG. 1. The algorithm applies to murine and human antibodies equally well. The present inventors therefore expect that, based on sequence similarity between antibodies of different species (Kabat, E. A. Segments of Proteins of Immunological Interest, National Institutes of Health, U.S.A. 1991), the algorithm will work equally well for rat and other rodent antibodies.

Briefly, the algorithm depicted in FIG. 1 can be summarized as follows. The framework region of an antibody to be modelled is selected on the basis of sequence homology and constructed by a least squares fit onto the six conserved strands of the variable region β-barrel. Light and heavy chain complementarity determining regions are constructed using a combination of canonical structures (Chothia, C. and Lesk, A. M. (1987), J. Molec. Bio. 196, pp. 901-917), database searching and conformational searching. Detailed descriptions of these methods are described in Example 2 herein and in the above two references (Martin et al. 1989 and 1991).

According to the present invention, another three-dimensional model is also constructed. The other three-dimensional model is of the rodent antibody variable region having human antibody surface amino acid residues substituted therein at particular rodent antibody surface residue positions.

This other three-dimensional model is constructed by carrying out the series of steps described next.

The first of the steps is to generate sequence alignments from relative accessibility distributions from x-ray crystallographic structures of a sufficient number of rodent antibody variable region heavy and light chains to give a set of framework positions of surface exposed amino acid residues the positions of which are 98% identical in a majority of the variable regions.

As used herein, the term "framework" means the antibody variable region from which the complementarity determining regions have been excluded.

"Complementarity determining regions" means those amino acid sequences corresponding to the following numbering system as defined by Kabat, E. A. (In Sequences of Immunological Interest, N.I.H., U.S.A., 1991).

    ______________________________________                                         Light Chain   L1      residues    24-34                                        Light Chain   L2      residues    50-56                                        Light Chain   L3      residues    89-97                                        Heavy Chain   H1      residues    31-358                                       Heavy Chain   H2      residues    50-58                                        Heavy Chain   H3      residues    95-102                                       ______________________________________                                    

A sufficient number of rodent antibody fragments that need to be analyzed in order to produce the set of framework positions of surface exposed amino acid residues can readily be determined by the skilled artisan through routine experimentation using a database of antibody sequences. Thus, this step can be conducted using suitable databases now in existence or later compiled.

The x-ray crystallographic structures are used to determine relative accessibility distributions of surface exposed amino acid residues. The relative accessibility distributions identify all those residues whose solvent accessibility is above a given cut-off (typically 30%), calculated using a modification of the method of Kabsch and Sander (Kabsch, W. and Sander C. (1983), Biopolymers 22, pp. 2257-2637) in which explicit atomic radii are used for each atom type.

The relative accessibility distributions determined from the x-ray crystallographic structures can then be used to generate sequence alignments which give a set of framework positions of surface exposed amino acid residues the positions of which are 98% identical in a majority of the variable regions.

The set of framework positions of surface exposed amino acid residues for the variable regions of murine antibodies is shown in Table 1, set forth in Example 1, and was produced using the sequence alignments and accessibility distributions shown in FIGS. 3A and 3B.

Once a set of framework positions of surface exposed amino acid residues for the variable regions of the rodent antibodies have been generated, the surface exposed residues of the heavy and light chain pair of the rodent antibody, or fragment thereof, to be humanized can be identified using an alignment procedure such as that described in Example 1 and shown in FIGS. 3A and 3B. This defines a set of surface exposed amino acid residues of a heavy and light chain pair of a rodent antibody or antibody fragment to be humanized.

Next, a complete human antibody sequence database is used to identify a set of surface exposed amino acid residues from a human antibody variable region that have the closest positional identity to the set of surface exposed amino acid residues of the variable region of the rodent antibody that is to be humanized. The set of surface exposed amino acid residues from the human antibodies can be separately identified for a heavy chain and for a light chain that are not naturally paired and/or a set can be identified from a natural human heavy and light chain pair, that is, a pair originating from a single B cell or hybridoma clone. Preferably, the set is one from a natural human heavy and light chain pair.

A humanized rodent antibody that gives the appearance of a human antibody is then predicted by substituting the set of surface exposed amino acid residues from the rodent antibody or fragment thereof to be humanized with the set of surface exposed amino acid residues from the human antibody.

A three-dimensional model can then be constructed from the resulting, fully substituted variable region of the rodent antibody or fragment thereof. The three-dimensional model is constructed using the same known methods mentioned above for constructing a 3-D model of the original rodent antibody or fragment thereof.

While the antigenicity of this fully "resurfaced" or humanized antibody should be removed, an additional factor to be addressed is the binding affinity or the binding strength of the resurfaced antibody. Changes in the framework of the variable domain introduced through resurfacing can influence the conformation of the CDR loops and therefore antigen binding of the antibody. According to the present invention, this problem is removed by the next step which is to identify, by means of a comparison of both of the above-described three-dimensional models of the rodent antibody variable region, any residues from the set of surface exposed amino acid residues of the variable region heavy and light chain pair of the rodent or human antibody identified that are within 5 Angstroms of any atom of any residue of the rodent antibody or antibody fragment complementarity determining regions (CDRs).

Any residue(s) so identified is then changed back from the human to the original rodent amino acid residue(s).

The results of this method can then be applied to a particular rodent antibody by well known methods. Briefly, genes for the humanized variable heavy and light chain regions are constructed using standard recombinant DNA methods (Sambrook, J., Fritsch, E. F. and Maniatis, T. (1989), Molecular Cloning, Second Edition). For example, a PCR method can be used (Daugherty et al. (1991), Nucleic Acids Research 19, pp. 2471-2476).

Variable heavy chain or variable light chain gene constructs are subcloned into appropriate expression vectors. Suitable expression vectors contain either a human gamma or human kappa constant region gene, a suitable promoter, a sequence coding for a human immunoglobulin leader peptide (for example: met-gly-trp-ser-cys-ile-ile-leu-phe-leu-val-ala-thr-ala-thr (SEQ ID NO:39), Olandi et al. (1989), PNAS 86, pp. 3833-3837), and a drug selectable marker.

Heavy and light chain expression plasmids can be co-transfected, for example, by electroporation into suitable cells, for example, SP2/0 cells, and selected with an appropriate drug, G418, for example. Screening for intact antibody can be accomplished by ELISA assay. 96-well plates are coated with, for example, goat anti-human kappa chain antibody, and light chains are detected with, for example, goat anti-human antibody conjugated to alkaline phosphatase.

As another approach, light chain constructs are transfected, for example, by electroporation into suitable cells, for example, SP2/0 cells and selected, for example, in hygromycin. Screening for light chain expression can be accomplished by ELISA assay. 96-well plates are coated with, for example, goat anti-human kappa chain antibody, and light chains are detected with, for example, goat anti-human antibody conjugated to alkaline phosphatase.

A light chain producing line is then used as a host to electroporate in the heavy chain construct. The heavy chain plasmid is co-transfected with a plasmid containing the gene coding for another drug marker, for example, neomycin resistance and selected in the presence of the drug G418. Screening for intact antibody is accomplished by ELISA assay. 96-well plates are coated with, for example, goat anti-human Fc and detected with, for example, goat anti-human light chain conjugated to alkaline phosphatase.

EXAMPLE 1 AND COMPARATIVE EXAMPLES

The superiority of the presently claimed method for determining how to modify a rodent antibody or fragment thereof by resurfacing in order to produce a humanized rodent antibody will now be described by reference to the following example and comparative examples which are illustrative and are not meant to limit the present invention.

A) Analysis for Murine Antibodies

In order to determine the positions which are usually accessible on the surface of the F_(V) domain of murine antibodies, the accessibility was calculated for twelve Fab x-ray crystallographic structures obtained from the Brookhaven database (Bernstein, F., Koetzle, T., Williams, G., Meyer, E., Brice, M., Rodgers, J., Kennard, O., Shimanouchi, T. and Tasumi, M. (1977), J. Mol. Biol. 112, pp. 535-542). The relative accessibility was calculated using the program MC (Pedersen, J. (1991)), which implements a modified version of the DSSP (Kabsch, W. and Sander, C. (1983), Biopolymers 22, pp. 2257-2637) accessibility calculation routine in which explicit atomic radii are specified for every atom. A residue was defined as being surface accessible when the relative accessibility was greater than 30%. The alignment positions of these residues were conserved in all twelve structures (98% identity). Surface accessible framework positions constitute 40% of the F_(V) surface area. The remaining surface accessible residues are in the CDRs and in the interdomain C-terminal region. FIGS. 3A and 3B show a sequence alignment of the twelve crystal structures, the average relative accessibility, and the 30% accessibility cutoff. FIG. 3A shows the alignments and relative accessibility for the twelve murine antibody light chains and FIG. 3B shows the alignments and relative accessibility for the murine antibody heavy chains.

The surface accessible framework positions were mapped onto a database of unique human and mouse F_(V) sequences (see lists at the end of this Example). The frequency of particular residues in each of these positions is shown in Table 1. Only residue frequencies higher than 5% are listed.

                  TABLE 1                                                          ______________________________________                                         Distribution of accessible residues in human V.sub.H and V.sub.L chain         se-                                                                            quences. All of the positions appear to be conserved, which leads              to the hyphothesis that immunogenecity arises from a specific                  combination of these surface residues. The sequence numbering                  is explained in FIGS. 3A and 3B.                                               Posi-                                                                          tion  Human   Mouse                                                            ______________________________________                                         Light chain                                                                    1     D 51 E 34 A 5 S 5 D 76 Q 9 E 6                                           3     V 38 Q 24 S 24 Y 6                                                                               V 63 Q 22 L 5                                          5     T 61 L 37         T 87                                                   9     9 26 S 26 G 17 A 14 L 7                                                                          S 36 A 29 L 17 P 5                                     15    P 62 V 25 L 12    L 47 P 30 V 8 A 7                                      18    R 57 S 18 T 13 P 6                                                                               R 38 K 22 S 13 Q 12 T 9                                46    P 94              P 82 S 9                                               47    G 89              G 71 D 18                                              51    K 43 R 31         K 70 Q 13 R 8 T 5                                      63    G 91              G 98                                                   66    D 43 S 25 A 9     D 38 A 26 S 26                                         73    S 96              S 90 I 5                                               86    P 44 A 27 S 17 T 8                                                                               A 50 P 11 T 8 E 7 Q 6                                  87    E 71 D 11 G 7     E 91 D 6                                               111   K 74 R 12 N 6     K 93                                                   115   K 54 L 40         K 87 L 5                                               116   R 60 G 33 S 5     R 89 G 9                                               117   Q 50 T 37 E 6 P 6 A 74 Q 14 P 5 R 5                                      Heavy chain                                                                    118   E 47 Q 46         E 59 Q 29 D 10                                         120   Q 83 T 7          Q 68 K 26                                              122   V 59 L 15 Q 13    Q 57 V 27 L 5 K 5                                      126   G 54 A 23 P 18    G 36 9 30 A 29                                         127   G 53 E 22 A 14 D 7                                                                               E 45 G 43 S 6                                          128   L 61 V 31 F 7     L 96                                                   130   K 46 Q 41 E 5     K 52 Q 27 R 17                                         131   P 95              P 91 A 5                                               132   G 74 S 16 T 7     G 82 S 17                                              136   R 53 K 23 S 17 T 7                                                                               K 66 S 17 R 13                                         143   G 96              G 98                                                   145   T 46 S 32 N 9 I 7 T 63 S 19 N 7 A 5 D 5                                  160   P 84 S 10         P 89 H 7                                               161   G 93              G 71 E 24                                              162   K 76 Q 10 R 8     K 50 Q 30 N 10 H 5                                     183   D 26 P 25 A 17 Q 10 T 7                                                                          E 31 P 22 D 17 A 12 Q 11                               184   S 70 K 9 P 8      K 42 S 37 T 6                                          186   K 53 Q 22 R 7 N 5 K 83 Q 7                                               187   G 66 S 21 T 5     G 62 S 18 D 10                                         195   T 30 D 26 N 19 K 7                                                                               T 36 K 30 N 26 D 6                                     196   S 91              S 76 A 16                                              197   K 65 I 8 T 8 R 5  S 46 K 34 Q 11                                         208   R 46 T 18 K 17 D 6                                                                               T 55 R 26 K 8                                          209   A 50 P 21 S 13 T 8                                                                               S 67 A 14 T 11                                         210   E 46 A 18 D 13 S 9 Z 8 V 5                                                                       E 88 D 7                                               212   T 91              T 53 S 43                                              222   G 17 D 11 P 10 Y 9 V N 8                                                                         D 67 A 18                                              ______________________________________                                    

None of the entire combinations of surface residues in the human sequences are found in the murine sequences and vice versa (see lists at the end of this Example). However, the residues in individual positions appear to be conserved (see Table 1). There are few residues which differ significantly between the species; these are at positions 54 and 91 of the L chain and 168 and 216 of the H chain. Of these positions only position 216 is a non conservative (V to Y) mutation. Differences between human and murine antigenicities are therefore believed to arise from the combinations of residues in these positions.

In order to determine whether the mouse sequences are more distantly related to human F_(V) sequences than to other mouse F_(V) sequences, the homology was calculated using a Dayhoff mutation matrix (Dayhoff, M., Barker, W. and Hunt, L. (1983), Meth. Enz. 91, pp. 524-545). The homology was calculated between all the sequences in a pool of both human and mouse sequence patches made up of the surface accessible residues. The data was then represented as a density map (not shown) in which the sequences are plotted against each other. The density map can be used to discriminate "murine surfaces" from "human surfaces".

B) Reshaping of Antibody N901

In order to test the resurfacing approach suggested by the above analysis, three humanization experiments were set up. 1) Traditional loop grafting (Verhoeyen, M. E., Saunders, J. A., Broderick, E. L., Eida, S. J. and Badley, R. A. (1991), Disease markers 9, pp. 3-4) onto a human F_(V) framework of known structure (KOL). 2) Resurfacing approach using most similar chain. 3) Resurfacing approach using human sequences with most similar surface residues.

The antibody used was the murine anti-N901 antibody (Griffin et al. (1983), J. Imm. 130, pp. 2947-2951). The anti-N901 antibody (also referred to herein as the "N901 antibody") is available commercially from Coulter Corporation under the name NKH-1.

The alignment of the light chain sequences and heavy chain sequences in FIGS. 4A and 4B, respectively, show the original N901 antibody and the sequences used in each of the three approaches outlined here.

FIGS. 4A and 4B show alignments of sequences generated using the three methods of humanization. Sequences are: 1) Original rodent N901. 2+3) KOL (Marquart, M. Deisenhofer, J. and Huber, R. (1980), J. Mol. Biol. 141, pp. 369-391) and reshaped N901 using KOL surface. 4+5) Most homologous sequences, L(KV2F) (Klobeck, H., Meindl, A., Combriato, G., Solomon, A. and Zachau, H. (1985), Nucleic Acids Res., pp. 6499-6513) and H(G36005) (Schroeder, H. and Wang, J. (1990), Proc. Natl. Acad. Sci. U.S.A. 87) and reshaped N901 using these sequences. 6+7) Most homologous with respect to surface residues, L(KV4B) (Klobeck, H., Bronkamp, G., Combriato, G., Mocikat, R., Pohelnz, H. and Zachau, H. (1985), Nucleic Acids Res. 3, pp. 6515-6529) and H(PLO123) (Bird, J., Galili, N., Link, M., Sites, D. and Sklar, J. (1988), J. Exp. Med. 168, pp. 229-245), and reshaped N901 using these sequences. The numbering is the same as used in the antibody modelling program ABM (ABM is a trademark for commercial software, Oxford Molecular Ltd., Oxford, U.K.), which is based on structural conservation and not sequence homology as used by Padlan et al. (Kabat, E. A., Wu, T. T., Reid-Miller, M., Perry, H. M. and Gottesman, K. S. (1987), Sequences of Proteins of Immunological Interest. U.S. Department of Health and Human Services, Fourth Edition). The sequence changes which have to be introduced in order to reshape N901 with a given sequence are marked with bars, and back-mutations as determined from F_(V) models are marked with stars. The sequence homology of a given sequence to N901 is shown in brackets after each sequence.

(1) Classical Humanization

In classical humanization the rationale is to graft the rodent CDR's onto a framework of known structure, such that CDR-framework interactions can be accurately monitored by homology modelling. The model of the humanized antibody is compared to that of the original rodent antibody, and possible CDR interacting framework residues are back mutated (marked with `*` in alignment) in order to retain the three-dimensional shape of the CDR's. In this example the antibody KOL was used, giving a low homology score of only 77 and 46 in the heavy and light chains respectively.

(2) Most Similar Chain Resurfacing

A database of nonredundant human antibody sequences was compiled from available protein and nucleotide sequences. A total of 164 H and 129 L chains were sampled.

Each of the rodent chains, L and H, were then matched and the most similar human sequence found independently (G36005/KV2F) (Schroeder, H. and Wang, J. (1990), Proc. Natl. Acad. Sci. U.S.A. 87); Klobeck, H., Meindl, A., Combriato, G., Solomon, A. and Zachau, H. (1985), Nucleic Acids Res., pp. 6499-6513). Surface residues, as outlined in Table 1, were then changed in the rodent sequences to match those of the human sequences. Subsequently a model was built of the resurfaced antibody and compared to the model of the original rodent antibody and back mutation of any CDR interacting residues was performed.

(3) Most Similar Surface Replacement According to the Present Invention

This method is identical to the above method, except that the similarity is calculated only over the surface residues outlined in Table 1 above.

The same procedure of surface mutation and subsequent back mutation was performed as in the previous methods. In this case the chosen sequences were PLO123/KV4B (Bird, J., Galili, N., Link, M., Sites, D. and Sklar, J. (1988), J. Exp. Med. 168, pp. 229-245); Klobeck, H., Bronkamp, G., Combriato, G., Mocikat, R., Pohelnz, M. and Zachau, H. (1985), Nucleic Acids Res. 3, pp. 6515-6529).

The following lists show the surface residue patterns in mouse and human light and heavy chain antibody variable regions. The sequences are ordered on similarity to one another. There are no pattern matches between mouse and human sequences although there are matches within a species.

    __________________________________________________________________________     MOUSE LIGHT CHAIN SURFACE PATCHES                                              __________________________________________________________________________     1 KV5E$MOUSE                                                                              :KTSLRPGKGSSDYEKK*     (SEQ ID NO: 40)                              2 PL0101   :KTSLRPGKGSSEYEKK*     (SEQ ID NO: 41)                              3 N$1F19L  :QTSLRPDKGSSDHEKK*     (SEQ ID NO: 42)                              4 KV5U$MOUSE                                                                              :QTSLRPDKGSSDQEKK*     (SEQ ID NO: 43)                              5 MUSIGLDD :QSSLRPDKGSSDQEKK*     (SEQ ID NO: 44)                              6 PL0220   :QTSLRPDKGSSDPEKK*     (SEQ ID NO: 45)                              7 KV5J$MOUSE                                                                              :QTSLRPDKGSSDPZKK*     (SEQ ID NO: 46)                              8 MUSIGKABB                                                                               :QTSLRPDKGSSDPEKT*     (SEQ ID NO: 47)                              9 MUSIGKCLG                                                                               :QTSLRADKGSSDQEKK*     (SEQ ID NO: 48)                              10                                                                               MUSIGGVJ2                                                                               :QTSLRPDKGKSDSEKK*     (SEQ ID NO: 49)                              11                                                                               MUSIGKCRN                                                                               :QTSLRPARGSSDQEKK*     (SEQ ID NO: 50)                              12                                                                               MUSIGKCLF                                                                               :QTSLKPGRGSSDPEKK*     (SEQ ID NO: 51)                              13                                                                               MUSIGKACM                                                                               :QTSLRPGRGSSDTEKK*     (SEQ ID NO: 52)                              14                                                                               MUSIGKABE                                                                               :QISLRPGKGSSDSEKK*     (SEQ ID NO: 53)                              15                                                                               KV5P$MOUSE                                                                              :QTSLRPGKGDSDEDKK*     (SEQ ID NO: 54)                              16                                                                               MUSIGKCMK                                                                               :ETALRPGKGASDADKK*     (SEQ ID NO: 55)                              17                                                                               KV3D$MOUSE                                                                              :VTALRPGKGASDEDKK*     (SEQ ID NO: 56)                              18                                                                               MUSIGKAAW                                                                               :VTALRPGKGASDEEKK*     (SEQ ID NO: 57)                              19                                                                               KV3G$MOUSE                                                                              :VTALRPGKGASBABKK*     (SEQ ID NO: 58)                              20                                                                               KV3E$MOUSE                                                                              :VTALRPGKGASDEDDE*     (SEQ ID NO: 59)                              21                                                                               MUSIGKAAZ                                                                               :QTSLRPDKGSSDQETT*     (SEQ ID NO: 60)                              22                                                                               MUSIGKCNE                                                                               :QNSLTPGKGSSSPEKK*     (SEQ ID NO: 61)                              23                                                                               MUSIGKBA :VTKVRPGKGDSDSDKK*     (SEQ ID NO: 62)                              24                                                                               KV5A$MOUSE                                                                              :VTKVRPGKGDSDAEKK*     (SEQ ID NO: 63)                              25                                                                               MUSIGKV  :VTRVRPGKGDSDAEKK*     (SEQ ID NO: 64)                              26                                                                               MUSIGKCNM                                                                               :LTKVRPGKGDSDSEKK*     (SEQ ID NO: 65)                              27                                                                               MUSIGKCL1                                                                               :VTKVRPGKGDSDSEQK*     (SEQ ID NO: 66)                              28                                                                               KV5B$MOUSE                                                                              :VTKVRPEKGDSDAEKK*     (SEQ ID NO: 67)                              29                                                                               MUSIGKCSA                                                                               :VTKVRPEKGDSDSEKK*     (SEQ ID NO: 68)                              30                                                                               MUSIGKCSR                                                                               :VTKVSPGKGDSDAEKK*     (SEQ ID NO: 69)                              31                                                                               MUSIGKCST                                                                               :VTKVRSGKGESDAEKK*     (SEQ ID NO: 70)                              32                                                                               MUSIGKAB :VTSVKPGKGDSDAEKK*     (SEQ ID NO: 71)                              33                                                                               PL0014   :VSSVKPGKGDSDAEKK*     (SEQ ID NO: 72)                              34                                                                               MUSIGKACU                                                                               :VTSAKPGKGDSDAEKK*     (SEQ ID NO: 73)                              35                                                                               PS0023   :VSSAKPGKGDSDAEKK*     (SEQ ID NO: 74)                              36                                                                               N$2MCPL  :VTSARPGKGDSDAEKK*     (SEQ ID NO: 75)                              37                                                                               MUSIGKADV                                                                               :VSPAKPGKGDSDAEKK*     (SEQ ID NO: 76)                              38                                                                               MUSIGKCPF                                                                               :VTKARPGKGDSDVEKN*     (SEQ ID NO: 77)                              39                                                                               MUSIGLDE :VTLIPPGKGDSDAEKK*     (SEQ ID NO: 78)                              40                                                                               MUSIGKCNZ                                                                               :VTLLQPGKGDSDAEKK*     (SEQ ID NO: 79)                              41                                                                               B27887   :VTLLQPGKGDSDADKK*     (SEQ ID NO: 80)                              42                                                                               H28840   :VTLLQPGKGDSDAERK*     (SEQ ID NO: 81)                              43                                                                               KV2G$MOUSE                                                                              :VTLLQAGKGDSDAEKK*     (SEQ ID NO: 82)                              44                                                                               C27887   :VTLLQPGEGDSDAEKK*     (SEQ ID NO: 83)                              45                                                                               JL0029   :LTLLQPGNGDSDAEKK*     (SEQ ID NO: 84)                              46                                                                               MUSIGKAEH                                                                               :VTLLQPGKGDSDAEKI*     (SEQ ID NO: 85)                              47                                                                               PS0074   :VTLFQPGQGDSDPEKK*     (SEQ ID NO: 86)                              48                                                                               MUSIGKCNY                                                                               :VTLPQPGKGDSDAEKK*     (SEQ ID NO: 87)                              49                                                                               MUSIGKCNX                                                                               :VTLPQPGKGDWDAEKK*     (SEQ ID NO: 88)                              50                                                                               KV2D$MOUSE                                                                              :VTFLSPGQGDSDAEKK*     (SEQ ID NO: 89)                              51                                                                               MUSIGKADW                                                                               :ESSARPGKGDSDAEKK*     (SEQ ID NO: 90)                              52                                                                               KV2A$MOUSE                                                                              :VTLSSPGQGDSDAEKK*     (SEQ ID NO: 91)                              53                                                                               KV1A$MOUSE                                                                              :VTTAKPEKGDSDVEKK*     (SEQ ID NO: 92)                              54                                                                               F30534   :VTTPKPDKGDSDVEKK*     (SEQ ID NO: 93)                              55                                                                               MUSIGKCLO                                                                               :VTAPRPGKGASSAEKK*     (SEQ ID NO: 94)                              56                                                                               G27887   :VTAPKPGKGTSSAEKK*     (SEQ ID NO: 95)                              57                                                                               MUSIGVKV3                                                                               :VTTPKPGKGASSAEKK*     (SEQ ID NO: 96)                              58                                                                               MUSIGKCNA                                                                               :VSAPKPGKGASSAEKK*     (SEQ ID NO: 97)                              59                                                                               S03410   :VTAPRSGKGASSAEKK*     (SEQ ID NO: 98)                              60                                                                               B32456   :VTAPKSGKGASSAEKK*     (SEQ ID NO: 99)                              61                                                                               PL0013   :VTAPKPDKGVSSAEKK*     (SEQ ID NO: 100)                             62                                                                               MUSIGLAET                                                                               :VTAPKSEKGVSSAEKK*     (SEQ ID NO: 101)                             63                                                                               MUSIGVKV1                                                                               :FTAPKPGKGASSAEKK*     (SEQ ID NO: 102)                             64                                                                               KV6K$MOUSE                                                                              :LTAPKPGRGVSSAEKK*     (SEQ ID NO: 103)                             65                                                                               G30560   :VTAPKSGKGASSAEKR*     (SEQ ID NO: 104)                             66                                                                               MUSIGKBO :VSAPKPGKEGSSAEKK*     (SEQ ID NO: 105)                             67                                                                               MUSIGKCNB                                                                               :VTAPKPRKGASSAEKK*     (SEQ ID NO: 106)                             68                                                                               H33730   :VTFLSPGQGNSDAELP*     (SEQ ID NO: 107)                             69                                                                               MUSIGKCPC                                                                               :VTFLSPGQGNSDEDLP*     (SEQ ID NO: 108)                             70                                                                               KV2C$MOUSE                                                                              :VTLSSPQRGDSDAEKK*     (SEQ ID NO: 109)                             71                                                                               MUSIGLAV :VTAPKSSKGGSSAEKK*     (SEQ ID NO: 110)                             72                                                                               MUSIGKCNH                                                                               :QTSPTPGKGSSDPEKK*     (SEQ ID NO: 111)                             73                                                                               KV5R$MOUSE                                                                              :QISLIPGKGSYDDEKK*     (SEQ ID NO: 112)                             74                                                                               KV6E$MOUSE                                                                              :VTALKSGKGASSAEKK*     (SEQ ID NO: 113)                             75                                                                               MUSIGKCNI                                                                               :VTALKSDKGASSGEKK*     (SEQ ID NO: 114)                             76                                                                               MUSIGLDA :VTPPSPGQGDSAAEKK*     (SEQ ID NO: 115)                             77                                                                               C26317   :VTPPSPGQGDSAREKK*     (SEQ ID NO: 116)                             78                                                                               PS0073   :VTVRKPGKGDSSDEKK*     (SEQ ID NO: 117)                             79                                                                               A23986   :QTSVRLGQGSSDPEKK*     (SEQ ID NO: 118)                             80                                                                               MUSIGKABW                                                                               :KTSLRPWKGSSDSDKK*     (SEQ ID NO: 119)                             81                                                                               KV5D$MOUSE                                                                              :QTDVTQGQGSSQPEKK*     (SEQ ID NO: 120)                             82                                                                               MUSIGE6L :QTAVSQGQGSSQSEKK*     (SEQ ID NO: 121)                             83                                                                               MUSIGKCOE                                                                               :LTAPRTNRGSSDSEKK*     (SEQ ID NO: 122)                             84                                                                               MUSIGKCKN                                                                               :VTAPSSHRGSSDTEKK*     (SEQ ID NO: 123)                             85                                                                               MUSIGLVD :LLSLSPLKGDSDPEKV*     (SEQ ID NO: 124)                             86                                                                               S06822   :VTAPTPDTGAIKTEKL*     (SEQ ID NO: 125)                             87                                                                               S06821   :VTIPTPDTGAIKTEKL*     (SEQ ID NO: 126)                             88                                                                               MUSIGLAS :AVSPTPDTGAIKTEKL*     (SEQ ID NO: 127)                             89                                                                               MUSIGLAR :AVSPTPDTGAIKTEKL*     (SEQ ID NO: 128)                             90                                                                               LV2B$MOUSE                                                                              :AVSPTPDTGVIKTEKL*     (SEQ ID NO: 129)                             91                                                                               MUSIGLAN :AVSPTPDTGAIKTEPS*     (SEQ ID NO: 130)                             __________________________________________________________________________     HUMAN LIGHT CHAIN SURFACE PATCHES                                              __________________________________________________________________________     1 LV4A$HUMAN                                                                              :YLPPTPGVIRSTAMKL*     (SEQ ID NO: 131)                             2 LV4B$HUMAN                                                                              :YLPPTPGVIRSTAMRL*     (SEQ ID NO: 132)                             3 LV4E$HUMAN                                                                              :YLPPTPGLIRSTSMKL*     (SEQ ID NO: 133)                             4 LV4D$HUMAN                                                                              :YLPPTPGLIRSTSVKL*     (SEQ ID NO: 134)                             5 LV4C$HUMAN                                                                              :YLPPTPGVIRSTAEKL*     (SEQ ID NO: 135)                             6 LV5A$HUMAN                                                                              :YLPPTPGVIRSTAGKL*     (SEQ ID NO: 136)                             7 LV7A$HUMAN                                                                              :YLPATPGVVRSSAGML*     (SEQ ID NO: 137)                             8 LV2G$HUMAN                                                                              :SLPPSPGKVRSTAEKL*     (SEQ ID NO: 138)                             9 LV2I$HUMAN                                                                              :SLPPSPGKVRSTANKL*     (SEQ ID NO: 139)                             10                                                                               N$2RHE   :SLPPRPGKVRSSSEKL*     (SEQ ID NO: 140)                             11                                                                               HUMIGLAN :SLPPRPGKVRSSSDKL*     (SEQ ID NO: 141)                             12                                                                               LV1A$HUMAN                                                                              :SLPPRPGRVRSSSEKL*     (SEQ ID NO: 142)                             13                                                                               LV1B$HUMAN                                                                              :SLPPRPGKVRSSSEQL*     (SEQ ID NO: 143)                             14                                                                               LV1F$HUMAN                                                                              :SLPPRPGKVRSSSETL*     (SEQ ID NO: 144)                             15                                                                               LV1C$HUMAN                                                                              :SLPPKPGKIRSSTGKL*     (SEQ ID NO: 145)                             16                                                                               A29700   :SLPPKPGRIRSSTGKL*     (SEQ ID NO: 146)                             17                                                                               HUMIGLAM4                                                                               :SLPPKPGKIRSSTGQL*     (SEQ ID NO: 147)                             18                                                                               LV1D$HUMAN                                                                              :SLPPEPGKIRSSTGRL*     (SEQ ID NO: 148)                             19                                                                               LV2K$HUMAN                                                                              :SLAPSPGKIRSTAEKL*     (SEQ ID NO: 149)                             20                                                                               LV1I$HUMAN                                                                              :SLPPRPGKIRSSTGNV*     (SEQ ID NO: 150)                             21                                                                               LV2E$HUMAN                                                                              :SLRPSPGKVRSTAEKL*     (SEQ ID NO: 151)                             22                                                                               LV2D$HUMAN                                                                              :SLRPSPGKVRSTADKL*     (SEQ ID NO: 152)                             23                                                                               LV2C$HUMAN                                                                              :SLRPSPGKVRSTAENL*     (SEQ ID NO: 153)                             24                                                                               LV2J$HUMAN                                                                              :SLRPSPGKVRSAVEKL*     (SEQ ID NO: 154)                             25                                                                               LV1E$HUMAN                                                                              :SLPPRPGK-RSSAEKL*     (SEQ ID NO: 155)                             26                                                                               LV2B$HUMAN                                                                              :SLAPSPGKVRSTVERL*     (SEQ ID NO: 156)                             27                                                                               N$1MCWW  :SLAPSPDKIRSTPDKL*     (SEQ ID NO: 157)                             28                                                                               LV2H$HUMAN                                                                              :SLALSPGKVRSTAEKL*     (SEQ ID NO: 158)                             29                                                                               N$3MCG2  :SLPLSAGKVRSTAEKL*     (SEQ ID NO: 159)                             30                                                                               LV2A$HUMAN                                                                              :SLAPSPGKVRSTAEYL*     (SEQ ID NO: 160)                             31                                                                               S02083   :SLPLTPGLIRSTAEKL*     (SEQ ID NO: 161)                             32                                                                               HUMIGLAM2                                                                               :SLPLTPRVIRSTAEKL*     (SEQ ID NO: 162)                             33                                                                               LV6C$HUMAN                                                                              :FLHPTPGTDSSSTEKL*     (SEQ ID NO: 163)                             34                                                                               LV6D$HUMAN                                                                              :FLLPTPGTDSSSTERL*     (SEQ ID NO: 164)                             35                                                                               LV6E$HUMAN                                                                              :FLHPTRVTDSSSTEKL*     (SEQ ID NO: 165)                             36                                                                               LV6B$HUMAN                                                                              :LLPPTPGTNSSSNDKL*     (SEQ ID NO: 166)                             37                                                                               HUMIGLK5G                                                                               :VLPLSPHRIRSESENL*     (SEQ ID NO: 167)                             38                                                                               HUMIGLVC :SLAPSPAKFRSTAERD*     (SEQ ID NO: 168)                             39                                                                               HUMIGVLLS                                                                               :VTAPRPGRIRSDPEKK*     (SEQ ID NO: 169)                             40                                                                               HUMIGKAX :VTAPRPGRVRSDPEKK*     (SEQ ID NO: 170)                             41                                                                               E30609   :VTGPRPGRIRSDPEKK*     (SEQ ID NO: 171)                             42                                                                               KV3B$HUMAN                                                                              :VTGPRPGRIRSDPDKK*     (SEQ ID NO: 172)                             43                                                                               G30607   :VTGPRPGRVRSDPEKK*     (SEQ ID NO: 173)                             44                                                                               KV3M$HUMAN                                                                              :VTGPRPGRIRSDPXKK*     (SEQ ID NO: 174)                             45                                                                               KV3H$HUMAN                                                                              :VTAPRPGRIRSESERK*     (SEQ ID NO: 175)                             46                                                                               KV3K$HUMAN                                                                              :VTGPSRGRIRSDPEKK*     (SEQ ID NO: 176)                             47                                                                               KV3F$HUMAN                                                                              :VTVPRPSRIRSESERK*     (SEQ ID NO: 177)                             48                                                                               B26555   :VTAPGPGRIRSESERK*     (SEQ ID NO: 178)                             49                                                                               KV1Q$HUMAN                                                                              :QTSVRPGRVRSDPERK*     (SEQ ID NO: 179)                             50                                                                               KV1W$HUMAN                                                                              :QTSVRPGKVRSDPERK*     (SEQ ID NO: 180)                             51                                                                               KV1M$HUMAN                                                                              :QTSVRPGKVRSDPEKK*     (SEQ ID NO: 181)                             52                                                                               KV1R$HUMAN                                                                              :QTSVRPGKVRSEPEKK*     (SEQ ID NO: 182)                             53                                                                               KV1F$HUMAN                                                                              :QTSVRPGKVRSEPDKK*     (SEQ ID NO: 183)                             54                                                                               KV1G$HUMAN                                                                              :QTSVRPGKVRAEPEKK*     (SEQ ID NO: 184)                             55                                                                               KV1K$HUMAN                                                                              :QTSVRPGKVRSBPZKK*     (SEQ ID NO: 185)                             56                                                                               KV1D$HUMAN                                                                              :QTSVRPGKVRSDPBKK*     (SEQ ID NO: 186)                             57                                                                               KV1H$HUMAN                                                                              :QTSVRPGQVRSDPERK*     (SEQ ID NO: 187)                             58                                                                               KV1B$HUMAN                                                                              :QTSVRPGKVRSHPEKK*     (SEQ ID NO: 188)                             59                                                                               B27585   :QTSVRPGNVRSDPDKK*     (SEQ ID NO: 189)                             60                                                                               N$1REIA  :QTSVRPGKVRSDPEKT*     (SEQ ID NO: 190)                             61                                                                               KV1X$HUMAN                                                                              :QTSVRPGTVRSEPEKK*     (SEQ ID NO: 191)                             62                                                                               KV1L$HUMAN                                                                              :QTSVRPEKVRSEPDKK*     (SEQ ID NO: 192)                             63                                                                               IMGL38   :QTSVRPGKVRSESDKK*     (SEQ ID NO: 193)                             64                                                                               A27585   :QTSVRPGEVRSEPDKK*     (SEQ ID NO: 194)                             65                                                                               KV1N$HUMAN                                                                              :QTSVRPGBVRSBPZRK*     (SEQ ID NO: 195)                             66                                                                               KV1C$HUMAN                                                                              :QTSVSPGKVRSDPEKK*     (SEQ ID NO: 196)                             67                                                                               KV1V$HUMAN                                                                              :QTSVRPGKVNSDPEKK*     (SEQ ID NO: 197)                             68                                                                               KV1T$HUMAN                                                                              :QTSVRPGKVRSDPDTK*     (SEQ ID NO: 198)                             69                                                                               KV1U$HUMAN                                                                              :QTSVRPKKVRSDPZKK*     (SEQ ID NO: 199)                             70                                                                               KV1A$HUMAN                                                                              :QTSVRPKKVRFDPEKK*     (SEQ ID NO: 200)                             71                                                                               KV1S$HUMAN                                                                              :QTSVRSGKVRSEPETK*     (SEQ ID NO: 201)                             72                                                                               KV4A$HUMAN                                                                              :VTNLRPGKVRSDAEKK*     (SEQ ID NO: 202)                             73                                                                               KV4C$HUMAN                                                                              :VTDLRPGKVRSDAEKK*     (SEQ ID NO: 203)                             74                                                                               HUMIGK2A1                                                                               :QTSVSPGNIRSESDKK*     (SEQ ID NO: 204)                             75                                                                               HUMIGKBA :KTSVTPGKFRSEPEKK*     (SEQ ID NO: 205)                             76                                                                               HUMIGKBC :VTLLPPGRVRSDAEKK*     (SEQ ID NO: 206)                             77                                                                               KV2B$HUMAN                                                                              :VTLLPPGEVRSDAEKK*     (SEQ ID NO: 207)                             78                                                                               KV2D$HUMAN                                                                              :VTLPPPGZVRSDAERK*     (SEQ ID NO: 208)                             79                                                                               KV2C$HUMAN                                                                              :VTLPPPGZVRSBAZNK*     (SEQ ID NO: 209)                             80                                                                               KV2E$HUMAN                                                                              :VTLPPPQQVRSDAEKK*     (SEQ ID NO: 210)                             81                                                                               S03876   :VTLPPPGQVTSDAEKK*     (SEQ ID NO: 211)                             82                                                                               KV2A$HUMAN                                                                              :VTLPPAGQVRSDAEKR*     (SEQ ID NO: 212)                             83                                                                               HUMIGLAM5                                                                               :ALSPSSGQSSSASERL*     (SEQ ID NO: 213)                             __________________________________________________________________________     MOUSE HEAVY CHAIN SURFACE PATCHES                                              __________________________________________________________________________     1 MUSIGHIT :EKVGGLQPGRGTPGKASRGDSQRPES*                                                                          (SEQ ID NO: 214)                             2 MUSIGHIU :EKVGGLQPGRGTPGKVSRGDSQRPES*                                                                          (SEQ ID NO: 215)                             3 MUSIGHIV :EKVGGLQPGTGAPGKASRGDSQRPES*                                                                          (SEQ ID NO: 216)                             4 MUSIGHYM :EKVGGLQPGRGTPGKASKGNSQRAES*                                                                          (SEQ ID NO: 217)                             5 PU0003   :EKMGGLQPGRGTPGKASKGNSQRAES*                                                                          (SEQ ID NO: 218)                             6 MUSIGHF0 :EKVGGLQPGRGTPGKASKGTSQRAES*                                                                          (SEQ ID NO: 219)                             7 A30515   :EKVGGLQPGRGTPGKASKGTSQRAET*                                                                          (SEQ ID NO: 220)                             8 PL0018   :EKVGGLKPGRGTPGKASKGTSQRAET*                                                                          (SEQ ID NO: 221)                             9 MUSIGHFK :ENVGGLQPGRGTPGKASKGTSQRAET*                                                                          (SEQ ID NO: 222)                             10                                                                               MUSIGHFQ :EKVGGLQSGRGTPGKASKGTSQRAET*                                                                          (SEQ ID NO: 223)                             11                                                                               PU0001   :EKVGGLQSGRGTPGKASKGTSQRAES*                                                                          (SEQ ID NO: 224)                             12                                                                               E30540   :EKVGGLQPGRGTPGKASKGISQRAER*                                                                          (SEQ ID NO: 225)                             13                                                                               HV17$MOUSE                                                                              :EKVGGLQPGRGTPGKSAKGBSZRAQS*                                                                          (SEQ ID NO: 226)                             14                                                                               MUSIGHLN :EKVGGLQPGSGTPGKASKGNSQRAES*                                                                          (SEQ ID NO: 227)                             15                                                                               MUSIGHKG :EKVGGLQPGSGTPGKASKGSSQRAES*                                                                          (SEQ ID NO: 228)                             16                                                                               PU0004   :EKVGGLQPGRGTPRKASKGNSQRAES*                                                                          (SEQ ID NO: 229)                             17                                                                               MUSIGHKJ :EKMGNLQPGSGTPGKASKGNSQRPDS*                                                                          (SEQ ID NO: 230)                             18                                                                               HV56$MOUSE                                                                              :EKVGGLKPGKGTPEKDSKGNARRSET*                                                                          (SEQ ID NO: 231)                             19                                                                               C27888   :EKVGGLKPGKGAPEKDSKGNARRSET*                                                                          (SEQ ID NO: 232)                             20                                                                               MUSIGHAAF                                                                               :EKVGGLKPGKGTPERDSKGNARRSET*                                                                          (SEQ ID NO: 233)                             21                                                                               PH0097   :DKVGGLKPGKGTPEKDSKGNAKRSET*                                                                          (SEQ ID NO: 234)                             22                                                                               E27888   :DKVGGLKPGKGTPEKDSKGNAKKSET*                                                                          (SEQ ID NO: 235)                             23                                                                               MUSIGHJB :DKVGGLKPGKGTPDKDNKGNAKKSET*                                                                          (SEQ ID NO: 236)                             24                                                                               MUSIGHADL                                                                               :EKVGGLTPGKGTPEKDSKGNGRRSET*                                                                          (SEQ ID NO: 237)                             25                                                                               A27888   :EMVGGLKPGKGTPEKDSKGNDRRSET*                                                                          (SEQ ID NO: 238)                             26                                                                               H27887   :EMVGGLKPGKGTPEKDSKGNDKRSET*                                                                          (SEQ ID NO: 239)                             27                                                                               B27888   :EMVGGLKPGKGTPEKDSKGNAKRSET*                                                                          (SEQ ID NO: 240)                             28                                                                               B27889   :EQVGGLKPGKGTPEKDSKGNAKKSET*                                                                          (SEQ ID NO: 241)                             29                                                                               D27889   :EQVGGLKPGKGTPEKDTKGNAKKSET*                                                                          (SEQ ID NO: 242)                             30                                                                               HV55$MOUSE                                                                              :EQVGGLKPGKGAPEKDTKGNAKKSET*                                                                          (SEQ ID NO: 243)                             31                                                                               MUSIGHAGT                                                                               :EKVGGLQPGKGTPEKDSKGNAKKSET*                                                                          (SEQ ID NO: 244)                             32                                                                               MUSIGVH50                                                                               :EKVGGLQPGKGTPEKDTKGNAKKSET*                                                                          (SEQ ID NO: 245)                             33                                                                               MUSIGHIW :EKVGGLQPGRGTPEKDTKGNAKKSET*                                                                          (SEQ ID NO: 246)                             34                                                                               MUSIGHAGZ                                                                               :EKVGGLQPGKGSPEKDSKGNAKKSET*                                                                          (SEQ ID NO: 247)                             35                                                                               PH0098   :DKMGGLKPGKGTPEKDSKGNAKQSET*                                                                          (SEQ ID NO: 248)                             36                                                                               MUSIGHIN :EQVGGLQPGKGTPDKDSKGNAKKSET*                                                                          (SEQ ID NO: 249)                             37                                                                               MUSIGHAGY                                                                               :EKVGGLQPGKGTPEKDSKGNAEKSET*                                                                          (SEQ ID NO: 250)                             38                                                                               MUSIGHMP :EQVGDLKPGKGTPEKDTKGNARRSET*                                                                          (SEQ ID NO: 251)                             39                                                                               D27888   :ENVGDLKPGKGAPEKDSKGNARRSET*                                                                          (SEQ ID NO: 252)                             40                                                                               MUSIGHIP :EQVGGLQPGKGTSDKDSKGNAKKSET*                                                                          (SEQ ID NO: 253)                             41                                                                               MUSIGHAGS                                                                               :EQVGGLQPGKGTPEKDSKGNAKKSGT*                                                                          (SEQ ID NO: 254)                             42                                                                               HV16$MOUSE                                                                              :DQVGGLQPGKGTPEKDTKGNPKRSET*                                                                          (SEQ ID NO: 255)                             43                                                                               B34871   :DQVGGLQPGQGTPEKNTKGNPKRSDT*                                                                          (SEQ ID NO: 256)                             44                                                                               PH0094   :EKVGGLQPGKGTSEKDIKGNAKKSET*                                                                          (SEQ ID NO: 257)                             45                                                                               PH0096   :DKVGGLKPGKRTPEKDNKGNAKKSET*                                                                          (SEQ ID NO: 258)                             46                                                                               MUSIGVH62                                                                               :DKVGGLKLGKGTPEKDTKGNAKKSET*                                                                          (SEQ ID NO: 259)                             47                                                                               MUSIGHAGR                                                                               :EKVGGLQPGKGTPEKDSKGNANTSET*                                                                          (SEQ ID NO: 260)                             48                                                                               HV58$MOUSE                                                                              :EHVGGLKPGKGTPEKDSKGNAGRSET*                                                                          (SEQ ID NO: 261)                             49                                                                               H27888   :EQVGGLQPGNGTPEKDTTGNAKRSET*                                                                          (SEQ ID NO: 262)                             50                                                                               HV34$MOUSE                                                                              :EKEGGLQPGKGTPEKESKGDSKRAET*                                                                          (SEQ ID NO: 263)                             51                                                                               HV33$MOUSE                                                                              :EKEGGLQPGKGTPEKESKGDSKRPET*                                                                          (SEQ ID NO: 264)                             52                                                                               MUSIGHZAB                                                                               :EKEGGLQPGKGSPEKESKGDSKRAET*                                                                          (SEQ ID NO: 265)                             53                                                                               N$4FABH  :EKDGGLQPGKGTPEKDSKGDSKRVEM*                                                                          (SEQ ID NO: 266)                             54                                                                               I27888   :EQVGGLKPGRGTPEKDTTGDAQRSET*                                                                          (SEQ ID NO: 267)                             55                                                                               G27888   :EQVGGLKPGRGTPEKDTTGNAKGSET*                                                                          (SEQ ID NO: 268)                             56                                                                               HV59$MOUSE                                                                              :EKVGGSKPGKGTPEKDSKGNAKTSET*                                                                          (SEQ ID NO: 269)                             57                                                                               MUSIGHOE :SDQGGLKPGKGTPEKDTKGNARRSES*                                                                          (SEQ ID NO: 270)                             58                                                                               N$2FVWH  :EKIGGLQPGKGDPGKPSKDNAKRSET*                                                                          (SEQ ID NO: 271)                             59                                                                               MUSIGHJT :EKLGGLQPGKGDPGKPSKDNAKRSET*                                                                          (SEQ ID NO: 272)                             60                                                                               MUSIGHLY :EKLGGLQPGKGDPGKPFKDNAKRSET*                                                                          (SEQ ID NO: 273)                             61                                                                               S06816   :EKLGGLQPGKGDPGKLMKENAKRSET*                                                                          (SEQ ID NO: 274)                             62                                                                               S06817   :ENLGGLQPGKGDPGKLKXENAKRPET*                                                                          (SEQ ID NO: 275)                             63                                                                               MUSIGHAAI                                                                               :EKLGGLQPGNGDLGKPSKDNAKRSET*                                                                          (SEQ ID NO: 276)                             64                                                                               HV42$MOUSE                                                                              :EKLGPLQLGKGDPGKPSKDDAKRSET*                                                                          (SEQ ID NO: 277)                             65                                                                               MUSIGHAAL                                                                               :EQLGGLQPGGGTPGKPSKDNDKRSET*                                                                          (SEQ ID NO: 278)                             66                                                                               MUSIGHABO                                                                               :EQLGGLQPGGGTPGKASKDNDKRSET*                                                                          (SEQ ID NO: 279)                             67                                                                               MUSIGHEG :EQVGGLKARKGTPEKDTTGNAKRSET*                                                                          (SEQ ID NO: 280)                             68                                                                               MUSIGHWN :EMVGVLEPGKGTPEKRQEGNAKRSET*                                                                          (SEQ ID NO: 281)                             69                                                                               MUSIGKCLT                                                                               :EQVGGLQPKKGSPGKDSKDDSQKTET*                                                                          (SEQ ID NO: 282)                             70                                                                               MUSIGHZAE                                                                               :EQVGGLQPKKGSPGKDSKDDSQKTER*                                                                          (SEQ ID NO: 283)                             71                                                                               MUSIGHAAD                                                                               :QQVPELKPGRGTPGKEDKGTSARNDT*                                                                          (SEQ ID NO: 284)                             72                                                                               MUSIGHAAW                                                                               :QQVPELKPGKGTPGKDDKGTSAKNET*                                                                          (SEQ ID NO: 285)                             73                                                                               MUSIGHAMA                                                                               :QQVPELKPGKGTPGKDDKGTSAKNEM*                                                                          (SEQ ID NO: 286)                             74                                                                               MUSIGHXZ :QQKPELKPGKGSPGQEKKSTSSTSET*                                                                          (SEQ ID NO: 287)                             75                                                                               A30502   :EQQPELKPGKGTPGQEKKGKSSTSES*                                                                          (SEQ ID NO: 288)                             76                                                                               MUSIGHAAG                                                                               :EQQPELRPGKGTPGQEKKGKSSTSES*                                                                          (SEQ ID NO: 289)                             77                                                                               B30502   :EQQPELKPGKGTPGQEKKGKSSASES*                                                                          (SEQ ID NO: 290)                             78                                                                               MUSIGHADG                                                                               :EQQPELKPGKGTPGKQKKGKSSTSES*                                                                          (SEQ ID NO: 291)                             79                                                                               MUSIGHFV :EQQPELKPGKGTHGKQKKGKSSTSES*                                                                          (SEQ ID NO: 292)                             80                                                                               MUSIGHAANA                                                                              :EQQPELKPGKGSHGKQKKGKSSTSES*                                                                          (SEQ ID NO: 293)                             81                                                                               MUSIGHZR :EQQPELKPGKGSHGKQKKGKSSASES*                                                                          (SEQ ID NO: 294)                             82                                                                               MUSIGHAI :EQQPELKPGKGTHGKQKKGKSSTFES*                                                                          (SEQ ID NO: 295)                             83                                                                               MUSIGHALA                                                                               :EQQPELKPGKGTHGKQKQGKSSTFES*                                                                          (SEQ ID NO: 296)                             84                                                                               PL0011   :EQQPELKPGKGTHGKEKKDKSSTSES*                                                                          (SEQ ID NO: 297)                             85                                                                               MUSIGKCLS                                                                               :EQQAELKPGKGSHGKQKKGKSSTSES*                                                                          (SEQ ID NO: 298)                             86                                                                               MUSIGHADY                                                                               :EQQPELKPGKGTHGKQKKSNSSTSES*                                                                          (SEQ ID NO: 299)                             87                                                                               MUSIGHWVX                                                                               :QQQAELRPGKGAPGQEKKGKSSTSES*                                                                          (SEQ ID NO: 300)                             88                                                                               MUSIGHADO                                                                               :QQQAELRPGKGAPGQEKKGKSSTSDS*                                                                          (SEQ ID NO: 301)                             89                                                                               MUSIGHVEM                                                                               :QQQAELRPGKGVPGQEKKGKSSTSDS*                                                                          (SEQ ID NO: 302)                             90                                                                               A24672   :QQQPELKPGKGAPGKGKKGKSSTSES*                                                                          (SEQ ID NO: 303)                             91                                                                               MUSIGHJG :QQQPELKPGKGAPGKGKKDKSSTSES*                                                                          (SEQ ID NO: 304)                             92                                                                               JL0044   :EQQPEAKPGKGTHGKQKKGKSSTSDS*                                                                          (SEQ ID NO: 305)                             93                                                                               MUSIGHBA :QQQAELKPGKGTHGKEKKDKSSTSDS*                                                                          (SEQ ID NO: 306)                             94                                                                               MUSIGHAGP                                                                               :QQQAELRPGKGAPGQGKKGKSSTSES*                                                                          (SEQ ID NO: 307)                             95                                                                               MUSIGHVBK                                                                               :QQQAELKPGRGTPGQEKKGKSSTSES*                                                                          (SEQ ID NO: 308)                             96                                                                               A36194   :EQQAELRAGKGTPGQEKKGKSSTSES*                                                                          (SEQ ID NO: 309)                             97                                                                               MUSIGHVBJ                                                                               :EQQAELRPGKGTPGQEKKGTSSTSES*                                                                          (SEQ ID NO: 310)                             98                                                                               MUSIGHADV                                                                               :QQQAELRPGKGTPGHEKKGTSSTSES*                                                                          (SEQ ID NO: 311)                             99                                                                               MUSIGHAAT                                                                               :QQQAELKPGKGTPGHEKKGTSSTSES*                                                                          (SEQ ID NO: 312)                             100                                                                              MUSIGHJL :QQQAELRPGKGTPGHENKGTSSTSES*                                                                          (SEQ ID NO: 313)                             101                                                                              MUSIGHABM                                                                               :QQQAEVRPGKGTPGHEKKGTSSTSES*                                                                          (SEQ ID NO: 314)                             102                                                                              MUSIGHFU :QQQAELKPGKGTPGHENKGTSSTSES*                                                                          (SEQ ID NO: 315)                             103                                                                              MUSIGHZZB                                                                               :QQQAELRPGKGTPGQQKKGKSSASES*                                                                          (SEQ ID NO: 316)                             104                                                                              HV06$MOUSE                                                                              :HQQAELKPGKGTPGQQKKGKSSTSES*                                                                          (SEQ ID NO: 317)                             105                                                                              MUSIGHRD :EQQVELRAGKGTPGQEKKGKSSTSES*                                                                          (SEQ ID NO: 318)                             106                                                                              MUSIGHVBH                                                                               :EQQAELRPGKGTPGQEKQGTSSTSES*                                                                          (SEQ ID NO: 319)                             107                                                                              HV01$MOUSE                                                                              :EQQAELRPGKGTPGHDNKGTSSTSES*                                                                          (SEQ ID NO: 320)                             108                                                                              MUSIGHADN                                                                               :QQQAEVRPGKGTPGHEKKGRSSTSES*                                                                          (SEQ ID NO: 321)                             109                                                                              HV05$MOUSE                                                                              :QQQAELRPGKGTPGQQKKDKSSTSES*                                                                          (SEQ ID NO: 322)                             110                                                                              MUSIGHAEF                                                                               :QQQAELKPGKGTPGQQKKDKSSTSES*                                                                          (SEQ ID NO: 323)                             111                                                                              MUSIGHAAN                                                                               :QQQAELKPGKGTPGQQKKDKSSTSDS*                                                                          (SEQ ID NO: 324)                             112                                                                              MUSIGHAAB                                                                               :QQQAELKPGKGSPGQQKKDKSSTSES*                                                                          (SEQ ID NO: 325)                             113                                                                              C30560   :QHQAELKPGKGTPGQQKKNKSSTSES*                                                                          (SEQ ID NO: 326)                             114                                                                              PS0024   :QQQAELKPGKGTPGQQNKDKSSTSES*                                                                          (SEQ ID NO: 327)                             115                                                                              MUSIGHRG :EQQAELRAGKGIPGQEKKGKSSTSES*                                                                          (SEQ ID NO: 328)                             116                                                                              MUSIGHAAE                                                                               :QQQAELKPGKGTPGQEKKSKSSTSES*                                                                          (SEQ ID NO: 329)                             117                                                                              MUSIGHLX :QQQSELKPGKGTPGQEKKSKSSTSES*                                                                          (SEQ ID NO: 330)                             118                                                                              HV04$MOUSE                                                                              :QQQTELKPGKGTPGQEKKSKSSTSES*                                                                          (SEQ ID NO: 331)                             119                                                                              MUSIGHVBG                                                                               :EQQAELRTGKGTPGQERKGKSSTSES*                                                                          (SEQ ID NO: 332)                             120                                                                              MUSIGHMX :QQQAELKPGKGTPGQQKKDKSSTFES*                                                                          (SEQ ID NO: 333)                             121                                                                              MUSIGHAAR                                                                               :EQQAELRPGTGAPGQEKKGKSSTSES*                                                                          (SEQ ID NO: 334)                             122                                                                              HV15$MOUSE                                                                              :QQQPEVRPGKGTHAKQKKGKSSTSES*                                                                          (SEQ ID NO: 335)                             123                                                                              MUSIGHAAU                                                                               :QQQPEVRPGKDTHAKQKKGKSSTSES*                                                                          (SEQ ID NO: 336)                             124                                                                              MUSIGHVBO                                                                               :QQQAELKPGKGTPEQEKKGKSSTSES*                                                                          (SEQ ID NO: 337)                             125                                                                              A26405   :EQQTELRAGKGTPGQEKKGRSSTSEA*                                                                          (SEQ ID NO: 338)                             126                                                                              HV10$MOUSE                                                                              :QQQAELKPGKGTPGREKKSKPSTSES*                                                                          (SEQ ID NO: 339)                             127                                                                              MUSIG3B44                                                                               :QQQSELKPGKGTPGREKKSKPSTSES*                                                                          (SEQ ID NO: 340)                             128                                                                              MUSIG3B62                                                                               :QQRAELKPGKDTPGREKKNKPSTSES*                                                                          (SEQ ID NO: 341)                             129                                                                              HV09$MOUSE                                                                              :QQQAELKPGKGTPGREKKSTSSTSES*                                                                          (SEQ ID NO: 342)                             130                                                                              MUSIGKCLP                                                                               :QQQAELKPGKGTPGQEKKSTSSTSDS*                                                                          (SEQ ID NO: 343)                             131                                                                              MUSIGBH  :QQQAELRPGKGTPIQQKKDKSSTSES*                                                                          (SEQ ID NO: 344)                             132                                                                              HV11$MOUSE                                                                              :QQQAEFKPGKGTPGREHRSKPSTSES*                                                                          (SEQ ID NO: 345)                             133                                                                              MUSIGHMC :QQQAELRPGKGALGQEKKGKSSTSDS*                                                                          (SEQ ID NO: 346)                             134                                                                              MUSIGHAGW                                                                               :QQQPEVKPGKGAPKGKNTDKSSTSES*                                                                          (SEQ ID NO: 347)                             135                                                                              MUSIGHRF :EQQAEVRAGKGSPGQEKKGKSSTSES*                                                                          (SEQ ID NO: 348)                             136                                                                              MUSIGHVAD                                                                               :QQLAELKPGKGTPGHEKKGISSTSES*                                                                          (SEQ ID NO: 349)                             137                                                                              MUSIGHVAF                                                                               :QQQAELKPGKGKPEQEKKGTSSTSES*                                                                          (SEQ ID NO: 350)                             138                                                                              PL0012   :QQQPELKPGKGRNGKENKGKSSTSES*                                                                          (SEQ ID NO: 351)                             139                                                                              MUSIGGVD2                                                                               :QQQTELRPGRGTTGQERKGKSSTSES*                                                                          (SEQ ID NO: 352)                             140                                                                              S06824   :QHQAELKPGKGTPGHENKVTSSTSES*                                                                          (SEQ ID NO: 353)                             141                                                                              MUSIGHRD :EQQAELRAGKGTPGQEQKAKSSTSES*                                                                          (SEQ ID NO: 354)                             142                                                                              MUSIGHAAE                                                                               :QQQAELKPGKGTPGQQKTGTSSTTES*                                                                          (SEQ ID NO: 355)                             143                                                                              MUSIGHHS :QQQAELKPGKGNPGQEKKSTSSASES*                                                                          (SEQ ID NO: 356)                             144                                                                              MUSIGHAXA                                                                               :EQQTVLRPGKGTPGQQKKGTSATNES*                                                                          (SEQ ID NO: 357)                             145                                                                              HV50$MOUSE                                                                              :QQLTELKPGNGTPGQEKKSKSSTSES*                                                                          (SEQ ID NO: 358)                             146                                                                              MUSIGHVBP                                                                               :QQQSVLRPGKGTPGQEKKGTSSTSKS*                                                                          (SEQ ID NO: 359)                             147                                                                              PH0100   :LQQPVLKPGKGSHGKQKKDKSSTSES*                                                                          (SEQ ID NO: 360)                             148                                                                              MUSIGHAYA                                                                               :EQQPETKPGKGTLGKQKKSKSSTSES*                                                                          (SEQ ID NO: 361)                             149                                                                              MUSIGHCP2                                                                               :QQQAELKPGQGTPGQEKKNKSSTPEF*                                                                          (SEQ ID NO: 362)                             150                                                                              MUSIGHDZ :EQQAELRPGKGNPEQPKQGTSSTSET*                                                                          (SEQ ID NO: 363)                             151                                                                              MUSIGHNPI                                                                               :EQQAELRPGKGNPEQPKQGTSTTSET*                                                                          (SEQ ID NO: 364)                             152                                                                              S06823   :EQQAELKPGKGNPEQPKQGTSSTSET*                                                                          (SEQ ID NO: 365)                             153                                                                              MUSIGHASA                                                                               :EQQAELKPGKGNPEQPKQDTSSTSET*                                                                          (SEQ ID NO: 366)                             154                                                                              S03484   :EQQAELKPGKGNPEQPKQGTSSTSGT*                                                                          (SEQ ID NO: 367)                             155                                                                              MUSIGHVAA                                                                               :EQQAEVKPGKGNPEQPKQGTSSTSET*                                                                          (SEQ ID NO: 368)                             156                                                                              MUSIGHNPD                                                                               :EQQAELRPGKGNPEQPKQVTSSTSET*                                                                          (SEQ ID NO: 369)                             157                                                                              MUSIGHNPB                                                                               :EQQAELRPGKGNPEQPKQITSSTSET*                                                                          (SEQ ID NO: 370)                             158                                                                              MUSIGHEC :EQQAELRPGRGNPEQPKQVTSSTSET*                                                                          (SEQ ID NO: 371)                             159                                                                              MUSIGHNPC                                                                               :EQQAELRPGRGNPEQPKHVTSSTSET*                                                                          (SEQ ID NO: 372)                             160                                                                              MUSIGHNPF                                                                               :EQQAELRPGKGNTEQPKQVTSSTSET*                                                                          (SEQ ID NO: 373)                             161                                                                              MUSIGHNPE                                                                               :EQQAELKPGKGNTEQPKLITSSTSET*                                                                          (SEQ ID NO: 374)                             162                                                                              A27635   :TGQAELRPGKGAPEQGKKGKSSTSDR*                                                                          (SEQ ID NO: 375)                             163                                                                              MUSIGHXW :QYQAELRPGKGTPRQQKKGKSSTSES*                                                                          (SEQ ID NO: 376)                             164                                                                              MUSIGHIZA                                                                               :QQQAVLRHGKGTHGQEKKGKSSTSES*                                                                          (SEQ ID NO: 377)                             165                                                                              MUSIGHEM :QQQTKLGPGRGTPGQGRKGKSSTSGS*                                                                          (SEQ ID NO: 378)                             166                                                                              MUSIGHRH :EQQAELRAGKGTPGQEKKGKSSVYFA*                                                                          (SEQ ID NO: 379)                             167                                                                              HV00$MOUSE                                                                              :EQQAELKAGKGTPGQQKQGESTRSET*                                                                          (SEQ ID NO: 380)                             168                                                                              N$1F9H   :QQKAELAASKGTPGQEKKGRSSTSES*                                                                          (SEQ ID NO: 381)                             169                                                                              MUSIGHZAD                                                                               :QQQTELRPGKGTPGQEKRGKSSNLRL*                                                                          (SEQ ID NO: 382)                             170                                                                              B30515   :EKVGGLQGSSFDPGKASKGTSQRAET*                                                                          (SEQ ID NO: 383)                             171                                                                              MUSIGHEB :EQQADLKLGKGNPEQPKLATPSTSET*                                                                          (SEQ ID NO: 384)                             172                                                                              E27889   :EQVGGLKPGKGTPDKSDVKDNAKSET*                                                                          (SEQ ID NO: 385)                             173                                                                              MUSIGHAAC                                                                               :DQQPDLKPSSGSPGHPSKSTSKTTET*                                                                          (SEQ ID NO: 386)                             174                                                                              HV61$MOUSE                                                                              :DQQPDLKPSSGSPGNPSKSTSKTTET*                                                                          (SEQ ID NO: 387)                             175                                                                              MUSIGVHR2                                                                               :DQQPDLKPSSGSPGNPSKSTSKTAET*                                                                          (SEQ ID NO: 388)                             176                                                                              PL0100   :DQQPGLKPSSGSPGNPSKSTSKTTET*                                                                          (SEQ ID NO: 389)                             177                                                                              MUSIGHAAO                                                                               :DQQPGLKPSSGSPGNPSKNTSKTTET*                                                                          (SEQ ID NO: 390)                             178                                                                              MUSIGHGA6                                                                               :DQQPGLKPSSGSPGDPSKTTSKTTET*                                                                          (SEQ ID NO: 391)                             179                                                                              MUSIGHJY :DQQPGLKPSSGSPGNPSKTTSKTTET*                                                                          (SEQ ID NO: 392)                             180                                                                              MUSIGHGA1                                                                               :DHQPGLKPSSGSPGNPSKNTSKTTET*                                                                          (SEQ ID NO: 393)                             181                                                                              MUSIGHXX :DQQPGLKPSSGSPGNPSRSTSKTTET*                                                                          (SEQ ID NO: 394)                             182                                                                              HV62$MOUSE                                                                              :DQQPGLKPSAGSPGNPSKSTSKTAET*                                                                          (SEQ ID NO: 395)                             183                                                                              MUSIGHAAGA                                                                              :EQQPGLKPSSGSPGNPSKSTSKTSET*                                                                          (SEQ ID NO: 396)                             184                                                                              MUSIGHGA5                                                                               :DQQPGLKPSSGSPGNPSKNTSKTIET*                                                                          (SEQ ID NO: 397)                             185                                                                              MUSIGHGA4                                                                               :DQQPGLKPSSGSPGDPSKNTSKTPET*                                                                          (SEQ ID NO: 398)                             186                                                                              MUSIGHAGI                                                                               :EQQPSLKPSSGSPGNPSKSTSKTTET*                                                                          (SEQ ID NO: 399)                             187                                                                              PL0102   :DQQPGLKPSSGSPGNPSKNTSETTET*                                                                          (SEQ ID NO: 400)                             188                                                                              HV46$MOUSE                                                                              :DQQPGLKPSSGSPGNPSKNTSETTZT*                                                                          (SEQ ID NO: 401)                             189                                                                              MUSIGHZT :EQQPSLKPSSGSPGNPSKSTSKTSET*                                                                          (SEQ ID NO: 402)                             190                                                                              MUSIGHAGD                                                                               :EQQPSLKPSSGSPGNPSKSTSRTTET*                                                                          (SEQ ID NO: 403)                             191                                                                              MUSIGHAGO                                                                               :EQQPSLKPSSGSPGNPSKSTSKTAET*                                                                          (SEQ ID NO: 404)                             192                                                                              MUSIGAM32                                                                               :DQQPDLKPSSGFPGNPSKSTSKTTET*                                                                          (SEQ ID NO: 405)                             193                                                                              MUSIGHAFX                                                                               :EQQPSLKPSSGSPGKPSKSTSKTNET*                                                                          (SEQ ID NO: 406)                             194                                                                              MUSIGHAGE                                                                               :EQQPSLKPSSGSPGNPSKSTFKTSET*                                                                          (SEQ ID NO: 407)                             195                                                                              MUSIGHAGB                                                                               :EQQPSLKPSSGSPGNPSKSTSTTSET*                                                                          (SEQ ID NO: 408)                             196                                                                              MUSIGHAGC                                                                               :EQQLSLKPSSGSPGNPSKSTSKTTET*                                                                          (SEQ ID NO: 409)                             197                                                                              MUSIGHAAM                                                                               :QQQPGLKPSFGPPGKPSQSTSKTTET*                                                                          (SEQ ID NO: 410)                             198                                                                              HV43$MOUSE                                                                              :QQKPGLAPSSGSPGKSTKSNSKQTDT*                                                                          (SEQ ID NO: 411)                             199                                                                              MUSIGMUV1                                                                               :QQKPGLAPSSGSPGKSAKSNSKQTDT*                                                                          (SEQ ID NO: 412)                             200                                                                              MUSIGHAEI                                                                               :QQKPGLAPSSGSPGKSAMSNSKQTDT*                                                                          (SEQ ID NO: 413)                             201                                                                              MUSIGHBP :QQKPGLAPSSGSPGKSAISNSKQTDT*                                                                          (SEQ ID NO: 414)                             202                                                                              MUSIGHZZA                                                                               :QQKPGLQPSSGSPGKAAISNSKQSNT*                                                                          (SEQ ID NO: 415)                             203                                                                              MUSIGMUV2                                                                               :QQKPGLQPSSGSPGKAAISNSKQANT*                                                                          (SEQ ID NO: 416)                             204                                                                              A32456   :QQKPVLAPSSGSPGKSAMSNSKQIDT*                                                                          (SEQ ID NO: 417)                             205                                                                              MUSIGHMB :QQKPSLQPSSDSPGKAAMSNSKQADT*                                                                          (SEQ ID NO: 418)                             __________________________________________________________________________     HUMAN HEAVY CHAIN SURFACE PATCHES                                              __________________________________________________________________________     1 HUMIGHVS :ERVGDLEPGRGIPGKAPKGDSKKIET*                                                                          (SEQ ID NO: 419)                             2 HUMIGHVR :ERVGDLEPERGIPGKAPKGDSKKIET*                                                                          (SEQ ID NO: 420)                             3 H36005   :EQVGGLKPGRGTPGKAPKGDSKKTET*                                                                          (SEQ ID NO: 421)                             4 PL0122   :EQVGGLQPGKGTSGKASKGDSKKTET*                                                                          (SEQ ID NO: 422)                             5 HV3D$HUMAN                                                                              :EQLGGLQPGRGTPGKBSKGDSKRAET*                                                                          (SEQ ID NO: 423)                             6 HUMIGHAT :EQLGGLQPGRGTPGKDSKGNSKRAET*                                                                          (SEQ ID NO: 424)                             7 B34964   :EQLGGLQPGRGTPGKDSRGNSKRAET*                                                                          (SEQ ID NO: 425)                             8 A34964   :EQVGGLQPGRGTPGKDSKGNSKRAET*                                                                          (SEQ ID NO: 426)                             9 PL0123   :EQVGGLQPGRGTPGKDSKGNAKRAET*                                                                          (SEQ ID NO: 427)                             10                                                                               HV3F$HUMAN                                                                              :EQVGGLQPGRGTPGKDSKGDSRRAET*                                                                          (SEQ ID NO: 428)                             11                                                                               JL0048   :EQVGGLQPGRGTPGKDSKGNSRRAET*                                                                          (SEQ ID NO: 429)                             12                                                                               HV3B$HUMAN                                                                              :QQVGGLEPGRGTPGKDSKGBSKRAET*                                                                          (SEQ ID NO: 430)                             13                                                                               HUMIGHBV :EQLGDLQPGRGTPGKASKGNSKRAET*                                                                          (SEQ ID NO: 431)                             14                                                                               HV3E$HUMAN                                                                              :EQVGGLQPGRGTTGKDSKGDSKRAET*                                                                          (SEQ ID NO: 432)                             15                                                                               PL0116   :QQVGGVQPGRGTPGKDSKGNSKRAET*                                                                          (SEQ ID NO: 433)                             16                                                                               KV3K$HUMAN                                                                              :QQVGGVQPGRGIPGKDSKGNSKRPET*                                                                          (SEQ ID NO: 434)                             17                                                                               N$2FB4H  :EQVGGVQPGRGIPGKDSKGDSKRPET*                                                                          (SEQ ID NO: 435)                             18                                                                               HV3I$HUMAN                                                                              :QQVGGVQPGRGTPGKDSNGDSKRPET*                                                                          (SEQ ID NO: 436)                             19                                                                               HV3J$HUMAN                                                                              :QKVGGVQPGRGTPGKDSKGNSKRTET*                                                                          (SEQ ID NO: 437)                             20                                                                               HV3G$HUMAN                                                                              :QEVGGVZPGRGTPGKBSKGBSKRAET*                                                                          (SEQ ID NO: 438)                             21                                                                               HV3M$HUMAN                                                                              :EQLGGLQPGRGTPGKDSNGDSKQAZT*                                                                          (SEQ ID NO: 439)                             22                                                                               HV3O$HUMAN                                                                              :EQLGGLQPGRGSPGKDTNGDSKEAZT*                                                                          (SEQ ID NO: 440)                             23                                                                               HV3N$HUMAN                                                                              :AQLGGLQPGRGTPGKDSNGDSKQAZS*                                                                          (SEQ ID NO: 441)                             24                                                                               HV3R$HUMAN                                                                              :EQLGGLQPGRGTPGKVSQGDSKQAZT*                                                                          (SEQ ID NO: 442)                             25                                                                               HV3P$HUMAN                                                                              :EQVGGLQPGRGTPGKVSQGDSKEPZT*                                                                          (SEQ ID NO: 443)                             26                                                                               HUMIGHCV :EQLGGLQPERGTPGKESKGNSMRAET*                                                                          (SEQ ID NO: 444)                             27                                                                               HV3T$HUMAN                                                                              :EQVGDLQPGRGBPGKDSKGNAKRVET*                                                                          (SEQ ID NO: 445)                             28                                                                               HV3U$HUMAN                                                                              :EQVGDLQPGRGNPGKDSKGNAQRPET*                                                                          (SEQ ID NO: 446)                             29                                                                               PL0098   :QQVGGVQPGRGTLGKDSKGNSKRAET*                                                                          (SEQ ID NO: 447)                             30                                                                               HV3H$HUMAN                                                                              :QZVGGAZPGRGSPGKASKGBSKRAET*                                                                          (SEQ ID NO: 448)                             31                                                                               HV3A$HUMAN                                                                              :QQVGGLKPGRGSPGKDSKGNAQRTZT*                                                                          (SEQ ID NO: 449)                             32                                                                               HV3S$HUMAN                                                                              :DQVGGLKPGRGTPGKNSNGDSKTPZT*                                                                          (SEQ ID NO: 450)                             33                                                                               HUMIGHAN :EQLGGLQPGRGTSREDSKGNSKRAET*                                                                          (SEQ ID NO: 451)                             34                                                                               HV3Q$HUMAN                                                                              :EQVGALQPGRGTPGKDSQADSKEAZT*                                                                          (SEQ ID NO: 452)                             35                                                                               A36040   :EQLGGLQPGRGTPGK----VEGSVET*                                                                          (SEQ ID NO: 453)                             36                                                                               HUMIGHAN :EQVGAFQPGRGNSGKASKGDSKRPDT*                                                                          (SEQ ID NO: 454)                             37                                                                               HUMIGHAO :EQVGAFQPGKGNSGKASKGDSKRPDT*                                                                          (SEQ ID NO: 455)                             38                                                                               HUMIGHAR :EQVGAFQPGKGNSGKASKGDSNRPDT*                                                                          (SEQ ID NO: 456)                             39                                                                               HV3L$HUMAN                                                                              :QQVGGVQAGRANPGKDSRGISKRTET*                                                                          (SEQ ID NO: 457)                             40                                                                               HV1A$HUMAN                                                                              :QQVAEVKPGKGTPGQQKQGESTRSET*                                                                          (SEQ ID NO: 458)                             41                                                                               A32483   :QQVAEVKPGKGTPGQQKQGTSTRSET*                                                                          (SEQ ID NO: 459)                             42                                                                               HUMIGHAY :QQVAEVKPGKGTPGQQKQGTSARSET*                                                                          (SEQ ID NO: 460)                             43                                                                               HUMIGHCU :QQVAEVKPGKGTPGQQKQGTSIRSDT*                                                                          (SEQ ID NO: 461)                             44                                                                               HUMIGHBS :QQVAEVKPGKGTPGQEKQGTSIRSDT*                                                                          (SEQ ID NO: 462)                             45                                                                               HUMIGVHLS                                                                               :QQVAEVKPGKGTPGQQNQGTSTRSDT*                                                                          (SEQ ID NO: 463)                             46                                                                               HUMIGHBX :QQVGEVKPGRGTPGQQKQDTSTRSDT*                                                                          (SEQ ID NO: 464)                             47                                                                               HV1C$HUMAN                                                                              :QQVAEVKPGRGTPGHPRQGASFRSDS*                                                                          (SEQ ID NO: 465)                             48                                                                               H34964   :QQVSELKPGKGTPGQQGTGTSVKAET*                                                                          (SEQ ID NO: 466)                             49                                                                               HUMIGHCY :EQVAEVKPGKGSPGKPSQGKSIKAST*                                                                          (SEQ ID NO: 467)                             50                                                                               PL0119   :EQVAEVKPGRGSPGKPSQGKSIKAST*                                                                          (SEQ ID NO: 468)                             51                                                                               HV1F$HUMAN                                                                              :QQVAEVKPGRGDPGRPRQASSTISAT*                                                                          (SEQ ID NO: 469)                             52                                                                               D34964   :EQVAEVPQGKGRPGKSLQGKSLKAST*                                                                          (SEQ ID NO: 470)                             53                                                                               HV1D$HUMAN                                                                              :QQMAEVKPGRGTPGKPGVVPSFFSET*                                                                          (SEQ ID NO: 471)                             54                                                                               HV1E$HUMAN                                                                              :QQVAEVKPGRGTPGRYIWEPSFFNEG*                                                                          (SEQ ID NO: 472)                             55                                                                               JL0047   :QQQAGLKPSSGSPGKPSKSTSKTAAT*                                                                          (SEQ ID NO: 473)                             56                                                                               HUMIGHBW :QQQPGLKPSSGSPGKPSKSTSKTAAT*                                                                          (SEQ ID NO: 474)                             57                                                                               E34964   :QQQPGLKPSSGSPGKPSKSTSNTAAT*                                                                          (SEQ ID NO: 475)                             58                                                                               HUMIGHCW :QQQPGLKPSSGSAGKPSKSTSKTAAT*                                                                          (SEQ ID NO: 476)                             59                                                                               HV2F$HUMAN                                                                              :RQQPGLKPSSGPPGKPSRGTSRSAAT*                                                                          (SEQ ID NO: 477)                             60                                                                               HV2I$HUMAN                                                                              :QQQAGLKPSSGSPGRTSKSTSKTAAT*                                                                          (SEQ ID NO: 478)                             61                                                                               HV2G$HUMAN                                                                              :QQEPGLRPSSGTPGRTPRSTSKTAAT*                                                                          (SEQ ID NO: 479)                             62                                                                               N$3FABH  :XQEPGLRPSSGSPGRTPRSTSKTAAT*                                                                          (SEQ ID NO: 480)                             63                                                                               PS0091   :QQQPGLKPSSGSPSRVSKSTSKTPET*                                                                          (SEQ ID NO: 481)                             64                                                                               HUMIGHDA :QHQAGLKRSSGPPGKPSTSTSKTAAT*                                                                          (SEQ ID NO: 482)                             65                                                                               A26555   :ZQESGLKPTSGSPGKPSKSRSKAADA*                                                                          (SEQ ID NO: 483)                             66                                                                               HV2E$HUMAN                                                                              :QTKPTLKPTTGSPGRPSKSTSKDPVT*                                                                          (SEQ ID NO: 484)                             67                                                                               HV2D$HUMAN                                                                              :QTKPTLKPTTGSPGKPSRSTSRDPVS*                                                                          (SEQ ID NO: 485)                             68                                                                               A36005   :ETRPALKPTTGSPGKTSKTTSKDPVT*                                                                          (SEQ ID NO: 486)                             69                                                                               HV2H$HUMAN                                                                              :QNRPALKATTGSPGKTSETTSKDPAT*                                                                          (SEQ ID NO: 487)                             70                                                                               HV2A$HUMAN                                                                              :QTTPALKPKTGSPGKTSRTDSKNPVT*                                                                          (SEQ ID NO: 488)                             71                                                                               HV2C$HUMAN                                                                              :QTRPALRPTTGSPGEASETTSKGPGT*                                                                          (SEQ ID NO: 489)                             72                                                                               HV2B$HUMAN                                                                              :QTRPALKPTTGSPGKTSETTSRDTAY*                                                                          (SEQ ID NO: 490)                             73                                                                               JL0049   :LEGVQLWGGRGISRKYAKGNGKRDES*                                                                          (SEQ ID NO: 491)                             __________________________________________________________________________

EXAMPLE 2 Detailed Description of Method for Constructing Three-Dimensional Model of Antibody Variable Region

The references cited in the text below are listed at the end of this Example.

The first antibody Fab structure was determined in 1972. Since then, no more than about twelve Fab structures have been published, a number that represents a very small fraction of the total antibody repertoire (>10⁸ antibodies). To understand the molecular basis of this antibody diversity will require knowledge of either a large number of x-ray structures, or the rules by which combining site topography is governed. The development of such prediction rules has now reached the point where variable regions of antibodies can be modelled to an accuracy approaching that of the medium resolution x-ray structure.

The interaction of an antibody with its cognate antigen is one of the most widely accepted paradigms of molecular recognition. To understand the antibody-antigen interaction in atomic detail requires knowledge of the three-dimensional structure of antibodies and of their antigen complexes. Traditionally such information has come from x-ray crystallographic studies (see Davies et al. for review (Davies et al., 1988)).

The modelling of antibody combining sites was first attempted by Padlan & Davies (Padlan et al., 1976) at a time when very few antibody structures were known. Nonetheless, Padlan and colleagues recognized that the key lay in high structural homology that existed within the β-sheet framework regions of different antibody variable domains. The antigen combining site is formed by the juxtaposition of six interstrand loops, or CDRs (Complementarity Determining Regions) (Kabat et al., 1987), on this framework. If the framework could be modelled by homology then it might be possible to model the CDRs in the same way. Padlan and Davies (Padlan et al., 1976) reasoned that CDR length was the important determinant of backbone conformation though the number of antibody structures was insufficient to thoroughly test this maximum overlap procedure (MOP). This notion was not picked up again until the early 1980's when Pedersen and Rees proposed a similar approach to modelling antibody combining sites based on a more extensive analysis of antibody structures (de la Pas et al., 1986).

Those essentially knowledge-based procedures are best exemplified for antibodies by the work of Chothia & Lesk (Chothia et al., 1986) who, in 1986, extended and modified the MOP procedure by introducing the concept of "key" residues. These residues allow the further subdivision of CDRs of the same length into "canonical" structures which differ in having residues at specified positions that, through packing, hydrogen bonding or the ability to assume unusual values of the torsion angels φ, ψ and ω, determine the precise CDR conformation (Chothia et al., 1989). Similar knowledge-based methods have been proposed for predicting loop conformations in general (Thornton et al., 1988; Tramontano et al., 1989). These methods rely on the crystallographic database of protein structures. However, none of the above knowledge-based methods has been totally successful. In particular, the MOP or canonical structure approaches have succeeded in modelling only five of the six CDRs. This stems from the fact that the third CDR of the heavy chain, H3, is more variable in sequence, length and structure than any of the other CDRs.

To deal with this problem several groups have attempted to use ab initio methods to model the combining site (Bruccoleri and Karplus, 1987). The requirement with such methods is that the total allowable conformational space accessible to a particular CDR is sampled. Typical of purely geometric approaches is that of Go & Sheraga (Go and Sheraga, 1970) and more recently Palmer & Sheraga (Palmer and Sheraga, 1991), where the problem is reduced to one in which the central region of the polypeptide backbone, having characteristic bond length and bond angles, is constructed between the end points of the loop (CDR if an antibody loop) by a "chain closure" algorithm. In a modification of this algorithm, Bruccoleri & Karplus (Bruccoleri and Karplus, 1987) introduced an energy minimization procedure which greatly expanded the domain of conformational space searched during the chain closure procedure. This modification is incorporated into the conformational search program CONGEN (Bruccoleri and Karplus, 1987), which also allows the user to choose any set of standard bond length and bond angels such as the CHARMM (Brooks et al., 1983) standard geometry parameter sets. Other approaches such as minimization (Moult and James, 1986), or molecular dynamics (Fine et al., 1986) either fail to saturate conformational space or are unable to deal with the problem of long CDRs. Whichever of the ab initio methods is employed however, the problem is one of defining the selection criteria in such a way as to allow the unambiguous identification of the correct structure (in this context correct is defined by reference to an appropriate X-ray structure) within the ensemble of candidates, for every CDR. To date this has not been possible.

Recently a more holistic approach has been taken to the modelling of CDRs which combines the advantages of knowledge-based and ab initio methods in a single algorithm known as CAMAL (Combined Algorithm for Modelling Antibody Loops) (Martin et al., 1989; Martin et al., 1991). Previously this algorithm has been used to model individual CDRs in the presence of the crystal structure conformations of the other five. As is demonstrated below, CAMAL is able to predict the backbone conformations of all six CDRs of the antibody combining site to an accuracy approaching that of medium resolution x-ray structures. In addition the algorithm includes a procedure for selecting and fitting together the light and heavy chain framework regions prior to generation of CDR conformations, thus making possible the prediction of the entire variable region. Furthermore a new Monte Carlo (MC) simulated annealing method has been developed for the determination of sidechain conformations.

the Framework Region

Antibody framework regions consist of conserved β-strands that form the β-barrel structure characteristic of immunoglobulin V-type regions. In the procedure described here each V-region is built from a database of known antibody structures, using sequence homology for selection of the light (L) and heavy (H) chain V-domains. The two domains are then paired by least squares fitting on the most conserved strands of the antibody β-barrel (Table 2 and FIGS. 5 & 6). The strand orientations were determined by analyzing the barrels of known antibody crystal structures. Eight antibodies were analyzed using a multiple structure fitting program as follows. Seven structures were fitted onto one of the set selected at random and mean coordinates were calculated. All eight structures were then fitted onto these mean coordinates and new mean coordinates determined. This procedure was iterated until the mean coordinate set converged (5-10 cycles). The variance for the mean coordinates at each barrel point (N,Cα,C) was calculated. In FIG. 5 this variance is plotted against the projected positions of these points onto the conjugate axis of the barrel.

Strand 8 and all but two residues of strand 7 in both light and heavy chains were eliminated as they showed deviations greater than 3σ (standard deviation units) from the mean coordinates. These two strands comprised the takeoff points of CDR H3, and suggests that any knowledge-based prediction of CDR H3 would have to account not only for sequence and length variation in the CDR itself, but also for the position of the participating strands. The remaining mean coordinates were used as a scaffold onto which the L and H chains were fitted. Strands 7 and 8 in the final framework were obtained from the database structure used in the construction. The framework strands are marked + in the multialignment in Table 2.

The sidechains were then replaced using a . `maximum overlap` method, in which sidechain templates were fitted on backbone atoms with the sidechain torsion angles being adjusted to match those of equivalent torsions in the parent sidechain.

The Combining Site

The procedure for predicting the structure of combining sites combines a database search with a conformational search procedure. The architecture of the program suite to perform this task is outlined in FIG. 7.

The database search utilizes distance constraints for each of the six CDR loops determined from known antibody structures. These constraints were determined by calculating Cα--Cα distances within known loops and using a search range of x+3.5σ (the mean ±3.5 standard deviation units). A database containing all the proteins in the Brookhaven Protein Databank (Bernstein et al., 1977) is then searched for fragments which satisfy the constraints for a loop of the required length. The middle section of the loop is then deleted and reconstructed using the conformational search program CONGEN (Bruccoleri and Karplus, 1987). For loops of six or seven residues, the structure database appears to saturate the conformational space available to the backbone adequately and only sidechains are built by conformational search. Loops shorter than six residues are built by conformational search alone since this is computationally feasible and the number of loops selected from the database becomes unacceptably large as loop length decreases.

When modelling a complete combining site, loops of 6 or more residues are modelled individually with the other loops absent. If the loops are built consecutively, small errors can accumulate leading to a poor result (Martin, 1990). All the loop conformations are then evaluated using a solvent modified potential, which excludes the attractive van der Waals and electrostatic terms of the non-bonded energy function contained within the GROMOS (Åqvist et al., 1985) potential. The lowest five energy conformations are selected and filtered using a "structurally determining residue" algorithm (FILTER), based on backbone torsion angles observed in the original database loops. Since the database search is not used for the shortest loops of 5 residues or fewer, the FILTER algorithm cannot be used. Energy is thus the only available selection criterion and the short loops are built last, in the presence of the longer loops.

Side Chains

The determination of sidechain positions was previously done using the iterative sidechain determination algorithm described by Bruccoleri et al. (Bruccoleri and Karplus, 1987). Unfortunately the CHARMM (Brooks et al., 1983) force field fails to select the correct conformations of exposed hydrophobic sidechains. There is no penalty for having an exposed uncharged atom, without solvent present. CONGEN is also unable to saturate the conformational space for a large number of sidechains (more than 6 residues).

Recently Lee et al. (Lee and Levitt, 1991; Lee and Subbiah, 1991) has proposed a method for searching conformational space for a large number of sidechains using MC simulated annealing. A simple energy function is used for the evaluation of conformations generated by a biased random walk: ##EQU1## Where the first term is a simple Lennard-Jones potential which evaluates the non-bonded contacts between the atoms in a given molecule, the second term is a simple torsional term which only applies to C--C bonds. The torsional term biases the function towards 60° rotamers. ε_(o) and κ_(o) are constants. The metropolis function: ##EQU2## is used to evaluate the energy function. Any move which results in a decrease in energy is accepted, and any move which results in a positive δE is only accepted with the probability P. This simple method can be used to search the large conformational space defined by a set of torsion angles in amino-acid sidechains, and find or define the global minimum which exist for a set of sidechains. T is the simulation temperature.

When searching sidechain conformations using this method the simulation system usually gets trapped in an energetic minima well before the global minimum is encountered, at a high temperature, without the solution space having been searched sufficiently. This problem can be solved by truncating the Lennard-Jones potential, thus allowing atoms to pass through each other. In reality this function would converge towards infinity when the distance r between the atoms approaches zero.

The evaluation of sidechain conformations generated is done solely on the basis of energy, for internal (core) residues, since good van der Waal's interactions are considered to be equal to a good packing of the sidechains. The situation becomes more complicated when trying to predict the conformation of surface residues. The lowest van der Waal's interaction is obtained by a combination of sidechain conformations which minimize the overlap of atoms, this means that the lowest energy is obtained with extended conformations of sidechains, without considering good packing of sidechains.

Using the fact that hydrophobic, bulky residues will be shielded by the hydrophilic sidechains, and will be buried in the surface, it is possible to generate a simple function which will evaluate these macroscopic observations. These functions can either be implemented in the objective evaluation function of the Monte Carlo simulation, or as is done here, added as a post processing step. Including an accessibility/hydrophobicity term in the evaluation function would slow down the calculation considerably, hence the term has been added as a post processing function. The function used is a sum of the product of relative exposed surface area multiplied by the residual hydrophobicities. The hydrophobicities used are taken from Cornette et al. (Cornette et al., 1987). ##EQU3## n is the number of sidechains reconstructed. The surface area is calculated using the tesselated icosahedron approach (Chau and Dean, 1987), which is not very precise (0.1 percent), but is able to evaluate a large number of conformations. The function is evaluated for the final 2,000 conformations and the lowest value conformation selected as the best.

Using this simple approach it is possible to integrate over a large phase space with many degrees of freedom, and get a complete sampling of the space.

Predicted Structures of an Anti-hapten, Anti-peptide and Two Anti-protein Antibodies

In the following section the predicted structures of four different antibody F_(V) regions are presented and analyzed. The antibodies are:

Gloop-2 (Darsley and Rees, 1985), an anti-lysozyme antibody whose Fab structure was previously determined and which was used as a learning exercise during the development of CAMAL.

D1.3 (Amir et al., 1986), an anti-lysozyme antibody whose uncomplexed F_(V) coordinates were supplied by R. Poljak et al. after the model coordinates had been deposited.

36-71 (Rose et al., 1990), an anti-phenylarsonate antibody whose Fab structure was carried out by D. R. Rose, et al., and whose coordinates were obtained after the model coordinates had been deposited.

3D6 (Grunow et al., 1988), an anti-protein (GP41 of HIV) antibody whose Fab structure was carried out by D. Carter et al. (Carter, 1991) and whose coordinates were obtained after the model coordinates had been deposited. For this antibody, the model was generated using the canonical loop method of Chothia & Lesk (Chothia et al., 1989; Chothia et al., 1986) for CDRs L1, L2, H1 and H2, while L3 and H3, which cannot be modelled using canonical structures, were constructed using CAMAL.

All four models were subjected to both restrained and unrestrained energy minimization using the DISCOVER (TM Biosym Technology) potential with 300 cycles of steepest descents, followed by conjugate gradient minimization until convergence to within 0.01 Kcal occurred.

The resolution and R-factors of the x-ray structures are given in Table 3 together with the parent frameworks selected in building the models. The structures and models were compared by global fits of the loops. The β-barrel strands 1 to 6, as described above, were least squares fitted and the RMS deviation was then calculated over the loops. The backbone (N,Cα,C) RMS values for fitting model and crystal structure frameworks were between 0.4 and 0.9 Å, illustrating the conservation of the core β-barrel. Using all eight strands RMS deviations between 0.6 and 1.2 Å were observed.

Global fits (Table 4) give a more realistic measure of the accuracy of the model than a local least-squares fit over the loops since they account for the overall positioning of the loops in the context of the F_(V) structure. Local fits, which give lower RMS deviations, are also shown in Table 4. Differences between local and global RMS deviations arise from differences in V_(H) /V_(L) domain packing and differences in loop `take off` angles and positions.

Table 5 shows the canonical loops selected from modelling 3D6. Backbone structures of the modelled CDRs, superimposed on the x-ray structures after global fitting are shown in FIG. 8. General features and points of interest for each of the six CDRs are discussed below.

Analysis of the CDR Regions

During the comparison of CDR conformations in the V-region models and the x-ray Fab structures it was observed that at certain positions in a CDR, the peptide backbone may adopt either of two conformations by undergoing a "peptide flip" (1,4 shift). This phenomenon is also seen in type 2 β-turns (Paul et al., 1990). Dynamics simulations of β-turns show that the transformation energy between φ1=-00, ψ1=-30, φ2=-90, ψ2=0 and φ1=-00, ψ1=120, φ2=90, ψ2=0 has a maximum value of 5 kcal (Paul et al., 1990). This is low enough to allow selection of either conformation. The peptide flip is observed within several canonical classes (as described by Chothia et al. (Chothia et al., 1989)) and the hydrogen bonding pattern used to determine the conformation of a canonical class does not disallow the peptide flip. Any modelling procedure should therefore take these, or any other multiple conformations, into consideration where the transformation energies are sufficiently low to permit population of the different conformational forms. Table 6 shows an example of the "peptide-flip" phenomenon from the crystallographic database of antibody structures. It should be noted that a single crystal structure will not show multiple conformations since the crystallization will `freeze out` one of the conformations. During the modelling procedure the two populations of conformers are easily extracted from a set of ab initio generated loops, by using a torsional clustering algorithm.

CDR-L1

In Gloop-2 and D1.3, all five low energy conformations were very similar with RMS deviations differing by less than 0.25 Å (backbone) and 0.35 Å (all atoms). The FILTER algorithm was unable to distinguish between the conformations and the lowest energy structure was selected.

Although CDR-L1 of 3D6 was originally built using the canonical loop from HyHEL-10, the mid-section was rebuilt by conformational search, for the following reason. HyHEL-10 and REI CDR-L1 loops are placed in the same canonical ensemble (Chothia et al., 1989) although they contain a 1-4 shift (peptide flip) relative to one another between the fifth and eighth residues of the loop (residues 28-31) (see Table 6).

36-71 shows the same 1-4 shift between the model and crystal structure CDRs. Both crystal structure and model were compared with other loops of the same canonical class as defined by Chothia et al. (Chothia et al., 1989). It was found that the hydrogen bonding pattern which determines the conformation was conserved.

CDR-L2

CDR-L2 of D1.3 has two adjacent threonines (49, 50) which in the x-ray structure are packed against the tyrosine at the fourth position of CDR-H3, thus minimizing the exposed hydrophobic sidechains. In the unminimized model the threonine sidechains are exposed to the solvent, but after energy minimization, this packing is observed.

CDR-L3

In Gloop-2, D1.3 and 36-71 the proline at the seventh position in the loop is correctly predicted in the cis conformation. It has previously been suggested that the conformation of CDR-L3 is dictated by the presence of a proline in position 8 or 9 (Chothia et al., 1989) within the loop. 3D6 does not have a proline in either position. Only 7 out of 290 CDR-L3 sequences (Kabat et al., 1987) lack a proline at both positions and in all of the published x-ray structures this proline is present. This is an example of a situation where either a new canonical class may need to be defined or where the canonical rule breaks down altogether, and an alternative method must be employed.

The 3D6 L3 loop is 7 residues in length and was built using database loops alone where conformational space is saturated by means of fragments selected from the crystallographic database (Global RMS: 2.01 Å, N,Cα,C), and by using CAMAL (Construction: Q[Q(YNS)Y]S, Global RMS: 1.97 Å, N,Cα,C). The similarity of the structures generated by the two procedures illustrates the utility of the database search and suggests that, for shorter loops it is capable of saturating the available conformational space.

CDR-H1

Using the Kabat and Wu definition of CDR-H1 places this loop as an extension of the β-sheet. The extended nature of this stretch of peptide limits its conformational flexibility and CDR-H1 is generally modelled accurately (Martin et al., 1989; Chothia et al., 1989).

In Gloop-2 and D1.3, the Phe or Tyr sidechain at the second position in the loop is poorly placed and packs against Leu at the penultimate position in HFR1 (see Table 2). 36-71 has a well-placed Asn at this position, rather than the more common bulky hydrophobic sidechain.

CDR-H2

CDR-H2 of 36-71 is similar in sequence to F19.9 (Strong et al., 1991), (36-71: YNNPGNGYIA (SEQ ID NO:492); F19.9: YINPGKGYLS (SEQ ID NO:493)). While the structurally determining residues specified by Chothia and Lesk (Chothia et al., 1989) are conserved, the backbone conformations are different: F19.9 has a bulge at the --PGN-- Gly, compared with 36-71, giving the loop a `kink` in the middle. The model of 36-71 shows a 1-4 shift, though the sidechains are still well placed.

In Gloop-2, the all atom RMS deviation is poor (3.00 Å) when compared with the P2₁ crystal structure, owing to rotations of the Phe at position 3 in the loop and Tyr at position 10 by approximately 120° about the χ₂ torsion angle. Gloop-2 has been solved in two different crystal forms, P2₁ and P1 Jeffrey, 1989). When compared with the P1 structure, the sidechains are placed almost perfectly and the all atom RMS (global fit) drops to 2.23 Å.

This concerted sidechain motion between crystal forms illustrates the effects of crystallization conditions on surface sidechain placement. Even though surface sidechains may show low temperature factors indicating low mobility in the crystal, their mobility in solution may be high. In the Gloop-2 P1 structure, the mean sidechain temperature factor for the F_(V) domain is 13.46 (σ=8.20) while the sidechains of these two residues of H2 show mean temperature factors of 5.56 (σ=0.68) for the Phe at position 3 and 7.10 (σ=1.73) for the Tyr at position 10.

CDR-H3

CDR-H3 is the most variable of the six CDR's with all lengths up to 21 residues being represented in Kabat et al., (Kabat et al., 1987). This extreme variability results from V-D-J splicing (Schilling et al., 1980) and has always been a problem when attempting to model antibodies. Such loops may be divided into short (up to 7 residues), medium (up to 14 residues) and long (15 or more residues). Using the CAMAL procedure, short and medium CDR-H3's can be modelled as accurately as other CDR's of similar lengths. Although long CDR-H3's are more difficult and cannot, at present, be built to the same accuracy, the chain trace is still correct.

It is unlikely that the longer loops consist of `pure` loops (i.e., all random coil or turn). In crystal structures of antibodies with medium to long CDR-H3 loops (McPC603 (Rudikoff et al., 1981): 11 amino acids (aa); KOL (Marquart et al., 1980): 17 aa; F19.9 (Lascombe et al., 1989): 15 aa) the loops consist of a disordered β-sheet extension from the β-barrel core and a 5-8 residue random coil/turn connecting these two strands.

To determine the nature of medium to long loops (>8 residues) which satisfy the CDR-H3 constraints, a complete search of the Protein Databank for loops of length 8-20 residues, was performed using the inter-Cα distance constraints determined from known antibody crystal structures for CDR-H3. The resulting loops were then analyzed using the DSSP (Kabsch and Sander, 1983) program, which is able to assign secondary structure to polypeptide structures. The amount of secondary structure for each length of loop was calculated, and it was observed that for loops longer than 12 residues the amount of secondary structure within each of the classes described in DSSP was constant. The number of loops selected is also constant (approximately 150 loops) for loops longer than 12 residues. A closer inspection of each of the length ensembles shows indeed that the loops are the same between the groups.

This analysis shows that, like the long CDR-H3 crystal structures, the selected fragments consist of β-strands connected by 5-8 residue loops. For loops above 12-13 residues in length, the same loops are selected, but with extensions to the β-strands. This is called the "sliding-ladder" effect. In addition, the maximum size of a random coil or turn fragment in any of the structures contained in the Protein Databank tends not to exceed 8 residues, as determined by DSSP. This implies that the conformational space of longer loops is not saturated by the database and, although it is unlikely that long loops in antibodies will differ significantly from long loops in other structures, confidence in the prediction must be correspondingly reduced.

By how much is the usefulness of the CAMAL algorithm reduced by this observation?

The frequency of occurrence of different CDR-H3 lengths in antibody sequences described by Kabat et al. (Kabat et al., 1987) was analyzed. FIG. 10 shows that more than 85% of H3 loops have lengths between 4 and 14 residues which can be modelled accurately by the CAMAL algorithm.

CDR-H3 of D1.3 is of average length (8 residues), though no loops of this length are seen in the available antibody structures. The crystal structure coordinate set showed an RMS of 1.9 Å compared with the model.

The 36-71 loop is 12 residues long. The conformation is correctly predicted as a short loop connecting an extension of the β-sheet.

The 3D6 H3 loop is 17 residues long. While KOL (Marquart et al., 1980) has the same length it has only one residue in common with 3D6 and only one conservative mutation. There is thus no reason to believe that the conformations would be similar. The final predicted conformation of 3D6 is an extended β-sheet, as in the crystal structure. The difference between the predicted and the crystal structure of 3D6-H3 is due to a twist of 5°-7° in the extended β-sheet loop (see FIGS. 9A-9D). Such a twist has also been observed for complexed and uncomplexed antibodies by Wilson et al. (Wilson and others). This suggests that long CDR-H3 loops may be flexible and actively involved in antigen binding.

The Complete Variable Region

Prediction of the strand positions and V_(L) -V_(H) orientation in the framework β-barrel was exact for all of the four antibodies. The backbone (N,Cα,C) RMS deviations from the crystal structures were between 0.56 and 0.86 Å, despite the fact that, in all cases the V_(L) and V_(H) regions of a particular model were derived from different antibody structures. This suggests that this method will do well in procedures such as humanization (Gorman et al., 1991), where correct framework positioning is important. The backbones of all six CDRs in all four antibodies are essentially correctly predicted, as shown in FIG. 8. There are two important points to make about these predictions. First, the position of each CDR on its framework barrel is correct. Thus, CDR-framework interactions can be confidently monitored. The only deviation from the x-ray structure is CDR-H3 of antibody 3D6 which has been previously discussed. Second, the all atom RMS deviation between models and x-ray structures is dominated by sidechain positions. In most instances this deviation is due to a small number of incorrectly positioned, exposed sidechains (for example, in D1.3 the only sidechains which are incorrectly predicted are Tyr 9 of L1, Trp 4 of L3, Tyr 2 of H1 and Tyr 4 of H3). Since each CDR is constructed in the absence of other CDRs, the force field may choose a rotamer which is 120° away from that found in the crystal structure. This effect has also been observed by Lee et al. (Lee and Levitt, 1991).

Conclusion

For antibodies having CDR H3 regions of 14 residues or less the complete variable domain can be modelled to an accuracy approaching that of medium resolution x-ray structures. For antibodies with longer H3 loops the CAMAL algorithm is likely to need an additional procedure in which molecular dynamics simulations are also incorporated.

The canonical approach of Chothia et al. appears to work well (at least in modelling backbones) where it may be applied and may be used successfully in combination with the CAMAL procedure.

One important observation that has emerged from these studies is that a given loop can exist in several conformations. In particular, this seems likely for CDR-L1 and, to a lesser extent, CDR-L3 and longer CDR-H3's. A simple combinatorial calculation shows that, if each of these three loops can exist in three separate conformations, a given combining site can have 3³ =27 different topographies. Clearly, this would explain the origins of cross reactivity and would allow for induced fit of antigens.

    TABLE 2       - Alignment of antibody sequences used in the modeling. `*` indicates      CDR regions; `+` indicates β-strand regions used in the fitting for      modelling frameworks.       Nomenclature for β-barrel strands is (H or L - Chain) - FR(Framewor      k region)-(Strand number), thus for example strand one of the heavy      chain becomes HFR1.        SEQ.       Antibody ID. No. sequence L-chain       ##STR1##       ##STR2##       ##STR3##       ##STR4##       ##STR5##       ##STR6##       ##STR7##       ##STR8##       ##STR9##       ##STR10##       ##STR11##       ##STR12##       ##STR13##       ##STR14##       ##STR15##        Seq.       Antibody ID. No. sequence L-chain       ##STR16##       ##STR17##       ##STR18##       ##STR19##       ##STR20##       ##STR21##       ##STR22##       ##STR23##       ##STR24##       ##STR25##       ##STR26##       ##STR27##       ##STR28##       ##STR29##       ##STR30##       ##STR31##       ##STR32##

                  TABLE 3                                                          ______________________________________                                         Details of the antibody crystal structures against which the                   models were compared and the parent frameworks used to build                   the models. Resolution data for D1.3 has not yet been published.                                  Framework Model                                             Antibody                                                                               Resolution R-factor  Light  Heavy                                      ______________________________________                                         Gloop-2 2.80       21.2      REI    HyHEL-5                                    D1.3    --         --        REI    NEW                                        36-71   1.90       20.9      Gloop2 NEW                                        3D6     2.70       17.7      REI    KOL                                        ______________________________________                                    

                                      TABLE 4                                      __________________________________________________________________________     Sequence and conformational search construction scheme for each of the 24      CRDs, [ ] = construction area,                                                 ( ) = Chain closure, all sidechains are constructed. RMS(Root Mean             Square) difference between model and crystal loop coordinates.                 The RMS values are a global fit calculated by least-squares fitting the        conserved core of the two structures upon each other and                       calculating the RMS over the loops. The total RMS of the frameworks            (N,Ca,C) is 0.81, 0.60, 0.86 and 0.56 respectivly                                                      SEQ.                                                                           ID.                                                                               RMS local (Å) RMS global (Å)                Antibody                                                                            CDR sequence       No.                                                                               Cα                                                                          N,Cα,C                                                                        All CG                                                                              All MC                                                                              Cα                                                                          N,Cα,C                                                                        All                                                                                 All                  __________________________________________________________________________                                                               MC                   Gloop-2                                                                             L1  RAS[Q(EIS)G]YLS                                                                               494                                                                               0.73                                                                              0.71 2.05 1.96 0.86                                                                              0.87 2.09 2.12                 D1.3     RAS[G(NIH)N]YLA                                                                               495                                                                               2.29                                                                              1.93 4.34 3.94 2.72                                                                              2.43 4.59 4.32                 36-71    RAS[Q(DIN)N]FLN                                                                               496                                                                               2.71                                                                              2.43 4.80 4.59 3.51                                                                              3.31 5.19 5.67                 3D6      RAS[Q(SIG)N]NLH                                                                               497                                                                               0.51                                                                              0.54 2.48 1.82 0.61                                                                              0.78 2.68 1.96                 Gloop-2                                                                             L2  AASTLDS        498                                                                               0.25                                                                              0.23 0.80 1.00 0.66                                                                              0.68 1.10 1.10                 D1.3     Y[T(TTL)A]D    499                                                                               0.67                                                                              0.73 1.80 1.40 0.99                                                                              1.02 2.01 1.98                 36-71    F[T(SRS)Q]S    500                                                                               0.64                                                                              0.66 2.34 2.23 0.73                                                                              0.72 2.43 2.40                 3D6      KASSLES        501                                                                               0.41                                                                              0.42 1.37 1.20 0.83                                                                              0.86 1.73 1.80                 Gloop-2                                                                             L3  LQ[Y(LSY)P]LT  502                                                                               0.56                                                                              0.52 1.73 1.60 0.75                                                                              0.74 2.00 1.90                 D1.3     QH[F(WST)P]RT  503                                                                               1.41                                                                              1.35 2.69 2.98 1.76                                                                              1.79 3.46 3.20                 36-71    QQ[G(NAL)P]RT  504                                                                               1.09                                                                              1.00 2.26 2.10 1.46                                                                              1.36 2.36 2.28                 3D6      Q[Q(YNS)Y]S    505                                                                               1.46                                                                              1.68 3.64 3.90 2.31                                                                              1.97 3.96 3.90                 Gloop-2                                                                             H1  [T(FGI)T]      506                                                                               0.60                                                                              0.70 2.00 1.60 1.03                                                                              1.01 2.04 2.00                 D1.3     [G(YGV)N]      507                                                                               0.44                                                                              0.62 2.33 2.00 0.65                                                                              0.90 3.24 2.98                 36-71    [S(NGI)N]      508                                                                               0.90                                                                              0.63 2.22 1.96 1.04                                                                              0.97 2.51 2.23                 3D6      DYAMH          509                                                                               0.67                                                                              0.77 1.52 1.11 0.61                                                                              0.72 1.59 1.20                 Gloop-2                                                                             H2  EI[F(PGN)S]KYY 510                                                                               0.63                                                                              0.64 1.63 1.70 1.20                                                                              0.94 2.23 2.10                 D1.3     MI[W(GDG)N]TD  511                                                                               0.42                                                                              0.42 1.55 1.40 0.87                                                                              0.85 1.66 1.80                 36-71    YNN[P(GNG)Y]IA 512                                                                               0.64                                                                              0.78 2.01 2.20 1.47                                                                              1.41 1.79 1.98                 3D6      ISWDSSSIG      513                                                                               0.45                                                                              0.52 2.35 2.03 0.95                                                                              0.89 2.85 2.10                 Gloop-2                                                                             H3  [R(EIR)Y]      514                                                                               0.66                                                                              0.89 3.44 3.90 0.87                                                                              1.07 3.66 4.15                 D1.3     ER[D(YRL)D]Y   515                                                                               0.38                                                                              0.53 1.66 1.20 1.25                                                                              0.01 1.96 1.33                 36-71    SEYY[G(GSY)K]FDY                                                                              516                                                                               1.95                                                                              1.75 4.40 4.00 2.65                                                                              2.53 4.60 4.09                 3D6      GRDYY[D(SGG)YF]TVAFDI                                                                         517                                                                               3.66                                                                              3.42 5.93 4.01 4.30                                                                              3.95 6.30 6.20                 __________________________________________________________________________

                  TABLE 5                                                          ______________________________________                                         Canonical loops selected for the model of                                      3D6(taken from Chothia et al (1989)).                                          Loop  Canonical Sequence         Seq. ID. No.                                  ______________________________________                                         L1    HyHEL-10  R A S Q S I S R W L A                                                                           518                                                 (3D6)     R A S Q S I G N N L H                                                                           497                                           L2    REI       E A S N D L A    519                                                 (3D6)     K A S S L E S    501                                           H1    McPC603   D F Y M E        520                                                 (3D6)     D Y A M H        509                                           H2    KOL       I I W D D G S D Q                                                                               521                                                 (3D6)     I S W D S S S I G                                                                               513                                           ______________________________________                                    

                                      TABLE 6                                      __________________________________________________________________________     Backbone φ and ψ angles of residues in CDR-L1 from                     HyHEL-10 and REI classified in the same canonical group by Chothia             et al (1989). The residues exhibiting a peptide flip are indicated by a        *.                                                                             Residue Number                                                                            24   25    26   27    28*   29*                                     __________________________________________________________________________     REI   Sequence                                                                            Q    A     S    Q     S     I                                             φ/ψ                                                                         --/138                                                                              -103/157                                                                             -96/7                                                                               -158/142                                                                             -40/108                                                                              -112/9                                  HyHEL-10                                                                             Sequence                                                                            R    A     S    Q     S     I                                             φ/ψ                                                                         --/108                                                                              -85/135                                                                              -88/64                                                                              172/160                                                                              -64/-38                                                                              9/63                                    __________________________________________________________________________     Residue Number                                                                            30*  31*   32   33    32                                            __________________________________________________________________________     REI   Sequence                                                                            I    K     Y    L     N     Seq. ID. NO. 522                              φ/ψ                                                                         79/-77                                                                              -146/21                                                                              -104/89                                                                             -143/133                                                                             -144/--                                       HyHEL-10                                                                             Sequence                                                                            G    N     N    L     H     Seq. ID. NO: 518                              φ/ψ                                                                         -63/107                                                                             85/-15                                                                               -105/72                                                                             -129/118                                                                             -126/--                                       __________________________________________________________________________

M. J. Darsley, P de al Paz, D. C. Phillips and A. R. Rees in Methodological Surveys in Biochemistry and Analysis, pages 63-68, Volume 15, 1985, Plenum Press (Eds. E. Reid, G. M. W. Cook and D. J. Morre), Presented at the Ninth International Subcellular Methodology Forum, Sep. 3-6, 1984, Guildford, UK.

Amit, A. G., Mariuzza, R. A., Phillips, S. E. V. and Poljak, R. J. (1986). The Three-dimensional Structures of an Antigen-antibody Complex at 2.8 Å Resolution. Science 233, pp. 747-753.

Åqvist, J., van Gunsieren, W., Leifonmark, M. and Tapia, O. (1985), J. Mol. Biol. 183, pp. 461-477.

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Brooks, B., Bruccoleri, R., Olaison, B., Statcs, D., Swaminathan, S. and Karplus, M. (1983), J. Comp. Chem. 4, pp. 187-217.

Bruccoleri, R. E. and Karplus, M. (1987), Prediction of the Folding of Short Polypeptide Segments by Uniform Conformational Sampling. Biopolymers 26, pp. 137-168.

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Chau, P. and Dean P. (1987). Molecular Recognition: 3d Surface Structure Comparison by Gnomonic Projection. J. Mol. Graph. 5, pp. 97-100.

Chothia, C., Lesk, A., Levitt, M., Amit, A., Mariuzza, R., Phillips, S. and Poljak, R. (1986). The Predicted Structure of Immunoglobulin D1.3 and its Comparison with the Crystal Structure. Science 233, pp. 755-758.

Chothia, C., Lesk, A. M., Tramontano, A., Levitt, M., Smith-Gill, S. J., Air, G., Sheriff, S., Padlan, E. A., Davies, D. R., Tulip, W. R., Colman, P. M., Alzri, P. M. and Poljak, R. J. (1989). Conformations of Immunoglobulin Hypervariable Regions. Nature (London) 342, pp. 877-883.

Cornette, J. L., Cease, K. B., Margalit, H., Spouge, J. L., Berzofsky, J. A. and Delisi, C. (1987). Hydrophobicity Scales and Computational Techniques for Detecting Amphipatic Structures in Proteins. Journal of Molecular Biology 195.3, pp. 659-685.

Darsley, M. and Rees, A. (1985), EMBO J. 4, pp. 383-392.

Davies, D., Sheriff, S. and Padlan, E. (1988). Antibody Antigen Complexes. J. Biol. Chem. 263, pp. 10541-10544.

de la Paz, P., Sutton, B., Darsly, M. and Rees, A. (1986). Modelling of the Combining Sites of Three Anti-lysozyme Monoclonal Antibodies and of the Complex Between One of the Antibodies and Its Epitope. EMBO J. 5, pp. 415-425.

Fine, R., Wang, H., Shenkin, P., Yarmush, D. and Levinthar, C. (1986). Predicting Antibody Hypervariable Loop Conformations ii: Minimization and Molecular Dynamics Studies of McPC603 from Many Randomly Generated Loop Conformations. Proteins: Struct., Funct., Genet. 1, pp. 342-362.

Go, N. and Sheraga, H. (1970). Ring Closure and Local Conformational Deformations of Chain Molecules. Macromolecules 3, pp. 178-187.

Gorman, S., Clark, M., Rutledge, E., Cobbold, S. and Waldman, H. (1991). Reshaping a Therapeutic CD4 Antibody. Proc. Natl. Acad. Sci. U.S.A. 88, pp. 4181-4185.

Grunow, R., Jahn, S., Porstman, T., Kiessig, T., Steinkeller, H., Steindl, F., Mattanovich, D., Gurtler, L., Deinhardt, F., Katinger, H. and von R., B. (1988). The High Efficiency, Human B Cell Immortalizing Heteromyeloma cb-f7. J. Immunol. Meth. 106, pp. 257-265.

Jeffrey, P. (1989). The Structure and Specificity of Immunoglobulins. D. Phil. Thesis, University of Oxford.

Lascombe, M., Alzari, P., Boulot, G., Salujian, P., Tougard, P., Berek, C., Haba, S., Rosen, E., Nisonof, A. and Poljak, R. (1989). Three-dimensional Structure of Fab r19.9, A Monoclonal Murine Antibody Specific for the pazobenzenearsonate Group. Proc. Natl. Acad. Sci. U.S.A. 86, p. 607.

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Lee, C. and Subbiah, S. (1991). Prediction of Protein Side-chain Conformation by Packing Optimization. Journal of Molecular Biology 217.2, pp. 373-388.

Marquart, M., Deisenhofer, J. and Huber, R. (1980), Crystallographic Refinement and Atomic Models of the Intact Immunoglobulin Molecule KOL and Its Antigen-binding Fragment at 3.0 Å and 1.9 Å Resolution. J. Mol. Biol. 141, pp. 369-391.

Martin, A. C. R. (1990). Molecular Modelling of Antibody Combining Sites. D. Phil. Thesis, University of Oxford.

Martin, A. C. R., Cheetham, J. C. and Rees, A. R. (1989). Modelling Antibody Hypervariable Loops: A Combined Algorithm. Proc. Natl. Acad. Sci. U.S.A. 86, pp. 9268-9272.

Martin, A. C. R., Cheetham, J. C. and Rees, A. R. (1991). Modelling Antibody Hypervariable Loops using a `Combined Algorithm`. Meth. Enz. In press.

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Padlan, E., Davies, D., Pecht, I., Givol, D. and Wright, C. (1976). Model Building Studies of Antigen-binding Sites: The Hapten-Binding Site of MOPC-315. Cold Spring Harbor Quant. Symp. Biochem. 41, pp. 627-637.

Palmer, K. and Sheraga, J. (1991). Standard-geometry Chains Fitted to X-ray Deviated Structures: Validation of the Rigid-geometry Approximation. I. Chain Closure through a Limited Search of Loop Conformations. J. Comp. Chem. 12, pp. 505-526.

Paul, P., Burney, P., Campbell, M. and Odguthorpe, D. (1990). The Conformational Preferences of γ-lactam and Its Role in Constraining Peptide Structure. J. Comp.-aided. Mol. Des. 4, pp. 239-253.

Rose, D. R., Strong, R. K., Margolis, M. N., Gefter, M. L. and Petsko, G. A. (1990). Crystal Structure of the Antigen-binding Fragment of the Murine Anti-arsonate Monoclonal Antibody 36-71 at 2.9 Å Resolution. Proc. Natl. Acad. Sci. U.S.A. 87, pp. 338-342.

Rudikoff, S., Satow, Y., Padlan, E. A., Davies, D. R. and Potter, M. (1981). Kappa Chain Structure from a Crystallized Murine Fab': The Role of the Joining Segment in Hapten Binding. Mol. Immunol. 18, pp. 705-711.

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Wilson, I. et al., Presented at Structure and Function Meeting in Honour of Sir David Phillips, 1-3 Jul., 1991, Oxford, UK.

    __________________________________________________________________________     SEQUENCE LISTING                                                               (1) GENERAL INFORMATION:                                                       (iii) NUMBER OF SEQUENCES: 522                                                 (2) INFORMATION FOR SEQ ID NO:1:                                               (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 109 amino acids                                                    (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:1:                                        AspIleGlnMetThrGlnSerProSerSerLeuSerAlaSerLeuGly                               151015                                                                         GluArgValSerLeuThrCysArgAlaSerGlnGluIleSerGlyTyr                               202530                                                                         LeuSerTrpLeuGlnGlnLysProAspGlyThrIleLysArgLeuIle                               354045                                                                         TyrAlaAlaSerThrLeuAspSerGlyValProLysArgPheSerGly                               505560                                                                         ArgArgSerGlySerAspTyrSerLeuThrIleSerSerLeuGluSer                               65707580                                                                       GluAspPheAlaAspTyrTyrCysLeuGlnTyrLeuSerTyrProLeu                               859095                                                                         ThrPheGlyAlaGlyThrLysLeuGluLeuLysArgAla                                        100105                                                                         (2) INFORMATION FOR SEQ ID NO:2:                                               (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 109 amino acids                                                    (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:2:                                        AspIleGlnMetThrGlnSerProAlaSerLeuSerAlaSerValGly                               151015                                                                         GluThrValThrIleThrCysArgAlaSerGlyAsnIleHisAsnTyr                               202530                                                                         LeuAlaTrpTyrGlnGlnLysGlnGlyLysSerProGlnLeuLeuVal                               354045                                                                         TyrTyrThrThrThrLeuAlaAspGlyValProSerArgPheSerGly                               505560                                                                         SerGlySerGlyThrGlnTyrSerLeuLysIleAsnSerLeuGlnPro                               65707580                                                                       GluAspPheGlySerTyrTyrCysGlnHisPheTrpSerThrProArg                               859095                                                                         ThrPheGlyGlyGlyThrLysLeuGluIleLysArgArg                                        100105                                                                         (2) INFORMATION FOR SEQ ID NO:3:                                               (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 107 amino acids                                                    (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:3:                                        AspIleValLeuThrGlnSerProAlaIleMetSerAlaSerProGly                               151015                                                                         GluLysValThrMetThrCysSerAlaSerSerSerValAsnTyrMet                               202530                                                                         TyrTrpTyrGlnGlnLysSerGlyThrSerProLysArgTrpIleTyr                               354045                                                                         AspThrSerLysLeuAlaSerGlyValProValArgPheSerGlySer                               505560                                                                         GlySerGlyThrSerTyrSerLeuThrIleSerSerMetGluThrGlu                               65707580                                                                       AspAlaAlaGluTyrTyrCysGlnGlnTrpGlyArgAsnProThrPhe                               859095                                                                         GlyGlyGlyThrLysLeuGluIleLysArgAla                                              100105                                                                         (2) INFORMATION FOR SEQ ID NO:4:                                               (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 109 amino acids                                                    (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:4:                                        AspIleValLeuThrGlnSerProAlaThrLeuSerValThrProGly                               151015                                                                         AsnSerValSerLeuSerCysArgAlaSerGlnSerIleGlyAsnAsn                               202530                                                                         LeuHisTrpTyrGlnGlnLysSerHisGluSerProArgLeuLeuIle                               354045                                                                         LysTyrAlaSerGlnSerIleSerGlyIleProSerArgPheSerGly                               505560                                                                         SerGlySerGlyThrAspPheThrLeuSerIleAsnSerValGluThr                               65707580                                                                       GluAspPheGlyMetTyrPheCysGlnGlnSerAsnSerTrpProTyr                               859095                                                                         ThrPheGlyGlyGlyThrLysLeuGluIleLysArgAla                                        100105                                                                         (2) INFORMATION FOR SEQ ID NO:5:                                               (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 108 amino acids                                                    (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:5:                                        GluIleValLeuThrGlnSerProAlaIleThrAlaAlaSerLeuGly                               151015                                                                         GlnLysValThrIleThrCysSerAlaSerSerSerValSerSerLeu                               202530                                                                         HisTrpTyrGlnGlnLysSerGlyThrSerProLysProTrpIleTyr                               354045                                                                         GluIleSerLysLeuAlaSerGlyValProAlaArgPheSerGlySer                               505560                                                                         GlySerGlyThrSerTyrSerLeuThrIleAsnThrMetGluAlaGlu                               65707580                                                                       AspAlaAlaIleTyrTyrCysGlnGlnTrpThrTyrProLeuIleThr                               859095                                                                         PheGlyAlaGlyThrLysLeuGluLeuLysArgAla                                           100105                                                                         (2) INFORMATION FOR SEQ ID NO:6:                                               (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 112 amino acids                                                    (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:6:                                        GluSerValLeuThrGlnProProSerAlaSerGlyThrProGlyGln                               151015                                                                         ArgValThrIleSerCysThrGlyThrSerSerAsnIleGlySerIle                               202530                                                                         ThrValAsnTrpTyrGlnGlnLeuProGlyMetAlaProLysLeuLeu                               354045                                                                         IleTyrArgAspAlaMetArgProSerGlyValProThrArgPheSer                               505560                                                                         GlySerLysSerGlyThrSerAlaSerLeuAlaIleSerGlyLeuGlu                               65707580                                                                       AlaGluAspGluSerAspTyrTyrCysAlaSerTrpAsnSerSerAsp                               859095                                                                         AsnSerTyrValPheGlyThrGlyThrLysValThrValLeuGlyGln                               100105110                                                                      (2) INFORMATION FOR SEQ ID NO:7:                                               (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 115 amino acids                                                    (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:7:                                        AspIleValMetThrGlnSerProSerSerLeuSerValSerAlaGly                               151015                                                                         GluArgValThrMetSerCysLysSerSerGlnSerLeuLeuAsnSer                               202530                                                                         GlyAsnGlnLysAsnPheLeuAlaTrpTyrGlnGlnLysProGlyGln                               354045                                                                         ProProLysLeuLeuIleTyrGlyAlaSerThrArgGluSerGlyVal                               505560                                                                         ProAspArgPheThrGlySerGlySerGlyThrAspPheThrLeuThr                               65707580                                                                       IleSerSerValGlnAlaGluAspLeuAlaValTyrTyrCysGlnAsn                               859095                                                                         AspHisSerTyrProLeuThrPheGlyAlaGlyThrLysLeuGluIle                               100105110                                                                      LysArgAla                                                                      115                                                                            (2) INFORMATION FOR SEQ ID NO:8:                                               (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 103 amino acids                                                    (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:8:                                        SerValLeuThrGlnProProSerValSerGlyAlaProGlyGlnArg                               151015                                                                         ValThrIleSerCysThrGlySerSerSerAsnIleGlyAlaGlyAsn                               202530                                                                         HisValLysTrpTyrGlnGlnLeuProGlyThrAlaProLysLeuLeu                               354045                                                                         IlePheHisAsnAsnAlaArgPheSerValSerLysSerGlySerSer                               505560                                                                         AlaThrLeuAlaIleThrGlyLeuGlnAlaGluAspGluAlaAspTyr                               65707580                                                                       TyrCysGlnSerTyrAspArgSerLeuArgValPheGlyGlyGlyThr                               859095                                                                         LysLeuThrValLeuArgGln                                                          100                                                                            (2) INFORMATION FOR SEQ ID NO:9:                                               (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 114 amino acids                                                    (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:9:                                        AspValValMetThrGlnThrProLeuSerLeuProValSerLeuGly                               151015                                                                         AspGlnAlaSerIleSerCysArgSerSerGlnSerLeuValHisSer                               202530                                                                         GlnGlyAsnThrTyrLeuArgTrpTyrLeuGlnLysProGlyGlnSer                               354045                                                                         ProLysValLeuIleTyrLysValSerAsnArgPheSerGlyValPro                               505560                                                                         AsnArgPheSerGlySerGlySerGlyThrAspPheThrLeuLysIle                               65707580                                                                       SerArgValGluAlaGluAspLeuGlyValTyrPheCysSerGlnSer                               859095                                                                         ThrHisValProTrpThrPheGlyGlyGlyThrLysLeuGluIleLys                               100105110                                                                      ArgAla                                                                         (2) INFORMATION FOR SEQ ID NO:10:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 109 amino acids                                                    (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:10:                                       AspIleGlnMetThrGlnThrThrSerSerLeuSerAlaSerLeuGly                               151015                                                                         AspArgValThrIleSerCysArgAlaSerGlnAspIleSerAsnTyr                               202530                                                                         LeuAsnTrpTyrGlnGlnLysProAspGlyThrValLysLeuLeuVal                               354045                                                                         TyrTyrThrSerArgLeuHisSerGlyValProSerArgPheSerGly                               505560                                                                         SerGlySerGlyThrAspTyrSerLeuThrIleSerAsnLeuGluHis                               65707580                                                                       GluAspIleAlaThrTyrPheCysGlnGlnGlySerThrThrProArg                               859095                                                                         ThrPheGlyGlyGlyThrLysLeuGluIleLysArgArg                                        100105                                                                         (2) INFORMATION FOR SEQ ID NO:11:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 109 amino acids                                                    (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:11:                                       AspIleGlnMetThrGlnIleProSerSerLeuSerAlaSerLeuGly                               151015                                                                         AspArgValSerIleSerCysArgAlaSerGlnAspIleAsnAsnPhe                               202530                                                                         LeuAsnTrpTyrGlnGlnLysProAspGlyThrIleLysLeuLeuIle                               354045                                                                         TyrPheThrSerArgSerGlnSerGlyValProSerArgPheSerGly                               505560                                                                         SerGlySerGlyThrAspTyrSerLeuThrIleSerAsnLeuGluGln                               65707580                                                                       GluAspIleAlaThrTyrPheCysGlnGlnGlyAsnAlaLeuProArg                               859095                                                                         ThrPheGlyGlyGlyThrLysLeuGluIleLysArgAla                                        100105                                                                         (2) INFORMATION FOR SEQ ID NO:12:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 107 amino acids                                                    (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:12:                                       AspIleGlnMetThrGlnSerProSerThrLeuSerAlaSerValGly                               151015                                                                         AspArgValThrIleThrCysArgAlaSerGlnSerIleSerArgTrp                               202530                                                                         LeuAlaTrpTyrGlnGlnLysProGlyLysValProLysLeuLeuIle                               354045                                                                         TyrLysAlaSerSerLeuGluSerGlyValProSerArgPheSerGly                               505560                                                                         SerGlySerGlyThrGluPheThrLeuThrIleSerSerLeuGlnPro                               65707580                                                                       AspAspPheAlaThrTyrTyrCysGlnGlnTyrAsnSerTyrSerPhe                               859095                                                                         GlyProGlyThrLysValAspIleLysArgThr                                              100105                                                                         (2) INFORMATION FOR SEQ ID NO:13:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 104 amino acids                                                    (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:13:                                       GlnValGlnLeuGlnGlnSerGlyThrGluLeuAlaArgProGlyAla                               151015                                                                         SerValArgLeuSerCysLysAlaSerGlyTyrThrPheThrThrPhe                               202530                                                                         GlyIleThrTrpValLysGlnArgThrGlyGlnGlyLeuGluTrpIle                               354045                                                                         GlyGluIlePheProGlyAsnSerLysThrTyrTyrAlaGluArgPhe                               505560                                                                         LysGlyLysAlaThrLeuThrAlaAspLysSerSerThrThrAlaTyr                               65707580                                                                       MetGlnLeuSerSerLeuThrSerGluAspSerAlaValTyrPheCys                               859095                                                                         AlaArgGluIleArgTyrTrpGly                                                       100                                                                            (2) INFORMATION FOR SEQ ID NO:14:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 107 amino acids                                                    (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:14:                                       GlnValGlnLeuLysGluSerGlyProGlyLeuValAlaProSerGln                               151015                                                                         SerLeuSerIleThrCysThrValSerGlyPheSerLeuThrGlyTyr                               202530                                                                         GlyValAsnTrpValArgGlnProProGlyLysGlyLeuGluTrpLeu                               354045                                                                         GlyMetIleTrpGlyAspGlyAsnThrAspTyrAsnSerAlaLeuLys                               505560                                                                         SerArgLeuSerIleSerLysAspAsnSerLysSerGlnValPheLeu                               65707580                                                                       LysMetAsnSerLeuHisThrAspAspThrAlaArgTyrTyrCysAla                               859095                                                                         ArgGluArgAspTyrArgLeuAspTyrTrpGly                                              100105                                                                         (2) INFORMATION FOR SEQ ID NO:15:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 106 amino acids                                                    (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:15:                                       ValGlnLeuGlnGlnSerGlyAlaGluLeuMetLysProGlyAlaSer                               151015                                                                         ValLysIleSerCysLysAlaSerGlyTyrThrPheSerAspTyrTrp                               202530                                                                         IleGluTrpValLysGlnArgProGlyHisGlyLeuGluTrpIleGly                               354045                                                                         GluIleLeuProGlySerGlySerThrAsnTyrHisGluArgPheLys                               505560                                                                         GlyLysAlaThrPheThrAlaAspThrSerSerSerThrAlaTyrMet                               65707580                                                                       GlnLeuAsnSerLeuThrSerGluAspSerGlyValTyrTyrCysLeu                               859095                                                                         HisGlyAsnTyrAspPheAspGlyTrpGly                                                 100105                                                                         (2) INFORMATION FOR SEQ ID NO:16:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 104 amino acids                                                    (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:16:                                       AspValGlnLeuGlnGluSerGlyProSerLeuValLysProSerGln                               151015                                                                         ThrLeuSerLeuThrCysSerValThrGlyAspSerIleThrSerAsp                               202530                                                                         TyrTrpSerTrpIleArgLysPheProGlyAsnArgLeuGluTyrMet                               354045                                                                         GlyTyrValSerTyrSerGlySerThrTyrTyrAsnProSerLeuLys                               505560                                                                         SerArgIleSerIleThrArgAspThrSerLysAsnGlnTyrTyrLeu                               65707580                                                                       AspLeuAsnSerValThrThrGluAspThrAlaThrTyrTyrCysAla                               859095                                                                         AsnTrpAspGlyAspTyrTrpGly                                                       100                                                                            (2) INFORMATION FOR SEQ ID NO:17:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 109 amino acids                                                    (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:17:                                       GluValLysLeuLeuGluSerGlyGlyGlyLeuValGlnProGlyGly                               151015                                                                         SerLeuLysLeuSerCysAlaAlaSerGlyPheAspPheSerLysTyr                               202530                                                                         TrpMetSerTrpValArgGlnAlaProGlyLysGlyLeuGluTrpIle                               354045                                                                         GlyGluIleHisProAspSerGlyThrIleAsnTyrThrProSerLeu                               505560                                                                         LysAspLysPheIleIleSerArgAspAsnAlaLysAsnSerLeuTyr                               65707580                                                                       LeuGlnMetSerLysValArgSerGluAspThrAlaLeuTyrTyrCys                               859095                                                                         AlaArgLeuHisTyrTyrGlyTyrAsnAlaTyrTrpGly                                        100105                                                                         (2) INFORMATION FOR SEQ ID NO:18:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 117 amino acids                                                    (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:18:                                       GluValGlnLeuValGlnSerGlyGlyGlyValValGlnProGlyArg                               151015                                                                         SerLeuArgLeuSerCysSerSerSerGlyPheIlePheSerSerTyr                               202530                                                                         AlaMetTyrTrpValArgGlnAlaProGlyLysGlyLeuGluTrpVal                               354045                                                                         AlaIleIleTrpAspAspGlySerAspGlnHisTyrAlaAspSerVal                               505560                                                                         LysGlyArgPheThrIleSerArgAsnAspSerLysAsnThrLeuPhe                               65707580                                                                       LeuGlnMetAspSerLeuArgProGluAspThrGlyValTyrPheCys                               859095                                                                         AlaArgAspGlyGlyHisGlyPheCysSerSerAlaSerCysPheGly                               100105110                                                                      ProAspTyrTrpGly                                                                115                                                                            (2) INFORMATION FOR SEQ ID NO:19:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 113 amino acids                                                    (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:19:                                       GluValLysLeuValGluSerGlyGlyGlyLeuValGlnProGlyGly                               151015                                                                         SerLeuArgLeuSerCysAlaThrSerGlyPheThrPheSerAspPhe                               202530                                                                         TyrMetGluTrpValArgGlnProProGlyLysArgLeuGluTrpIle                               354045                                                                         AlaAlaSerArgAsnLysGlyAsnLysTyrThrThrGluTyrSerAla                               505560                                                                         SerValLysGlyArgPheIleValSerArgAspThrSerGlnSerIle                               65707580                                                                       LeuTyrLeuGlnMetAsnAlaLeuArgAlaGluAspThrAlaIleTyr                               859095                                                                         TyrCysAlaArgAsnTyrTyrGlySerThrTrpTyrPheAspValTrp                               100105110                                                                      Gly                                                                            (2) INFORMATION FOR SEQ ID NO:20:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 107 amino acids                                                    (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:20:                                       ValGlnLeuGluGlnSerGlyProGlyLeuValArgProSerGlnThr                               151015                                                                         LeuSerLeuThrCysThrValSerGlyThrSerPheAspAspTyrTyr                               202530                                                                         SerThrTrpValArgGlnProProGlyArgGlyLeuGluTrpIleGly                               354045                                                                         TyrValPheTyrHisGlyThrSerAspThrAspThrProLeuArgSer                               505560                                                                         ArgValThrMetLeuValAsnThrSerLysAsnGlnPheSerLeuArg                               65707580                                                                       LeuSerSerValThrAlaAlaAspThrAlaValTyrTyrCysAlaArg                               859095                                                                         AsnLeuIleAlaGlyCysIleAspValTrpGly                                              100105                                                                         (2) INFORMATION FOR SEQ ID NO:21:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 109 amino acids                                                    (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:21:                                       GluValLysLeuAspGluThrGlyGlyGlyLeuValGlnProGlyArg                               151015                                                                         ProMetLysLeuSerCysValAlaSerGlyPheThrPheSerAspTyr                               202530                                                                         TrpMetAsnTrpValArgGlnSerProGluLysGlyLeuGluTrpVal                               354045                                                                         AlaGlnIleArgAsnLysProTyrAsnTyrGluThrTyrTyrSerAsp                               505560                                                                         SerValLysGlyArgPheThrIleSerArgAspAspSerLysSerSer                               65707580                                                                       ValTyrLeuGlnMetAsnAsnLeuArgValGluAspMetGlyIleTyr                               859095                                                                         TyrCysThrGlySerTyrTyrGlyMetAspTyrTrpGly                                        100105                                                                         (2) INFORMATION FOR SEQ ID NO:22:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 115 amino acids                                                    (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:22:                                       GlnValGlnLeuLysGluSerGlyAlaGluLeuValAlaAlaSerSer                               151015                                                                         SerValLysMetSerCysLysAlaSerGlyTyrThrPheThrSerTyr                               202530                                                                         GlyValAsnTrpValLysGlnArgProGlyGlnGlyLeuGluTrpIle                               354045                                                                         GlyTyrIleAsnProGlyLysGlyTyrLeuSerTyrAsnGluLysPhe                               505560                                                                         LysGlyLysThrThrLeuThrValAspArgSerSerSerThrAlaTyr                               65707580                                                                       MetGlnLeuArgSerLeuThrSerGluAspSerAlaValTyrPheCys                               859095                                                                         AlaArgSerPheTyrGlyGlySerAspLeuAlaValTyrTyrPheAsp                               100105110                                                                      SerTrpGly                                                                      115                                                                            (2) INFORMATION FOR SEQ ID NO:23:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 112 amino acids                                                    (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:23:                                       GluValGlnLeuGlnGlnSerGlyValGluLeuValArgAlaGlySer                               151015                                                                         SerValLysMetSerCysLysAlaSerGlyTyrThrPheThrSerAsn                               202530                                                                         GlyIleAsnTrpValLysGlnArgProGlyGlnGlyLeuGluTrpIle                               354045                                                                         GlyTyrAsnAsnProGlyAsnGlyTyrIleAlaTyrAsnGluLysPhe                               505560                                                                         LysGlyLysThrThrLeuThrValAspLysSerSerSerThrAlaTyr                               65707580                                                                       MetGlnLeuArgSerLeuThrSerGluAspSerAlaValTyrPheCys                               859095                                                                         AlaArgSerGluTyrTyrGlyGlySerTyrLysPheAspTyrTrpGly                               100105110                                                                      (2) INFORMATION FOR SEQ ID NO:24:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 117 amino acids                                                    (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:24:                                       GluValGlnLeuValGluSerGlyGlyGlyLeuValGlnProGlyArg                               151015                                                                         SerLeuArgLeuSerCysAlaAlaSerGlyPheThrPheAsnAspTyr                               202530                                                                         AlaMetHisTrpValArgGlnAlaProGlyLysGlyLeuGluTrpVal                               354045                                                                         SerGlyIleSerTrpAspSerSerSerIleGlyTyrAlaAspSerVal                               505560                                                                         LysGlyArgPheThrIleSerArgAspAsnAlaLysAsnSerLeuTyr                               65707580                                                                       LeuGlnMetAsnSerLeuArgAlaGluAspMetAlaLeuTyrTyrCys                               859095                                                                         ValLysGlyArgAspTyrTyrAspSerGlyGlyTyrPheThrValAla                               100105110                                                                      PheAspIleTrpGly                                                                115                                                                            (2) INFORMATION FOR SEQ ID NO:25:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 111 amino acids                                                    (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:25:                                       AspValLeuMetThrGlnThrProLeuSerLeuProValSerLeuGly                               151015                                                                         AspGlnAlaSerIleSerCysArgSerSerGlnIleIleIleHisSer                               202530                                                                         AspGlyAsnThrTyrLeuGluTrpPheLeuGlnLysProGlyGlnSer                               354045                                                                         ProLysLeuLeuIleTyrLysValSerAsnArgPheSerGlyValPro                               505560                                                                         AspArgPheSerGlySerGlySerGlyThrAspPheThrLeuMetIle                               65707580                                                                       SerArgValGluAlaGluAspLeuGlyValTyrTyrCysPheGlnGly                               859095                                                                         SerHisValProHisThrPheGlyGlyGlyThrLysLeuGluIle                                  100105110                                                                      (2) INFORMATION FOR SEQ ID NO:26:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 110 amino acids                                                    (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:26:                                       GlnSerValLeuThrGlnProProSerAlaSerGlyThrProGlyGln                               151015                                                                         ArgValThrIleSerCysSerGlyThrSerSerAsnIleGlySerSer                               202530                                                                         ThrValAsnTrpTyrGlnGlnLeuProGlyMetAlaProLysLeuLeu                               354045                                                                         IleTyrArgAspAlaMetArgProSerGlyValProAspArgPheSer                               505560                                                                         GlySerLysSerGlyAlaSerAlaSerLeuAlaIleGlyGlyLeuGln                               65707580                                                                       SerGluAspGluThrAspTyrTyrCysAlaAlaTrpAspValSerLeu                               859095                                                                         AsnAlaTyrValPheGlyThrGlyThrLysValThrValLeu                                     100105110                                                                      (2) INFORMATION FOR SEQ ID NO:27:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 111 amino acids                                                    (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:27:                                       GlnValLeuMetThrGlnThrProSerSerLeuProValThrLeuGly                               151015                                                                         GlnGlnAlaSerIleSerCysArgSerSerGlnIleIleIleHisSer                               202530                                                                         AspGlyAsnThrTyrLeuGluTrpPheLeuGlnLysProGlyGlnSer                               354045                                                                         ProLysLeuLeuIleTyrLysValSerAsnArgPheSerGlyValPro                               505560                                                                         AspArgPheSerGlySerGlySerGlyThrSerPheThrLeuAlaIle                               65707580                                                                       SerArgValGluAlaGluAspGluGlyValTyrTyrCysPheGlnGly                               859095                                                                         SerHisValProHisThrPheGlyGlyGlyThrLysLeuGluIle                                  100105110                                                                      (2) INFORMATION FOR SEQ ID NO:28:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 112 amino acids                                                    (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:28:                                       AspValValMetThrGlnSerProLeuSerLeuProValThrLeuGly                               151015                                                                         GlnProAlaSerIleSerCysArgSerSerGlnSerLeuValTyrSer                               202530                                                                         AspGlyAsnThrTyrLeuAsnTrpPheGlnGlnArgProGlyGlnSer                               354045                                                                         ProArgArgLeuIleTyrLysValSerAsnArgAspSerGlyValPro                               505560                                                                         AspArgPheSerGlySerGlySerGlyThrAspPheThrLeuLysIle                               65707580                                                                       SerArgValGluAlaGluAspValGlyValTyrTyrCysMetGlnGly                               859095                                                                         ThrHisTrpSerTrpThrPheGlyGlnGlyThrLysValGluIleLys                               100105110                                                                      (2) INFORMATION FOR SEQ ID NO:29:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 111 amino acids                                                    (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:29:                                       AspValLeuMetThrGlnSerProLeuSerLeuProValThrLeuGly                               151015                                                                         GlnProAlaSerIleSerCysArgSerSerGlnIleIleIleHisSer                               202530                                                                         AspGlyAsnThrTyrLeuGluTrpPheGlnGlnArgProGlyGlnSer                               354045                                                                         ProArgLeuLeuIleTyrLysValSerAsnArgPheSerGlyValPro                               505560                                                                         AspArgPheSerGlySerGlySerGlyThrAspPheThrLeuLysIle                               65707580                                                                       SerArgValGluAlaGluAspValGlyValTyrTyrCysPheGlnGly                               859095                                                                         SerHisValProHisThrPheGlyGlyGlyThrLysValGluIle                                  100105110                                                                      (2) INFORMATION FOR SEQ ID NO:30:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 112 amino acids                                                    (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:30:                                       AspIleValMetThrGlnSerProAspSerLeuAlaValSerLeuGly                               151015                                                                         GluArgAlaThrIleAsnCysLysSerSerGlnSerValLeuTyrSer                               202530                                                                         SerAsnAsnLysAsnTyrLeuAlaTrpTyrGlnGlnLysProGlyGln                               354045                                                                         ProProLysLeuLeuIleTyrTrpAlaSerThrArgGluSerGlyVal                               505560                                                                         ProAspArgPheSerGlySerGlySerGlyThrAspPheThrLeuThr                               65707580                                                                       IleSerSerLeuGlnAlaGluAspValAlaValTyrTyrCysGlnGln                               859095                                                                         TyrAspThrIleProThrPheGlyGlyGlyThrLysValGluIleLys                               100105110                                                                      (2) INFORMATION FOR SEQ ID NO:31:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 111 amino acids                                                    (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:31:                                       AspValLeuMetThrGlnThrProAspSerLeuProValSerLeuGly                               151015                                                                         AspArgAlaSerIleSerCysArgSerSerGlnIleIleIleHisSer                               202530                                                                         AspGlyAsnThrTyrLeuGluTrpPheLeuGlnLysProGlyGlnSer                               354045                                                                         ProLysLeuLeuIleTyrLysValSerAsnArgPheSerGlyValPro                               505560                                                                         AspArgPheSerGlySerGlySerGlyThrAspPheThrLeuMetIle                               65707580                                                                       SerArgValGluAlaGluAspLeuGlyValTyrTyrCysPheGlnGly                               859095                                                                         SerHisValProHisThrPheGlyGlyGlyThrLysLeuGluIle                                  100105110                                                                      (2) INFORMATION FOR SEQ ID NO:32:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 117 amino acids                                                    (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:32:                                       AspValGlnLeuValGluSerGlyGlyGlyLeuValGlnProGlyGly                               151015                                                                         SerArgLysLeuSerCysAlaAlaSerGlyPheThrPheSerSerPhe                               202530                                                                         GlyMetHisTrpValArgGlnAlaProGluLysGlyLeuGluTrpVal                               354045                                                                         AlaTyrIleSerSerGlySerPheThrIleTyrHisAlaAspThrVal                               505560                                                                         LysGlyArgPheThrIleSerArgAspAsnProLysAsnThrLeuPhe                               65707580                                                                       LeuGlnMetThrSerLeuArgSerGluAspThrAlaMetTyrTyrCys                               859095                                                                         AlaArgMetArgLysGlyTyrAlaMetAspTyrTrpGlyGlnGlyThr                               100105110                                                                      ThrValThrValSer                                                                115                                                                            (2) INFORMATION FOR SEQ ID NO:33:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 125 amino acids                                                    (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:33:                                       GluValGlnLeuValGlnSerGlyGlyGlyValValGlnProGlyArg                               151015                                                                         SerLeuArgLeuSerCysSerSerSerGlyPheIlePheSerSerTyr                               202530                                                                         AlaMetTyrTrpValArgGlnAlaProGlyLysGlyLeuGluTrpVal                               354045                                                                         AlaIleIleTrpAspAspGlySerAspGlnHisTyrAlaAspSerVal                               505560                                                                         LysGlyArgPheThrIleSerArgAsnAspSerLysAsnThrLeuPhe                               65707580                                                                       LeuGlnMetAspSerLeuArgProGluAspThrGlyValTyrPheCys                               859095                                                                         AlaArgAspGlyGlyHisGlyPheCysSerSerAlaSerCysPheGly                               100105110                                                                      ProAspTyrTrpGlyGlnGlyThrProValThrValSer                                        115120125                                                                      (2) INFORMATION FOR SEQ ID NO:34:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 117 amino acids                                                    (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:34:                                       GluValGlnLeuValGluSerGlyGlyGlyValValGlnProGlyArg                               151015                                                                         SerLeuArgLeuSerCysAlaAlaSerGlyPheIlePheSerSerPhe                               202530                                                                         GlyMetHisTrpValArgGlnAlaProGlyLysGlyLeuGluTrpVal                               354045                                                                         AlaTyrIleSerSerAspGlyPheThrIleTyrHisAlaAspSerVal                               505560                                                                         LysGlyArgPheThrIleSerArgAspAspProLysAsnThrLeuPhe                               65707580                                                                       LeuGlnMetThrSerLeuArgSerGluAspThrAlaMetTyrTyrCys                               859095                                                                         AlaArgMetArgLysGlyTyrAlaMetAspTyrTrpGlyGlnGlyThr                               100105110                                                                      ThrValThrValSer                                                                115                                                                            (2) INFORMATION FOR SEQ ID NO:35:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 120 amino acids                                                    (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:35:                                       GlnValGlnLeuValGluSerGlyGlyGlyValValGlnProGlyArg                               151015                                                                         SerLeuArgLeuSerCysAlaAlaSerGlyPheThrPheSerSerTyr                               202530                                                                         AlaMetHisTrpValArgGlnAlaProGlyLysGlyLeuGluTrpVal                               354045                                                                         AlaValIleSerTyrAspGlySerAsnLysTyrTyrAlaAspSerVal                               505560                                                                         LysGlyArgPheThrIleSerArgAspAsnSerLysAsnThrLeuTyr                               65707580                                                                       LeuGlnMetAsnSerLeuArgAlaGluAspThrAlaValTyrTyrCys                               859095                                                                         AlaArgAspArgLysAspTrpGlyTrpAlaLeuPheAspTyrTrpGly                               100105110                                                                      GlnGlyThrLeuValThrValSer                                                       115120                                                                         (2) INFORMATION FOR SEQ ID NO:36:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 117 amino acids                                                    (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:36:                                       GlnValGlnLeuValGluSerGlyGlyGlyValValGlnProGlyArg                               151015                                                                         SerLeuArgLeuSerCysAlaAlaSerGlyPheThrPheSerSerPhe                               202530                                                                         GlyMetHisTrpValArgGlnAlaProGlyLysGlyLeuGluTrpVal                               354045                                                                         AlaTyrIleSerSerGlySerPheThrIleTyrTyrAlaAspSerVal                               505560                                                                         LysGlyArgPheThrIleSerArgAspAsnSerLysAsnThrLeuTyr                               65707580                                                                       LeuGlnMetAsnSerLeuArgAlaGluAspThrAlaValTyrTyrCys                               859095                                                                         AlaArgMetArgLysGlyTyrAlaMetAspTyrTrpGlyGlnGlyThr                               100105110                                                                      LeuValThrValSer                                                                115                                                                            (2) INFORMATION FOR SEQ ID NO:37:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 98 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:37:                                       GluValGlnLeuValGluSerGlyGlyGlyLeuValGlnProGlyGly                               151015                                                                         SerLeuArgLeuSerCysAlaAlaSerGlyPheThrPheSerSerTyr                               202530                                                                         TrpMetSerTrpValArgGlnAlaProGlyLysGlyLeuGluTrpVal                               354045                                                                         AlaAsnIleLysGlnAspGlySerGluLysTyrTyrValAspSerVal                               505560                                                                         LysGlyArgPheThrIleSerArgAspAsnAlaLysAsnSerLeuTyr                               65707580                                                                       LeuGlnMetAsnSerLeuArgAlaGluAspThrAlaValTyrTyrCys                               859095                                                                         AlaArg                                                                         (2) INFORMATION FOR SEQ ID NO:38:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 117 amino acids                                                    (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:38:                                       GluValGlnLeuValGluSerGlyGlyGlyLeuValGlnProGlyGly                               151015                                                                         SerLeuArgLeuSerCysAlaAlaSerGlyPheThrPheSerSerPhe                               202530                                                                         GlyMetHisTrpValArgGlnAlaProGlyLysGlyLeuGluTrpVal                               354045                                                                         AlaTyrIleSerSerGlySerPheThrIleTyrHisAlaAspSerVal                               505560                                                                         LysGlyArgPheThrIleSerArgAspAsnAlaLysAsnThrLeuPhe                               65707580                                                                       LeuGlnMetThrSerLeuArgAlaGluAspThrAlaMetTyrTyrCys                               859095                                                                         AlaArgMetArgLysGlyTyrAlaMetAspTyrTrpGlyGlnGlyThr                               100105110                                                                      ThrValThrValSer                                                                115                                                                            (2) INFORMATION FOR SEQ ID NO:39:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 15 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:39:                                       MetGlyTrpSerCysIleIleLeuPheLeuValAlaThrAlaThr                                  151015                                                                         (2) INFORMATION FOR SEQ ID NO:40:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:40:                                       LysThrSerLeuArgProGlyLysGlySerSerAspTyrGluLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:41:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:41:                                       LysThrSerLeuArgProGlyLysGlySerSerGluTyrGluLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:42:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:42:                                       GlnThrSerLeuArgProAspLysGlySerSerAspHisGluLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:43:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:43:                                       GlnThrSerLeuArgProAspLysGlySerSerAspGlnGluLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:44:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:44:                                       GlnSerSerLeuArgProAspLysGlySerSerAspGlnGluLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:45:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:45:                                       GlnThrSerLeuArgProAspLysGlySerSerAspProGluLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:46:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:46:                                       GlnThrSerLeuArgProAspLysGlySerSerAspProGlxLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:47:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:47:                                       GlnThrSerLeuArgProAspLysGlySerSerAspProGluLysThr                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:48:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:48:                                       GlnThrSerLeuArgAlaAspLysGlySerSerAspGlnGluLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:49:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:49:                                       GlnThrSerLeuArgProAspLysGlyLysSerAspSerGluLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:50:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:50:                                       GlnThrSerLeuArgProAlaArgGlySerSerAspGlnGluLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:51:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:51:                                       GlnThrSerLeuLysProGlyArgGlySerSerAspProGluLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:52:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:52:                                       GlnThrSerLeuArgProGlyArgGlySerSerAspThrGluLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:53:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:53:                                       GlnIleSerLeuArgProGlyLysGlySerSerAspSerGluLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:54:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:54:                                       GlnThrSerLeuArgProGlyLysGlyAspSerAspGluAspLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:55:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:55:                                       GluThrAlaLeuArgProGlyLysGlyAlaSerAspAlaAspLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:56:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:56:                                       ValThrAlaLeuArgProGlyLysGlyAlaSerAspGluAspLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:57:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:57:                                       ValThrAlaLeuArgProGlyLysGlyAlaSerAspGluGluLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:58:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:58:                                       ValThrAlaLeuArgProGlyLysGlyAlaSerAsxAlaAsxLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:59:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:59:                                       ValThrAlaLeuArgProGlyLysGlyAlaSerAspGluAspAspGlu                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:60:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:60:                                       GlnThrSerLeuArgProAspLysGlySerSerAspGlnGluThrThr                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:61:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:61:                                       GlnAsnSerLeuThrProGlyLysGlySerSerSerProGluLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:62:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:62:                                       ValThrLysValArgProGlyLysGlyAspSerAspSerAspLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:63:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:63:                                       ValThrLysValArgProGlyLysGlyAspSerAspAlaGluLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:64:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:64:                                       ValThrArgValArgProGlyLysGlyAspSerAspAlaGluLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:65:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:65:                                       LeuThrLysValArgProGlyLysGlyAspSerAspSerGluLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:66:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:66:                                       ValThrLysValArgProGlyLysGlyAspSerAspSerGluGlnLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:67:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:67:                                       ValThrLysValArgProGluLysGlyAspSerAspAlaGluLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:68:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:68:                                       ValThrLysValArgProGluLysGlyAspSerAspSerGluLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:69:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:69:                                       ValThrLysValSerProGlyLysGlyAspSerAspAlaGluLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:70:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:70:                                       ValThrLysValArgSerGlyLysGlyGluSerAspAlaGluLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:71:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:71:                                       ValThrSerValLysProGlyLysGlyAspSerAspAlaGluLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:72:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:72:                                       ValSerSerValLysProGlyLysGlyAspSerAspAlaGluLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:73:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:73:                                       ValThrSerAlaLysProGlyLysGlyAspSerAspAlaGluLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:74:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:74:                                       ValSerSerAlaLysProGlyLysGlyAspSerAspAlaGluLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:75:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:75:                                       ValThrSerAlaArgProGlyLysGlyAspSerAspAlaGluLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:76:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:76:                                       ValSerProAlaLysProGlyLysGlyAspSerAspAlaGluLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:77:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:77:                                       ValThrLysAlaArgProGlyLysGlyAspSerAspValGluLysAsn                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:78:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:78:                                       ValThrLeuIleProProGlyLysGlyAspSerAspAlaGluLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:79:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:79:                                       ValThrLeuLeuGlnProGlyLysGlyAspSerAspAlaGluLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:80:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:80:                                       ValThrLeuLeuGlnProGlyLysGlyAspSerAspAlaAspLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:81:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:81:                                       ValThrLeuLeuGlnProGlyLysGlyAspSerAspAlaGluArgLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:82:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:82:                                       ValThrLeuLeuGlnAlaGlyLysGlyAspSerAspAlaGluLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:83:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:83:                                       ValThrLeuLeuGlnProGlyGluGlyAspSerAspAlaGluLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:84:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:84:                                       LeuThrLeuLeuGlnProGlyAsnGlyAspSerAspAlaGluLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:85:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:85:                                       ValThrLeuLeuGlnProGlyLysGlyAspSerAspAlaGluLysIle                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:86:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:86:                                       ValThrLeuPheGlnProGlyGlnGlyAspSerAspProGluLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:87:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:87:                                       ValThrLeuProGlnProGlyLysGlyAspSerAspAlaGluLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:88:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:88:                                       ValThrLeuProGlnProGlyLysGlyAspTrpAspAlaGluLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:89:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:89:                                       ValThrPheLeuSerProGlyGlnGlyAspSerAspAlaGluLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:90:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:90:                                       GluSerSerAlaArgProGlyLysGlyAspSerAspAlaGluLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:91:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:91:                                       ValThrLeuSerSerProGlyGlnGlyAspSerAspAlaGluLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:92:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:92:                                       ValThrThrAlaLysProGluLysGlyAspSerAspValGluLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:93:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:93:                                       ValThrThrProLysProAspLysGlyAspSerAspValGluLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:94:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:94:                                       ValThrAlaProArgProGlyLysGlyAlaSerSerAlaGluLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:95:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:95:                                       ValThrAlaProLysProGlyLysGlyThrSerSerAlaGluLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:96:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:96:                                       ValThrThrProLysProGlyLysGlyAlaSerSerAlaGluLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:97:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:97:                                       ValSerAlaProLysProGlyLysGlyAlaSerSerAlaGluLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:98:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:98:                                       ValThrAlaProArgSerGlyLysGlyAlaSerSerAlaGluLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:99:                                              (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:99:                                       ValThrAlaProLysSerGlyLysGlyAlaSerSerAlaGluLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:100:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:100:                                      ValThrAlaProLysProAspLysGlyValSerSerAlaGluLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:101:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:101:                                      ValThrAlaProLysSerGluLysGlyValSerSerAlaGluLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:102:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:102:                                      PheThrAlaProLysProGlyLysGlyAlaSerSerAlaGluLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:103:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:103:                                      LeuThrAlaProLysProGlyArgGlyValSerSerAlaGluLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:104:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:104:                                      ValThrAlaProLysSerGlyLysGlyAlaSerSerAlaGluLysArg                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:105:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:105:                                      ValSerAlaProLysProGlyLysGluGlySerSerAlaGluLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:106:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:106:                                      ValThrAlaProLysProArgLysGlyAlaSerSerAlaGluLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:107:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:107:                                      ValThrPheLeuSerProGlyGlnGlyAsnSerAspAlaGluLeuPro                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:108:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:108:                                      ValThrPheLeuSerProGlyGlnGlyAsnSerAspGluAspLeuPro                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:109:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:109:                                      ValThrLeuSerSerProGlnArgGlyAspSerAspAlaGluLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:110:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:110:                                      ValThrAlaProLysSerSerLysGlyGlySerSerAlaGluLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:111:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:111:                                      GlnThrSerProThrProGlyLysGlySerSerAspProGluLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:112:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:112:                                      GlnIleSerLeuIleProGlyLysGlySerTyrAspAspGluLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:113:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:113:                                      ValThrAlaLeuLysSerGlyLysGlyAlaSerSerAlaGluLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:114:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:114:                                      ValThrAlaLeuLysSerAspLysGlyAlaSerSerGlyGluLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:115:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:115:                                      ValThrProProSerProGlyGlnGlyAspSerAlaAlaGluLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:116:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:116:                                      ValThrProProSerProGlyGlnGlyAspSerAlaArgGluLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:117:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:117:                                      ValThrValArgLysProGlyLysGlyAspSerSerAspGluLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:118:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:118:                                      GlnThrSerValArgLeuGlyGlnGlySerSerAspProGluLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:119:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:119:                                      LysThrSerLeuArgProTrpLysGlySerSerAspSerAspLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:120:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:120:                                      GlnThrAspValThrGlnGlyGlnGlySerSerGlnProGluLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:121:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:121:                                      GlnThrAlaValSerGlnGlyGlnGlySerSerGlnSerGluLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:122:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:122:                                      LeuThrAlaProArgThrAsnArgGlySerSerAspSerGluLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:123:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:123:                                      ValThrAlaProSerSerHisArgGlySerSerAspThrGluLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:124:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:124:                                      LeuLeuSerLeuSerProLeuLysGlyAspSerAspProGluLysVal                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:125:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:125:                                      ValThrAlaProThrProAspThrGlyAlaIleLysThrGluLysLeu                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:126:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:126:                                      ValThrIleProThrProAspThrGlyAlaIleLysThrGluLysLeu                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:127:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:127:                                      AlaValSerProThrProAspThrGlyAlaIleLysThrGluLysLeu                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:128:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:128:                                      AlaValSerProThrProAspThrGlyAlaIleLysThrGluLysLeu                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:129:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:129:                                      AlaValSerProThrProAspThrGlyValIleLysThrGluLysLeu                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:130:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:130:                                      AlaValSerProThrProAspThrGlyAlaIleLysThrGluProSer                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:131:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:131:                                      TyrLeuProProThrProGlyValIleArgSerThrAlaMetLysLeu                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:132:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:132:                                      TyrLeuProProThrProGlyValIleArgSerThrAlaMetArgLeu                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:133:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:133:                                      TyrLeuProProThrProGlyLeuIleArgSerThrSerMetLysLeu                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:134:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:134:                                      TyrLeuProProThrProGlyLeuIleArgSerThrSerValLysLeu                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:135:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:135:                                      TyrLeuProProThrProGlyValIleArgSerThrAlaGluLysLeu                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:136:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:136:                                      TyrLeuProProThrProGlyValIleArgSerThrAlaGlyLysLeu                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:137:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:137:                                      TyrLeuProAlaThrProGlyValValArgSerSerAlaGlyMetLeu                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:138:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:138:                                      SerLeuProProSerProGlyLysValArgSerThrAlaGluLysLeu                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:139:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:139:                                      SerLeuProProSerProGlyLysValArgSerThrAlaAsnLysLeu                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:140:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:140:                                      SerLeuProProArgProGlyLysValArgSerSerSerGluLysLeu                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:141:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:141:                                      SerLeuProProArgProGlyLysValArgSerSerSerAspLysLeu                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:142:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:142:                                      SerLeuProProArgProGlyArgValArgSerSerSerGluLysLeu                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:143:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:143:                                      SerLeuProProArgProGlyLysValArgSerSerSerGluGlnLeu                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:144:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:144:                                      SerLeuProProArgProGlyLysValArgSerSerSerGluThrLeu                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:145:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:145:                                      SerLeuProProLysProGlyLysIleArgSerSerThrGlyLysLeu                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:146:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:146:                                      SerLeuProProLysProGlyArgIleArgSerSerThrGlyLysLeu                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:147:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:147:                                      SerLeuProProLysProGlyLysIleArgSerSerThrGlyGlnLeu                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:148:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:148:                                      SerLeuProProGluProGlyLysIleArgSerSerThrGlyArgLeu                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:149:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:149:                                      SerLeuAlaProSerProGlyLysIleArgSerThrAlaGluLysLeu                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:150:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:150:                                      SerLeuProProArgProGlyLysIleArgSerSerThrGlyAsnVal                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:151:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:151:                                      SerLeuArgProSerProGlyLysValArgSerThrAlaGluLysLeu                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:152:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:152:                                      SerLeuArgProSerProGlyLysValArgSerThrAlaAspLysLeu                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:153:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:153:                                      SerLeuArgProSerProGlyLysValArgSerThrAlaGluAsnLeu                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:154:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:154:                                      SerLeuArgProSerProGlyLysValArgSerAlaValGluLysLeu                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:155:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 15 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:155:                                      SerLeuProProArgProGlyLysArgSerSerAlaGluLysLeu                                  151015                                                                         (2) INFORMATION FOR SEQ ID NO:156:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:156:                                      SerLeuAlaProSerProGlyLysValArgSerThrValGluArgLeu                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:157:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:157:                                      SerLeuAlaProSerProAspLysIleArgSerThrProAspLysLeu                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:158:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:158:                                      SerLeuAlaLeuSerProGlyLysValArgSerThrAlaGluLysLeu                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:159:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:159:                                      SerLeuProLeuSerAlaGlyLysValArgSerThrAlaGluLysLeu                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:160:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:160:                                      SerLeuAlaProSerProGlyLysValArgSerThrAlaGluTyrLeu                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:161:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:161:                                      SerLeuProLeuThrProGlyLeuIleArgSerThrAlaGluLysLeu                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:162:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:162:                                      SerLeuProLeuThrProArgValIleArgSerThrAlaGluLysLeu                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:163:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:163:                                      PheLeuHisProThrProGlyThrAspSerSerSerThrGluLysLeu                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:164:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:164:                                      PheLeuLeuProThrProGlyThrAspSerSerSerThrGluArgLeu                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:165:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:165:                                      PheLeuHisProThrArgValThrAspSerSerSerThrGluLysLeu                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:166:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:166:                                      LeuLeuProProThrProGlyThrAsnSerSerSerAsnAspLysLeu                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:167:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:167:                                      ValLeuProLeuSerProHisArgIleArgSerGluSerGluAsnLeu                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:168:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:168:                                      SerLeuAlaProSerProAlaLysPheArgSerThrAlaGluArgAsp                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:169:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:169:                                      ValThrAlaProArgProGlyArgIleArgSerAspProGluLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:170:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:170:                                      ValThrAlaProArgProGlyArgValArgSerAspProGluLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:171:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:171:                                      ValThrGlyProArgProGlyArgIleArgSerAspProGluLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:172:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:172:                                      ValThrGlyProArgProGlyArgIleArgSerAspProAspLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:173:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:173:                                      ValThrGlyProArgProGlyArgValArgSerAspProGluLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:174:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:174:                                      ValThrGlyProArgProGlyArgIleArgSerAspProXaaLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:175:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:175:                                      ValThrAlaProArgProGlyArgIleArgSerGluSerGluArgLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:176:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:176:                                      ValThrGlyProSerArgGlyArgIleArgSerAspProGluLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:177:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:177:                                      ValThrValProArgProSerArgIleArgSerGluSerGluArgLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:178:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:178:                                      ValThrAlaProGlyProGlyArgIleArgSerGluSerGluArgLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:179:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:179:                                      GlnThrSerValArgProGlyArgValArgSerAspProGluArgLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:180:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:180:                                      GlnThrSerValArgProGlyLysValArgSerAspProGluArgLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:181:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:181:                                      GlnThrSerValArgProGlyLysValArgSerAspProGluLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:182:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:182:                                      GlnThrSerValArgProGlyLysValArgSerGluProGluLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:183:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:183:                                      GlnThrSerValArgProGlyLysValArgSerGluProAspLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:184:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:184:                                      GlnThrSerValArgProGlyLysValArgAlaGluProGluLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:185:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:185:                                      GlnThrSerValArgProGlyLysValArgSerAsxProGlxLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:186:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:186:                                      GlnThrSerValArgProGlyLysValArgSerAspProAsxLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:187:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:187:                                      GlnThrSerValArgProGlyGlnValArgSerAspProGluArgLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:188:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:188:                                      GlnThrSerValArgProGlyLysValArgSerHisProGluLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:189:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:189:                                      GlnThrSerValArgProGlyAsnValArgSerAspProAspLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:190:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:190:                                      GlnThrSerValArgProGlyLysValArgSerAspProGluLysThr                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:191:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:191:                                      GlnThrSerValArgProGlyThrValArgSerGluProGluLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:192:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:192:                                      GlnThrSerValArgProGluLysValArgSerGluProAspLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:193:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:193:                                      GlnThrSerValArgProGlyLysValArgSerGluSerAspLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:194:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:194:                                      GlnThrSerValArgProGlyGluValArgSerGluProAspLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:195:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:195:                                      GlnThrSerValArgProGlyAsxValArgSerAsxProGlxArgLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:196:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:196:                                      GlnThrSerValSerProGlyLysValArgSerAspProGluLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:197:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:197:                                      GlnThrSerValArgProGlyLysValAsnSerAspProGluLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:198:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:198:                                      GlnThrSerValArgProGlyLysValArgSerAspProAspThrLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:199:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:199:                                      GlnThrSerValArgProLysLysValArgSerAspProGlxLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:200:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:200:                                      GlnThrSerValArgProLysLysValArgPheAspProGluLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:201:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:201:                                      GlnThrSerValArgSerGlyLysValArgSerGluProGluThrLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:202:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:202:                                      ValThrAsnLeuArgProGlyLysValArgSerAspAlaGluLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:203:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:203:                                      ValThrAspLeuArgProGlyLysValArgSerAspAlaGluLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:204:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:204:                                      GlnThrSerValSerProGlyAsnIleArgSerGluSerAspLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:205:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:205:                                      LysThrSerValThrProGlyLysPheArgSerGluProGluLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:206:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:206:                                      ValThrLeuLeuProProGlyArgValArgSerAspAlaGluLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:207:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:207:                                      ValThrLeuLeuProProGlyGluValArgSerAspAlaGluLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:208:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:208:                                      ValThrLeuProProProGlyGlxValArgSerAspAlaGluArgLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:209:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:209:                                      ValThrLeuProProProGlyGlxValArgSerAsxAlaGlxAsnLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:210:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:210:                                      ValThrLeuProProProGlnGlnValArgSerAspAlaGluLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:211:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:211:                                      ValThrLeuProProProGlyGlnValThrSerAspAlaGluLysLys                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:212:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:212:                                      ValThrLeuProProAlaGlyGlnValArgSerAspAlaGluLysArg                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:213:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 16 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:213:                                      AlaLeuSerProSerSerGlyGlnSerSerSerAlaSerGluArgLeu                               151015                                                                         (2) INFORMATION FOR SEQ ID NO:214:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:214:                                      GluLysValGlyGlyLeuGlnProGlyArgGlyThrProGlyLysAla                               151015                                                                         SerArgGlyAspSerGlnArgProGluSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:215:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:215:                                      GluLysValGlyGlyLeuGlnProGlyArgGlyThrProGlyLysVal                               151015                                                                         SerArgGlyAspSerGlnArgProGluSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:216:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:216:                                      GluLysValGlyGlyLeuGlnProGlyThrGlyAlaProGlyLysAla                               151015                                                                         SerArgGlyAspSerGlnArgProGluSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:217:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:217:                                      GluLysValGlyGlyLeuGlnProGlyArgGlyThrProGlyLysAla                               151015                                                                         SerLysGlyAsnSerGlnArgAlaGluSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:218:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:218:                                      GluLysMetGlyGlyLeuGlnProGlyArgGlyThrProGlyLysAla                               151015                                                                         SerLysGlyAsnSerGlnArgAlaGluSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:219:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:219:                                      GluLysValGlyGlyLeuGlnProGlyArgGlyThrProGlyLysAla                               151015                                                                         SerLysGlyThrSerGlnArgAlaGluSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:220:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:220:                                      GluLysValGlyGlyLeuGlnProGlyArgGlyThrProGlyLysAla                               151015                                                                         SerLysGlyThrSerGlnArgAlaGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:221:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:221:                                      GluLysValGlyGlyLeuLysProGlyArgGlyThrProGlyLysAla                               151015                                                                         SerLysGlyThrSerGlnArgAlaGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:222:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:222:                                      GluAsnValGlyGlyLeuGlnProGlyArgGlyThrProGlyLysAla                               151015                                                                         SerLysGlyThrSerGlnArgAlaGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:223:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:223:                                      GluLysValGlyGlyLeuGlnSerGlyArgGlyThrProGlyLysAla                               151015                                                                         SerLysGlyThrSerGlnArgAlaGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:224:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:224:                                      GluLysValGlyGlyLeuGlnSerGlyArgGlyThrProGlyLysAla                               151015                                                                         SerLysGlyThrSerGlnArgAlaGluSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:225:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:225:                                      GluLysValGlyGlyLeuGlnProGlyArgGlyThrProGlyLysAla                               151015                                                                         SerLysGlyIleSerGlnArgAlaGluArg                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:226:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:226:                                      GluLysValGlyGlyLeuGlnProGlyArgGlyThrProGlyLysSer                               151015                                                                         AlaLysGlyAsxSerGlxArgAlaGlnSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:227:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:227:                                      GluLysValGlyGlyLeuGlnProGlySerGlyThrProGlyLysAla                               151015                                                                         SerLysGlyAsnSerGlnArgAlaGluSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:228:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:228:                                      GluLysValGlyGlyLeuGlnProGlySerGlyThrProGlyLysAla                               151015                                                                         SerLysGlySerSerGlnArgAlaGluSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:229:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:229:                                      GluLysValGlyGlyLeuGlnProGlyArgGlyThrProArgLysAla                               151015                                                                         SerLysGlyAsnSerGlnArgAlaGluSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:230:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:230:                                      GluLysMetGlyAsnLeuGlnProGlySerGlyThrProGlyLysAla                               151015                                                                         SerLysGlyAsnSerGlnArgProAspSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:231:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:231:                                      GluLysValGlyGlyLeuLysProGlyLysGlyThrProGluLysAsp                               151015                                                                         SerLysGlyAsnAlaArgArgSerGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:232:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:232:                                      GluLysValGlyGlyLeuLysProGlyLysGlyAlaProGluLysAsp                               151015                                                                         SerLysGlyAsnAlaArgArgSerGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:233:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:233:                                      GluLysValGlyGlyLeuLysProGlyLysGlyThrProGluArgAsp                               151015                                                                         SerLysGlyAsnAlaArgArgSerGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:234:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:234:                                      AspLysValGlyGlyLeuLysProGlyLysGlyThrProGluLysAsp                               151015                                                                         SerLysGlyAsnAlaLysArgSerGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:235:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:235:                                      AspLysValGlyGlyLeuLysProGlyLysGlyThrProGluLysAsp                               151015                                                                         SerLysGlyAsnAlaLysLysSerGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:236:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:236:                                      AspLysValGlyGlyLeuLysProGlyLysGlyThrProAspLysAsp                               151015                                                                         AsnLysGlyAsnAlaLysLysSerGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:237:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:237:                                      GluLysValGlyGlyLeuThrProGlyLysGlyThrProGluLysAsp                               151015                                                                         SerLysGlyAsnGlyArgArgSerGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:238:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:238:                                      GluMetValGlyGlyLeuLysProGlyLysGlyThrProGluLysAsp                               151015                                                                         SerLysGlyAsnAspArgArgSerGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:239:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:239:                                      GluMetValGlyGlyLeuLysProGlyLysGlyThrProGluLysAsp                               151015                                                                         SerLysGlyAsnAspLysArgSerGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:240:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:240:                                      GluMetValGlyGlyLeuLysProGlyLysGlyThrProGluLysAsp                               151015                                                                         SerLysGlyAsnAlaLysArgSerGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:241:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:241:                                      GluGlnValGlyGlyLeuLysProGlyLysGlyThrProGluLysAsp                               151015                                                                         SerLysGlyAsnAlaLysLysSerGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:242:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:242:                                      GluGlnValGlyGlyLeuLysProGlyLysGlyThrProGluLysAsp                               151015                                                                         ThrLysGlyAsnAlaLysLysSerGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:243:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:243:                                      GluGlnValGlyGlyLeuLysProGlyLysGlyAlaProGluLysAsp                               151015                                                                         ThrLysGlyAsnAlaLysLysSerGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:244:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:244:                                      GluLysValGlyGlyLeuGlnProGlyLysGlyThrProGluLysAsp                               151015                                                                         SerLysGlyAsnAlaLysLysSerGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:245:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:245:                                      GluLysValGlyGlyLeuGlnProGlyLysGlyThrProGluLysAsp                               151015                                                                         ThrLysGlyAsnAlaLysLysSerGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:246:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:246:                                      GluLysValGlyGlyLeuGlnProGlyArgGlyThrProGluLysAsp                               151015                                                                         ThrLysGlyAsnAlaLysLysSerGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:247:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:247:                                      GluLysValGlyGlyLeuGlnProGlyLysGlySerProGluLysAsp                               151015                                                                         SerLysGlyAsnAlaLysLysSerGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:248:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:248:                                      AspLysMetGlyGlyLeuLysProGlyLysGlyThrProGluLysAsp                               151015                                                                         SerLysGlyAsnAlaLysGlnSerGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:249:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:249:                                      GluGlnValGlyGlyLeuGlnProGlyLysGlyThrProAspLysAsp                               151015                                                                         SerLysGlyAsnAlaLysLysSerGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:250:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:250:                                      GluLysValGlyGlyLeuGlnProGlyLysGlyThrProGluLysAsp                               151015                                                                         SerLysGlyAsnAlaGluLysSerGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:251:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:251:                                      GluGlnValGlyAspLeuLysProGlyLysGlyThrProGluLysAsp                               151015                                                                         ThrLysGlyAsnAlaArgArgSerGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:252:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:252:                                      GluAsnValGlyAspLeuLysProGlyLysGlyAlaProGluLysAsp                               151015                                                                         SerLysGlyAsnAlaArgArgSerGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:253:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:253:                                      GluGlnValGlyGlyLeuGlnProGlyLysGlyThrSerAspLysAsp                               151015                                                                         SerLysGlyAsnAlaLysLysSerGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:254:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:254:                                      GluGlnValGlyGlyLeuGlnProGlyLysGlyThrProGluLysAsp                               151015                                                                         SerLysGlyAsnAlaLysLysSerGlyThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:255:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:255:                                      AspGlnValGlyGlyLeuGlnProGlyLysGlyThrProGluLysAsp                               151015                                                                         ThrLysGlyAsnProLysArgSerGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:256:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:256:                                      AspGlnValGlyGlyLeuGlnProGlyGlnGlyThrProGluLysAsn                               151015                                                                         ThrLysGlyAsnProLysArgSerAspThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:257:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:257:                                      GluLysValGlyGlyLeuGlnProGlyLysGlyThrSerGluLysAsp                               151015                                                                         IleLysGlyAsnAlaLysLysSerGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:258:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:258:                                      AspLysValGlyGlyLeuLysProGlyLysArgThrProGluLysAsp                               151015                                                                         AsnLysGlyAsnAlaLysLysSerGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:259:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:259:                                      AspLysValGlyGlyLeuLysLeuGlyLysGlyThrProGluLysAsp                               151015                                                                         ThrLysGlyAsnAlaLysLysSerGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:260:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:260:                                      GluLysValGlyGlyLeuGlnProGlyLysGlyThrProGluLysAsp                               151015                                                                         SerLysGlyAsnAlaAsnThrSerGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:261:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:261:                                      GluHisValGlyGlyLeuLysProGlyLysGlyThrProGluLysAsp                               151015                                                                         SerLysGlyAsnAlaGlyArgSerGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:262:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:262:                                      GluGlnValGlyGlyLeuGlnProGlyAsnGlyThrProGluLysAsp                               151015                                                                         ThrThrGlyAsnAlaLysArgSerGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:263:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:263:                                      GluLysGluGlyGlyLeuGlnProGlyLysGlyThrProGluLysGlu                               151015                                                                         SerLysGlyAspSerLysArgAlaGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:264:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:264:                                      GluLysGluGlyGlyLeuGlnProGlyLysGlyThrProGluLysGlu                               151015                                                                         SerLysGlyAspSerLysArgProGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:265:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:265:                                      GluLysGluGlyGlyLeuGlnProGlyLysGlySerProGluLysGlu                               151015                                                                         SerLysGlyAspSerLysArgAlaGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:266:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:266:                                      GluLysAspGlyGlyLeuGlnProGlyLysGlyThrProGluLysAsp                               151015                                                                         SerLysGlyAspSerLysArgValGluMet                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:267:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:267:                                      GluGlnValGlyGlyLeuLysProGlyArgGlyThrProGluLysAsp                               151015                                                                         ThrThrGlyAspAlaGlnArgSerGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:268:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:268:                                      GluGlnValGlyGlyLeuLysProGlyArgGlyThrProGluLysAsp                               151015                                                                         ThrThrGlyAsnAlaLysGlySerGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:269:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:269:                                      GluLysValGlyGlySerLysProGlyLysGlyThrProGluLysAsp                               151015                                                                         SerLysGlyAsnAlaLysThrSerGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:270:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:270:                                      SerAspGlnGlyGlyLeuLysProGlyLysGlyThrProGluLysAsp                               151015                                                                         ThrLysGlyAsnAlaArgArgSerGluSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:271:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:271:                                      GluLysIleGlyGlyLeuGlnProGlyLysGlyAspProGlyLysPro                               151015                                                                         SerLysAspAsnAlaLysArgSerGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:272:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:272:                                      GluLysLeuGlyGlyLeuGlnProGlyLysGlyAspProGlyLysPro                               151015                                                                         SerLysAspAsnAlaLysArgSerGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:273:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:273:                                      GluLysLeuGlyGlyLeuGlnProGlyLysGlyAspProGlyLysPro                               151015                                                                         PheLysAspAsnAlaLysArgSerGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:274:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:274:                                      GluLysLeuGlyGlyLeuGlnProGlyLysGlyAspProGlyLysLeu                               151015                                                                         MetLysGluAsnAlaLysArgSerGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:275:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:275:                                      GluAsnLeuGlyGlyLeuGlnProGlyLysGlyAspProGlyLysLeu                               151015                                                                         LysXaaGluAsnAlaLysArgProGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:276:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:276:                                      GluLysLeuGlyGlyLeuGlnProGlyAsnGlyAspLeuGlyLysPro                               151015                                                                         SerLysAspAsnAlaLysArgSerGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:277:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:277:                                      GluLysLeuGlyProLeuGlnLeuGlyLysGlyAspProGlyLysPro                               151015                                                                         SerLysAspAspAlaLysArgSerGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:278:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:278:                                      GluGlnLeuGlyGlyLeuGlnProGlyGlyGlyThrProGlyLysPro                               151015                                                                         SerLysAspAsnAspLysArgSerGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:279:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:279:                                      GluGlnLeuGlyGlyLeuGlnProGlyGlyGlyThrProGlyLysAla                               151015                                                                         SerLysAspAsnAspLysArgSerGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:280:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:280:                                      GluGlnValGlyGlyLeuLysAlaArgLysGlyThrProGluLysAsp                               151015                                                                         ThrThrGlyAsnAlaLysArgSerGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:281:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:281:                                      GluMetValGlyValLeuGluProGlyLysGlyThrProGluLysArg                               151015                                                                         GlnGluGlyAsnAlaLysArgSerGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:282:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:282:                                      GluGlnValGlyGlyLeuGlnProLysLysGlySerProGlyLysAsp                               151015                                                                         SerLysAspAspSerGlnLysThrGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:283:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:283:                                      GluGlnValGlyGlyLeuGlnProLysLysGlySerProGlyLysAsp                               151015                                                                         SerLysAspAspSerGlnLysThrGluArg                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:284:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:284:                                      GlnGlnValProGluLeuLysProGlyArgGlyThrProGlyLysGlu                               151015                                                                         AspLysGlyThrSerAlaArgAsnAspThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:285:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:285:                                      GlnGlnValProGluLeuLysProGlyLysGlyThrProGlyLysAsp                               151015                                                                         AspLysGlyThrSerAlaLysAsnGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:286:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:286:                                      GlnGlnValProGluLeuLysProGlyLysGlyThrProGlyLysAsp                               151015                                                                         AspLysGlyThrSerAlaLysAsnGluMet                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:287:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:287:                                      GlnGlnLysProGluLeuLysProGlyLysGlySerProGlyGlnGlu                               151015                                                                         LysLysGlyThrSerSerThrSerGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:288:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:288:                                      GluGlnGlnProGluLeuLysProGlyLysGlyThrProGlyGlnGlu                               151015                                                                         LysLysGlyLysSerSerThrSerGluSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:289:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:289:                                      GluGlnGlnProGluLeuArgProGlyLysGlyThrProGlyGlnGlu                               151015                                                                         LysLysGlyLysSerSerThrSerGluSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:290:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:290:                                      GluGlnGlnProGluLeuLysProGlyLysGlyThrProGlyGlnGlu                               151015                                                                         LysLysGlyLysSerSerAlaSerGluSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:291:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:291:                                      GluGlnGlnProGluLeuLysProGlyLysGlyThrProGlyLysGln                               151015                                                                         LysLysGlyLysSerSerThrSerGluSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:292:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:292:                                      GluGlnGlnProGluLeuLysProGlyLysGlyThrHisGlyLysGln                               151015                                                                         LysLysGlyLysSerSerThrSerGluSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:293:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:293:                                      GluGlnGlnProGluLeuLysProGlyLysGlySerHisGlyLysGln                               151015                                                                         LysLysGlyLysSerSerThrSerGluSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:294:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:294:                                      GluGlnGlnProGluLeuLysProGlyLysGlySerHisGlyLysGln                               151015                                                                         LysLysGlyLysSerSerAlaSerGluSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:295:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:295:                                      GluGlnGlnProGluLeuLysProGlyLysGlyThrHisGlyLysGln                               151015                                                                         LysLysGlyLysSerSerThrPheGluSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:296:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:296:                                      GluGlnGlnProGluLeuLysProGlyLysGlyThrHisGlyLysGln                               151015                                                                         LysGlnGlyLysSerSerThrPheGluSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:297:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:297:                                      GluGlnGlnProGluLeuLysProGlyLysGlyThrHisGlyLysGlu                               151015                                                                         LysLysAspLysSerSerThrSerGluSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:298:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:298:                                      GluGlnGlnAlaGluLeuLysProGlyLysGlySerHisGlyLysGln                               151015                                                                         LysLysGlyLysSerSerThrSerGluSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:299:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:299:                                      GluGlnGlnProGluLeuLysProGlyLysGlyThrHisGlyLysGln                               151015                                                                         LysLysSerAsnSerSerThrSerGluSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:300:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:300:                                      GlnGlnGlnAlaGluLeuArgProGlyLysGlyAlaProGlyGlnGlu                               151015                                                                         LysLysGlyLysSerSerThrSerGluSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:301:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:301:                                      GlnGlnGlnAlaGluLeuArgProGlyLysGlyAlaProGlyGlnGlu                               151015                                                                         LysLysGlyLysSerSerThrSerAspSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:302:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:302:                                      GlnGlnGlnAlaGluLeuArgProGlyLysGlyValProGlyGlnGlu                               151015                                                                         LysLysGlyLysSerSerThrSerAspSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:303:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:303:                                      GlnGlnGlnProGluLeuLysProGlyLysGlyAlaProGlyLysGly                               151015                                                                         LysLysGlyLysSerSerThrSerGluSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:304:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:304:                                      GlnGlnGlnProGluLeuLysProGlyLysGlyAlaProGlyLysGly                               151015                                                                         LysLysAspLysSerSerThrSerGluSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:305:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:305:                                      GluGlnGlnProGluAlaLysProGlyLysGlyThrHisGlyLysGln                               151015                                                                         LysLysGlyLysSerSerThrSerAspSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:306:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:306:                                      GlnGlnGlnAlaGluLeuLysProGlyLysGlyThrHisGlyLysGlu                               151015                                                                         LysLysAspLysSerSerThrSerAspSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:307:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:307:                                      GlnGlnGlnAlaGluLeuArgProGlyLysGlyAlaProGlyGlnGly                               151015                                                                         LysLysGlyLysSerSerThrSerGluSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:308:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:308:                                      GlnGlnGlnAlaGluLeuLysProGlyArgGlyThrProGlyGlnGlu                               151015                                                                         LysLysGlyLysSerSerThrSerGluSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:309:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:309:                                      GluGlnGlnAlaGluLeuArgAlaGlyLysGlyThrProGlyGlnGlu                               151015                                                                         LysLysGlyLysSerSerThrSerGluSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:310:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:310:                                      GluGlnGlnAlaGluLeuArgProGlyLysGlyThrProGlyGlnGlu                               151015                                                                         LysLysGlyThrSerSerThrSerGluSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:311:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:311:                                      GlnGlnGlnAlaGluLeuArgProGlyLysGlyThrProGlyHisGlu                               151015                                                                         LysLysGlyThrSerSerThrSerGluSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:312:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:312:                                      GlnGlnGlnAlaGluLeuLysProGlyLysGlyThrProGlyHisGlu                               151015                                                                         LysLysGlyThrSerSerThrSerGluSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:313:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:313:                                      GlnGlnGlnAlaGluLeuArgProGlyLysGlyThrProGlyHisGlu                               151015                                                                         AsnLysGlyThrSerSerThrSerGluSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:314:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:314:                                      GlnGlnGlnAlaGluValArgProGlyLysGlyThrProGlyHisGlu                               151015                                                                         LysLysGlyThrSerSerThrSerGluSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:315:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:315:                                      GlnGlnGlnAlaGluLeuLysProGlyLysGlyThrProGlyHisGlu                               151015                                                                         AsnLysGlyThrSerSerThrSerGluSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:316:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:316:                                      GlnGlnGlnAlaGluLeuArgProGlyLysGlyThrProGlyGlnGln                               151015                                                                         LysLysGlyLysSerSerAlaSerGluSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:317:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:317:                                      HisGlnGlnAlaGluLeuLysProGlyLysGlyThrProGlyGlnGln                               151015                                                                         LysLysGlyLysSerSerThrSerGluSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:318:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:318:                                      GluGlnGlnValGluLeuArgAlaGlyLysGlyThrProGlyGlnGlu                               151015                                                                         LysLysGlyLysSerSerThrSerGluSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:319:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:319:                                      GluGlnGlnAlaGluLeuArgProGlyLysGlyThrProGlyGlnGlu                               151015                                                                         LysGlnGlyThrSerSerThrSerGluSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:320:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:320:                                      GluGlnGlnAlaGluLeuArgProGlyLysGlyThrProGlyHisAsp                               151015                                                                         AsnLysGlyThrSerSerThrSerGluSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:321:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:321:                                      GlnGlnGlnAlaGluValArgProGlyLysGlyThrProGlyHisGlu                               151015                                                                         LysLysGlyArgSerSerThrSerGluSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:322:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:322:                                      GlnGlnGlnAlaGluLeuArgProGlyLysGlyThrProGlyGlnGln                               151015                                                                         LysLysAspLysSerSerThrSerGluSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:323:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:323:                                      GlnGlnGlnAlaGluLeuLysProGlyLysGlyThrProGlyGlnGln                               151015                                                                         LysLysAspLysSerSerThrSerGluSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:324:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:324:                                      GlnGlnGlnAlaGluLeuLysProGlyLysGlyThrProGlyGlnGln                               151015                                                                         LysLysAspLysSerSerThrSerAspSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:325:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:325:                                      GlnGlnGlnAlaGluLeuLysProGlyLysGlySerProGlyGlnGln                               151015                                                                         LysLysAspLysSerSerThrSerGluSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:326:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:326:                                      GlnHisGlnAlaGluLeuLysProGlyLysGlyThrProGlyGlnGln                               151015                                                                         LysLysAsnLysSerSerThrSerGluSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:327:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:327:                                      GlnGlnGlnAlaGluLeuLysProGlyLysGlyThrProGlyGlnGln                               151015                                                                         AsnLysAspLysSerSerThrSerGluSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:328:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:328:                                      GluGlnGlnAlaGluLeuArgAlaGlyLysGlyIleProGlyGlnGlu                               151015                                                                         LysLysGlyLysSerSerThrSerGluSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:329:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:329:                                      GlnGlnGlnAlaGluLeuLysProGlyLysGlyThrProGlyGlnGlu                               151015                                                                         LysLysSerLysSerSerThrSerGluSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:330:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:330:                                      GlnGlnGlnSerGluLeuLysProGlyLysGlyThrProGlyGlnGlu                               151015                                                                         LysLysSerLysSerSerThrSerGluSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:331:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:331:                                      GlnGlnGlnThrGluLeuLysProGlyLysGlyThrProGlyGlnGlu                               151015                                                                         LysLysSerLysSerSerThrSerGluSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:332:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:332:                                      GluGlnGlnAlaGluLeuArgThrGlyLysGlyThrProGlyGlnGlu                               151015                                                                         ArgLysGlyLysSerSerThrSerGluSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:333:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:333:                                      GlnGlnGlnAlaGluLeuLysProGlyLysGlyThrProGlyGlnGln                               151015                                                                         LysLysAspLysSerSerThrPheGluSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:334:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:334:                                      GluGlnGlnAlaGluLeuArgProGlyThrGlyAlaProGlyGlnGlu                               151015                                                                         LysLysGlyLysSerSerThrSerGluSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:335:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:335:                                      GlnGlnGlnProGluValArgProGlyLysGlyThrHisAlaLysGln                               151015                                                                         LysLysGlyLysSerSerThrSerGluSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:336:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:336:                                      GlnGlnGlnProGluValArgProGlyLysAspThrHisAlaLysGln                               151015                                                                         LysLysGlyLysSerSerThrSerGluSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:337:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:337:                                      GlnGlnGlnAlaGluLeuLysProGlyLysGlyThrProGluGlnGlu                               151015                                                                         LysLysGlyLysSerSerThrSerGluSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:338:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:338:                                      GluGlnGlnThrGluLeuArgAlaGlyLysGlyThrProGlyGlnGlu                               151015                                                                         LysLysGlyArgSerSerThrSerGluAla                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:339:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:339:                                      GlnGlnGlnAlaGluLeuLysProGlyLysGlyThrProGlyArgGlu                               151015                                                                         LysLysSerLysProSerThrSerGluSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:340:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:340:                                      GlnGlnGlnSerGluLeuLysProGlyLysGlyThrProGlyArgGlu                               151015                                                                         LysLysSerLysProSerThrSerGluSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:341:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:341:                                      GlnGlnArgAlaGluLeuLysProGlyLysAspThrProGlyArgGlu                               151015                                                                         LysLysAsnLysProSerThrSerGluSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:342:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:342:                                      GlnGlnGlnAlaGluLeuLysProGlyLysGlyThrProGlyArgGlu                               151015                                                                         LysLysSerThrSerSerThrSerGluSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:343:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:343:                                      GlnGlnGlnAlaGluLeuLysProGlyLysGlyThrProGlyGlnGlu                               151015                                                                         LysLysSerThrSerSerThrSerAspSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:344:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:344:                                      GlnGlnGlnAlaGluLeuArgProGlyLysGlyThrProIleGlnGln                               151015                                                                         LysLysAspLysSerSerThrSerGluSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:345:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:345:                                      GlnGlnGlnAlaGluPheLysProGlyLysGlyThrProGlyArgGlu                               151015                                                                         HisArgSerLysProSerThrSerGluSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:346:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:346:                                      GlnGlnGlnAlaGluLeuArgProGlyLysGlyAlaLeuGlyGlnGlu                               151015                                                                         LysLysGlyLysSerSerThrSerAspSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:347:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:347:                                      GlnGlnGlnProGluValLysProGlyLysGlyAlaProGlyLysGly                               151015                                                                         AsnThrAspLysSerSerThrSerGluSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:348:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:348:                                      GluGlnGlnAlaGluValArgAlaGlyLysGlySerProGlyGlnGlu                               151015                                                                         LysLysGlyLysSerSerThrSerGluSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:349:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:349:                                      GlnGlnLeuAlaGluLeuLysProGlyLysGlyThrProGlyHisGlu                               151015                                                                         LysLysGlyIleSerSerThrSerGluSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:350:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:350:                                      GlnGlnGlnAlaGluLeuLysProGlyLysGlyLysProGluGlnGlu                               151015                                                                         LysLysGlyThrSerSerThrSerGluSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:351:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:351:                                      GlnGlnGlnProGluLeuLysProGlyLysGlyArgAsnGlyLysGlu                               151015                                                                         AsnLysGlyLysSerSerThrSerGluSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:352:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:352:                                      GlnGlnGlnThrGluLeuArgProGlyArgGlyThrThrGlyGlnGlu                               151015                                                                         ArgLysGlyLysSerSerThrSerGluSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:353:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:353:                                      GlnHisGlnAlaGluLeuLysProGlyLysGlyThrProGlyHisGlu                               151015                                                                         AsnLysValThrSerSerThrSerGluSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:354:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:354:                                      GluGlnGlnAlaGluLeuArgAlaGlyLysGlyThrProGlyGlnGlu                               151015                                                                         GlnLysAlaLysSerSerThrSerGluSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:355:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:355:                                      GlnGlnGlnAlaGluLeuLysProGlyLysGlyThrProGlyGlnGln                               151015                                                                         LysThrGlyThrSerSerThrThrGluSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:356:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:356:                                      GlnGlnGlnAlaGluLeuLysProGlyLysGlyAsnProGlyGlnGlu                               151015                                                                         LysLysSerThrSerSerAlaSerGluSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:357:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:357:                                      GluGlnGlnThrValLeuArgProGlyLysGlyThrProGlyGlnGln                               151015                                                                         LysLysGlyThrSerAlaThrAsnGluSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:358:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:358:                                      GlnGlnLeuThrGluLeuLysProGlyAsnGlyThrProGlyGlnGlu                               151015                                                                         LysLysSerLysSerSerThrSerGluSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:359:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:359:                                      GlnGlnGlnSerValLeuArgProGlyLysGlyThrProGlyGlnGlu                               151015                                                                         LysLysGlyThrSerSerThrSerLysSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:360:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:360:                                      LeuGlnGlnProValLeuLysProGlyLysGlySerHisGlyLysGln                               151015                                                                         LysLysAspLysSerSerThrSerGluSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:361:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:361:                                      GluGlnGlnProGluThrLysProGlyLysGlyThrLeuGlyLysGln                               151015                                                                         LysLysSerLysSerSerThrSerGluSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:362:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:362:                                      GlnGlnGlnAlaGluLeuLysProGlyGlnGlyThrProGlyGlnGlu                               151015                                                                         LysLysAsnLysSerSerThrProGluPhe                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:363:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:363:                                      GluGlnGlnAlaGluLeuArgProGlyLysGlyAsnProGluGlnPro                               151015                                                                         LysGlnGlyThrSerSerThrSerGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:364:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:364:                                      GluGlnGlnAlaGluLeuArgProGlyLysGlyAsnProGluGlnPro                               151015                                                                         LysGlnGlyThrSerThrThrSerGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:365:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:365:                                      GluGlnGlnAlaGluLeuLysProGlyLysGlyAsnProGluGlnPro                               151015                                                                         LysGlnGlyThrSerSerThrSerGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:366:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:366:                                      GluGlnGlnAlaGluLeuLysProGlyLysGlyAsnProGluGlnPro                               151015                                                                         LysGlnAspThrSerSerThrSerGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:367:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:367:                                      GluGlnGlnAlaGluLeuLysProGlyLysGlyAsnProGluGlnPro                               151015                                                                         LysGlnGlyThrSerSerThrSerGlyThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:368:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:368:                                      GluGlnGlnAlaGluValLysProGlyLysGlyAsnProGluGlnPro                               151015                                                                         LysGlnGlyThrSerSerThrSerGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:369:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:369:                                      GluGlnGlnAlaGluLeuArgProGlyLysGlyAsnProGluGlnPro                               151015                                                                         LysGlnValThrSerSerThrSerGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:370:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:370:                                      GluGlnGlnAlaGluLeuArgProGlyLysGlyAsnProGluGlnPro                               151015                                                                         LysGlnIleThrSerSerThrSerGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:371:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:371:                                      GluGlnGlnAlaGluLeuArgProGlyArgGlyAsnProGluGlnPro                               151015                                                                         LysGlnValThrSerSerThrSerGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:372:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:372:                                      GluGlnGlnAlaGluLeuArgProGlyArgGlyAsnProGluGlnPro                               151015                                                                         LysHisValThrSerSerThrSerGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:373:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:373:                                      GluGlnGlnAlaGluLeuArgProGlyLysGlyAsnThrGluGlnPro                               151015                                                                         LysGlnValThrSerSerThrSerGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:374:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:374:                                      GluGlnGlnAlaGluLeuLysProGlyLysGlyAsnThrGluGlnPro                               151015                                                                         LysLeuIleThrSerSerThrSerGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:375:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:375:                                      ThrGlyGlnAlaGluLeuArgProGlyLysGlyAlaProGluGlnGly                               151015                                                                         LysLysGlyLysSerSerThrSerAspArg                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:376:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:376:                                      GlnTyrGlnAlaGluLeuArgProGlyLysGlyThrProArgGlnGln                               151015                                                                         LysLysGlyLysSerSerThrSerGluSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:377:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:377:                                      GlnGlnGlnAlaValLeuArgHisGlyLysGlyThrHisGlyGlnGlu                               151015                                                                         LysLysGlyLysSerSerThrSerGluSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:378:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:378:                                      GlnGlnGlnThrLysLeuGlyProGlyArgGlyThrProGlyGlnGly                               151015                                                                         ArgLysGlyLysSerSerThrSerGlySer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:379:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:379:                                      GluGlnGlnAlaGluLeuArgAlaGlyLysGlyThrProGlyGlnGlu                               151015                                                                         LysLysGlyLysSerSerValTyrPheAla                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:380:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:380:                                      GluGlnGlnAlaGluLeuLysAlaGlyLysGlyThrProGlyGlnGln                               151015                                                                         LysGlnGlyGluSerThrArgSerGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:381:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:381:                                      GlnGlnLysAlaGluLeuAlaAlaSerLysGlyThrProGlyGlnGlu                               151015                                                                         LysLysGlyArgSerSerThrSerGluSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:382:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:382:                                      GlnGlnGlnThrGluLeuArgProGlyLysGlyThrProGlyGlnGlu                               151015                                                                         LysArgGlyLysSerSerAsnLeuArgLeu                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:383:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:383:                                      GluLysValGlyGlyLeuGlnGlySerSerPheAspProGlyLysAla                               151015                                                                         SerLysGlyThrSerGlnArgAlaGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:384:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:384:                                      GluGlnGlnAlaAspLeuLysLeuGlyLysGlyAsnProGluGlnPro                               151015                                                                         LysLeuAlaThrProSerThrSerGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:385:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:385:                                      GluGlnValGlyGlyLeuLysProGlyLysGlyThrProAspLysSer                               151015                                                                         AspValLysAspAsnAlaLysSerGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:386:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:386:                                      AspGlnGlnProAspLeuLysProSerSerGlySerProGlyHisPro                               151015                                                                         SerLysSerThrSerLysThrThrGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:387:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:387:                                      AspGlnGlnProAspLeuLysProSerSerGlySerProGlyAsnPro                               151015                                                                         SerLysSerThrSerLysThrThrGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:388:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:388:                                      AspGlnGlnProAspLeuLysProSerSerGlySerProGlyAsnPro                               151015                                                                         SerLysSerThrSerLysThrAlaGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:389:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:389:                                      AspGlnGlnProGlyLeuLysProSerSerGlySerProGlyAsnPro                               151015                                                                         SerLysSerThrSerLysThrThrGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:390:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:390:                                      AspGlnGlnProGlyLeuLysProSerSerGlySerProGlyAsnPro                               151015                                                                         SerLysAsnThrSerLysThrThrGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:391:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:391:                                      AspGlnGlnProGlyLeuLysProSerSerGlySerProGlyAspPro                               151015                                                                         SerLysThrThrSerLysThrThrGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:392:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:392:                                      AspGlnGlnProGlyLeuLysProSerSerGlySerProGlyAsnPro                               151015                                                                         SerLysThrThrSerLysThrThrGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:393:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:393:                                      AspHisGlnProGlyLeuLysProSerSerGlySerProGlyAsnPro                               151015                                                                         SerLysAsnThrSerLysThrThrGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:394:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:394:                                      AspGlnGlnProGlyLeuLysProSerSerGlySerProGlyAsnPro                               151015                                                                         SerArgSerThrSerLysThrThrGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:395:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:395:                                      AspGlnGlnProGlyLeuLysProSerAlaGlySerProGlyAsnPro                               151015                                                                         SerLysSerThrSerLysThrAlaGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:396:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:396:                                      GluGlnGlnProGlyLeuLysProSerSerGlySerProGlyAsnPro                               151015                                                                         SerLysSerThrSerLysThrSerGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:397:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:397:                                      AspGlnGlnProGlyLeuLysProSerSerGlySerProGlyAsnPro                               151015                                                                         SerLysAsnThrSerLysThrIleGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:398:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:398:                                      AspGlnGlnProGlyLeuLysProSerSerGlySerProGlyAspPro                               151015                                                                         SerLysAsnThrSerLysThrProGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:399:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:399:                                      GluGlnGlnProSerLeuLysProSerSerGlySerProGlyAsnPro                               151015                                                                         SerLysSerThrSerLysThrThrGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:400:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:400:                                      AspGlnGlnProGlyLeuLysProSerSerGlySerProGlyAsnPro                               151015                                                                         SerLysAsnThrSerGluThrThrGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:401:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:401:                                      AspGlnGlnProGlyLeuLysProSerSerGlySerProGlyAsnPro                               151015                                                                         SerLysAsnThrSerGluThrThrGlxThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:402:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:402:                                      GluGlnGlnProSerLeuLysProSerSerGlySerProGlyAsnPro                               151015                                                                         SerLysSerThrSerLysThrSerGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:403:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:403:                                      GluGlnGlnProSerLeuLysProSerSerGlySerProGlyAsnPro                               151015                                                                         SerLysSerThrSerArgThrThrGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:404:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:404:                                      GluGlnGlnProSerLeuLysProSerSerGlySerProGlyAsnPro                               151015                                                                         SerLysSerThrSerLysThrAlaGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:405:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:405:                                      AspGlnGlnProAspLeuLysProSerSerGlyPheProGlyAsnPro                               151015                                                                         SerLysSerThrSerLysThrThrGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:406:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:406:                                      GluGlnGlnProSerLeuLysProSerSerGlySerProGlyLysPro                               151015                                                                         SerLysSerThrSerLysThrAsnGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:407:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:407:                                      GluGlnGlnProSerLeuLysProSerSerGlySerProGlyAsnPro                               151015                                                                         SerLysSerThrPheLysThrSerGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:408:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:408:                                      GluGlnGlnProSerLeuLysProSerSerGlySerProGlyAsnPro                               151015                                                                         SerLysSerThrSerThrThrSerGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:409:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:409:                                      GluGlnGlnLeuSerLeuLysProSerSerGlySerProGlyAsnPro                               151015                                                                         SerLysSerThrSerLysThrThrGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:410:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:410:                                      GlnGlnGlnProGlyLeuLysProSerPheGlyProProGlyLysPro                               151015                                                                         SerGlnSerThrSerLysThrThrGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:411:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:411:                                      GlnGlnLysProGlyLeuAlaProSerSerGlySerProGlyLysSer                               151015                                                                         ThrLysSerAsnSerLysGlnThrAspThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:412:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:412:                                      GlnGlnLysProGlyLeuAlaProSerSerGlySerProGlyLysSer                               151015                                                                         AlaLysSerAsnSerLysGlnThrAspThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:413:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:413:                                      GlnGlnLysProGlyLeuAlaProSerSerGlySerProGlyLysSer                               151015                                                                         AlaMetSerAsnSerLysGlnThrAspThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:414:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:414:                                      GlnGlnLysProGlyLeuAlaProSerSerGlySerProGlyLysSer                               151015                                                                         AlaIleSerAsnSerLysGlnThrAspThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:415:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:415:                                      GlnGlnLysProGlyLeuGlnProSerSerGlySerProGlyLysAla                               151015                                                                         AlaIleSerAsnSerLysGlnSerAsnThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:416:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:416:                                      GlnGlnLysProGlyLeuGlnProSerSerGlySerProGlyLysAla                               151015                                                                         AlaIleSerAsnSerLysGlnAlaAsnThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:417:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:417:                                      GlnGlnLysProValLeuAlaProSerSerGlySerProGlyLysSer                               151015                                                                         AlaMetSerAsnSerLysGlnIleAspThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:418:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:418:                                      GlnGlnLysProSerLeuGlnProSerSerAspSerProGlyLysAla                               151015                                                                         AlaMetSerAsnSerLysGlnAlaAspThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:419:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:419:                                      GluArgValGlyAspLeuGluProGlyArgGlyIleProGlyLysAla                               151015                                                                         ProLysGlyAspSerLysLysIleGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:420:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:420:                                      GluArgValGlyAspLeuGluProGluArgGlyIleProGlyLysAla                               151015                                                                         ProLysGlyAspSerLysLysIleGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:421:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:421:                                      GluGlnValGlyGlyLeuLysProGlyArgGlyThrProGlyLysAla                               151015                                                                         ProLysGlyAspSerLysLysThrGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:422:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:422:                                      GluGlnValGlyGlyLeuGlnProGlyLysGlyThrSerGlyLysAla                               151015                                                                         SerLysGlyAspSerLysLysThrGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:423:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:423:                                      GluGlnLeuGlyGlyLeuGlnProGlyArgGlyThrProGlyLysAsx                               151015                                                                         SerLysGlyAspSerLysArgAlaGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:424:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:424:                                      GluGlnLeuGlyGlyLeuGlnProGlyArgGlyThrProGlyLysAsp                               151015                                                                         SerLysGlyAsnSerLysArgAlaGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:425:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:425:                                      GluGlnLeuGlyGlyLeuGlnProGlyArgGlyThrProGlyLysAsp                               151015                                                                         SerArgGlyAsnSerLysArgAlaGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:426:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:426:                                      GluGlnValGlyGlyLeuGlnProGlyArgGlyThrProGlyLysAsp                               151015                                                                         SerLysGlyAsnSerLysArgAlaGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:427:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:427:                                      GluGlnValGlyGlyLeuGlnProGlyArgGlyThrProGlyLysAsp                               151015                                                                         SerLysGlyAsnAlaLysArgAlaGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:428:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:428:                                      GluGlnValGlyGlyLeuGlnProGlyArgGlyThrProGlyLysAsp                               151015                                                                         SerLysGlyAspSerArgArgAlaGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:429:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:429:                                      GluGlnValGlyGlyLeuGlnProGlyArgGlyThrProGlyLysAsp                               151015                                                                         SerLysGlyAsnSerArgArgAlaGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:430:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:430:                                      GlnGlnValGlyGlyLeuGluProGlyArgGlyThrProGlyLysAsp                               151015                                                                         SerLysGlyAsxSerLysArgAlaGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:431:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:431:                                      GluGlnLeuGlyAspLeuGlnProGlyArgGlyThrProGlyLysAla                               151015                                                                         SerLysGlyAsnSerLysArgAlaGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:432:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:432:                                      GluGlnValGlyGlyLeuGlnProGlyArgGlyThrThrGlyLysAsp                               151015                                                                         SerLysGlyAspSerLysArgAlaGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:433:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:433:                                      GlnGlnValGlyGlyValGlnProGlyArgGlyThrProGlyLysAsp                               151015                                                                         SerLysGlyAsnSerLysArgAlaGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:434:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:434:                                      GlnGlnValGlyGlyValGlnProGlyArgGlyIleProGlyLysAsp                               151015                                                                         SerLysGlyAsnSerLysArgProGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:435:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:435:                                      GluGlnValGlyGlyValGlnProGlyArgGlyIleProGlyLysAsp                               151015                                                                         SerLysGlyAspSerLysArgProGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:436:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:436:                                      GlnGlnValGlyGlyValGlnProGlyArgGlyThrProGlyLysAsp                               151015                                                                         SerAsnGlyAspSerLysArgProGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:437:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:437:                                      GlnLysValGlyGlyValGlnProGlyArgGlyThrProGlyLysAsp                               151015                                                                         SerLysGlyAsnSerLysArgThrGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:438:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:438:                                      GlnGluValGlyGlyValGlxProGlyArgGlyThrProGlyLysAsx                               151015                                                                         SerLysGlyAsxSerLysArgAlaGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:439:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:439:                                      GluGlnLeuGlyGlyLeuGlnProGlyArgGlyThrProGlyLysAsp                               151015                                                                         SerAsnGlyAspSerLysGlnAlaGlxThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:440:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:440:                                      GluGlnLeuGlyGlyLeuGlnProGlyArgGlySerProGlyLysAsp                               151015                                                                         ThrAsnGlyAspSerLysGluAlaGlxThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:441:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:441:                                      AlaGlnLeuGlyGlyLeuGlnProGlyArgGlyThrProGlyLysAsp                               151015                                                                         SerAsnGlyAspSerLysGlnAlaGlxSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:442:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:442:                                      GluGlnLeuGlyGlyLeuGlnProGlyArgGlyThrProGlyLysVal                               151015                                                                         SerGlnGlyAspSerLysGlnAlaGlxThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:443:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:443:                                      GluGlnValGlyGlyLeuGlnProGlyArgGlyThrProGlyLysVal                               151015                                                                         SerGlnGlyAspSerLysGluProGlxThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:444:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:444:                                      GluGlnLeuGlyGlyLeuGlnProGluArgGlyThrProGlyLysGlu                               151015                                                                         SerLysGlyAsnSerMetArgAlaGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:445:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:445:                                      GluGlnValGlyAspLeuGlnProGlyArgGlyAsxProGlyLysAsp                               151015                                                                         SerLysGlyAsnAlaLysArgValGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:446:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:446:                                      GluGlnValGlyAspLeuGlnProGlyArgGlyAsnProGlyLysAsp                               151015                                                                         SerLysGlyAsnAlaGlnArgProGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:447:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:447:                                      GlnGlnValGlyGlyValGlnProGlyArgGlyThrLeuGlyLysAsp                               151015                                                                         SerLysGlyAsnSerLysArgAlaGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:448:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:448:                                      GlnGlxValGlyGlyAlaGlxProGlyArgGlySerProGlyLysAla                               151015                                                                         SerLysGlyAsxSerLysArgAlaGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:449:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:449:                                      GlnGlnValGlyGlyLeuLysProGlyArgGlySerProGlyLysAsp                               151015                                                                         SerLysGlyAsnAlaGlnArgThrGlxThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:450:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:450:                                      AspGlnValGlyGlyLeuLysProGlyArgGlyThrProGlyLysAsn                               151015                                                                         SerAsnGlyAspSerLysThrProGlxThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:451:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:451:                                      GluGlnLeuGlyGlyLeuGlnProGlyArgGlyThrSerArgGluAsp                               151015                                                                         SerLysGlyAsnSerLysArgAlaGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:452:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:452:                                      GluGlnValGlyAlaLeuGlnProGlyArgGlyThrProGlyLysAsp                               151015                                                                         SerGlnAlaAspSerLysGluAlaGlxThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:453:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 22 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:453:                                      GluGlnLeuGlyGlyLeuGlnProGlyArgGlyThrProGlyLysVal                               151015                                                                         GluGlySerValGluThr                                                             20                                                                             (2) INFORMATION FOR SEQ ID NO:454:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:454:                                      GluGlnValGlyAlaPheGlnProGlyArgGlyAsnSerGlyLysAla                               151015                                                                         SerLysGlyAspSerLysArgProAspThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:455:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:455:                                      GluGlnValGlyAlaPheGlnProGlyLysGlyAsnSerGlyLysAla                               151015                                                                         SerLysGlyAspSerLysArgProAspThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:456:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:456:                                      GluGlnValGlyAlaPheGlnProGlyLysGlyAsnSerGlyLysAla                               151015                                                                         SerLysGlyAspSerAsnArgProAspThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:457:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:457:                                      GlnGlnValGlyGlyValGlnAlaGlyArgAlaAsnProGlyLysAsp                               151015                                                                         SerArgGlyIleSerLysArgThrGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:458:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:458:                                      GlnGlnValAlaGluValLysProGlyLysGlyThrProGlyGlnGln                               151015                                                                         LysGlnGlyGluSerThrArgSerGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:459:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:459:                                      GlnGlnValAlaGluValLysProGlyLysGlyThrProGlyGlnGln                               151015                                                                         LysGlnGlyThrSerThrArgSerGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:460:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:460:                                      GlnGlnValAlaGluValLysProGlyLysGlyThrProGlyGlnGln                               151015                                                                         LysGlnGlyThrSerAlaArgSerGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:461:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:461:                                      GlnGlnValAlaGluValLysProGlyLysGlyThrProGlyGlnGln                               151015                                                                         LysGlnGlyThrSerIleArgSerAspThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:462:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:462:                                      GlnGlnValAlaGluValLysProGlyLysGlyThrProGlyGlnGlu                               151015                                                                         LysGlnGlyThrSerIleArgSerAspThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:463:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:463:                                      GlnGlnValAlaGluValLysProGlyLysGlyThrProGlyGlnGln                               151015                                                                         AsnGlnGlyThrSerThrArgSerAspThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:464:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:464:                                      GlnGlnValGlyGluValLysProGlyArgGlyThrProGlyGlnGln                               151015                                                                         LysGlnAspThrSerThrArgSerAspThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:465:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:465:                                      GlnGlnValAlaGluValLysProGlyArgGlyThrProGlyHisPro                               151015                                                                         ArgGlnGlyAlaSerPheArgSerAspSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:466:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:466:                                      GlnGlnValSerGluLeuLysProGlyLysGlyThrProGlyGlnGln                               151015                                                                         GlyThrGlyThrSerValLysAlaGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:467:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:467:                                      GluGlnValAlaGluValLysProGlyLysGlySerProGlyLysPro                               151015                                                                         SerGlnGlyLysSerIleLysAlaSerThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:468:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:468:                                      GluGlnValAlaGluValLysProGlyArgGlySerProGlyLysPro                               151015                                                                         SerGlnGlyLysSerIleLysAlaSerThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:469:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:469:                                      GlnGlnValAlaGluValLysProGlyArgGlyAspProGlyArgPro                               151015                                                                         ArgGlnAlaSerSerThrIleSerAlaThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:470:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:470:                                      GluGlnValAlaGluValProGlnGlyLysGlyArgProGlyLysSer                               151015                                                                         LeuGlnGlyLysSerLeuLysAlaSerThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:471:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:471:                                      GlnGlnMetAlaGluValLysProGlyArgGlyThrProGlyLysPro                               151015                                                                         GlyValValProSerPhePheSerGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:472:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:472:                                      GlnGlnValAlaGluValLysProGlyArgGlyThrProGlyArgTyr                               151015                                                                         IleTrpGluProSerPhePheAsnGluGly                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:473:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:473:                                      GlnGlnGlnAlaGlyLeuLysProSerSerGlySerProGlyLysPro                               151015                                                                         SerLysSerThrSerLysThrAlaAlaThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:474:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:474:                                      GlnGlnGlnProGlyLeuLysProSerSerGlySerProGlyLysPro                               151015                                                                         SerLysSerThrSerLysThrAlaAlaThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:475:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:475:                                      GlnGlnGlnProGlyLeuLysProSerSerGlySerProGlyLysPro                               151015                                                                         SerLysSerThrSerAsnThrAlaAlaThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:476:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:476:                                      GlnGlnGlnProGlyLeuLysProSerSerGlySerAlaGlyLysPro                               151015                                                                         SerLysSerThrSerLysThrAlaAlaThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:477:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:477:                                      ArgGlnGlnProGlyLeuLysProSerSerGlyProProGlyLysPro                               151015                                                                         SerArgGlyThrSerArgSerAlaAlaThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:478:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:478:                                      GlnGlnGlnAlaGlyLeuLysProSerSerGlySerProGlyArgThr                               151015                                                                         SerLysSerThrSerLysThrAlaAlaThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:479:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:479:                                      GlnGlnGluProGlyLeuArgProSerSerGlyThrProGlyArgThr                               151015                                                                         ProArgSerThrSerLysThrAlaAlaThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:480:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:480:                                      XaaGlnGluProGlyLeuArgProSerSerGlySerProGlyArgThr                               151015                                                                         ProArgSerThrSerLysThrAlaAlaThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:481:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:481:                                      GlnGlnGlnProGlyLeuLysProSerSerGlySerProSerArgVal                               151015                                                                         SerLysSerThrSerLysThrProGluThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:482:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:482:                                      GlnHisGlnAlaGlyLeuLysArgSerSerGlyProProGlyLysPro                               151015                                                                         SerThrSerThrSerLysThrAlaAlaThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:483:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:483:                                      GlxGlnGluSerGlyLeuLysProThrSerGlySerProGlyLysPro                               151015                                                                         SerLysSerArgSerLysAlaAlaAspAla                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:484:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:484:                                      GlnThrLysProThrLeuLysProThrThrGlySerProGlyArgPro                               151015                                                                         SerLysSerThrSerLysAspProValThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:485:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:485:                                      GlnThrLysProThrLeuLysProThrThrGlySerProGlyLysPro                               151015                                                                         SerArgSerThrSerArgAspProValSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:486:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:486:                                      GluThrArgProAlaLeuLysProThrThrGlySerProGlyLysThr                               151015                                                                         SerLysThrThrSerLysAspProValThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:487:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:487:                                      GlnAsnArgProAlaLeuLysAlaThrThrGlySerProGlyLysThr                               151015                                                                         SerGluThrThrSerLysAspProAlaThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:488:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:488:                                      GlnThrThrProAlaLeuLysProLysThrGlySerProGlyLysThr                               151015                                                                         SerArgThrAspSerLysAsnProValThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:489:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:489:                                      GlnThrArgProAlaLeuArgProThrThrGlySerProGlyGluAla                               151015                                                                         SerGluThrThrSerLysGlyProGlyThr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:490:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:490:                                      GlnThrArgProAlaLeuLysProThrThrGlySerProGlyLysThr                               151015                                                                         SerGluThrThrSerArgAspThrAlaTyr                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:491:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 26 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:491:                                      LeuGluGlyValGlnLeuTrpGlyGlyArgGlyIleSerArgLysTyr                               151015                                                                         AlaLysGlyAsnGlyLysArgGluAspSer                                                 2025                                                                           (2) INFORMATION FOR SEQ ID NO:492:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 10 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:492:                                      TyrAsnAsnProGlyAsnGlyTyrIleAla                                                 1510                                                                           (2) INFORMATION FOR SEQ ID NO:493:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 10 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:493:                                      TyrIleAsnProGlyLysGlyTyrLeuSer                                                 1510                                                                           (2) INFORMATION FOR SEQ ID NO:494:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 11 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:494:                                      ArgAlaSerGlnGluIleSerGlyTyrLeuSer                                              1510                                                                           (2) INFORMATION FOR SEQ ID NO:495:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 11 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:495:                                      ArgAlaSerGlyAsnIleHisAsnTyrLeuAla                                              1510                                                                           (2) INFORMATION FOR SEQ ID NO:496:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 11 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:496:                                      ArgAlaSerGlnAspIleAsnAsnPheLeuAsn                                              1510                                                                           (2) INFORMATION FOR SEQ ID NO:497:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 11 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:497:                                      ArgAlaSerGlnSerIleGlyAsnAsnLeuHis                                              1510                                                                           (2) INFORMATION FOR SEQ ID NO:498:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 7 amino acids                                                      (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:498:                                      AlaAlaSerThrLeuAspSer                                                          15                                                                             (2) INFORMATION FOR SEQ ID NO:499:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 7 amino acids                                                      (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:499:                                      TyrThrThrThrLeuAlaAsp                                                          15                                                                             (2) INFORMATION FOR SEQ ID NO:500:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 7 amino acids                                                      (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:500:                                      PheThrSerArgSerGlnSer                                                          15                                                                             (2) INFORMATION FOR SEQ ID NO:501:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 7 amino acids                                                      (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:501:                                      LysAlaSerSerLeuGluSer                                                          15                                                                             (2) INFORMATION FOR SEQ ID NO:502:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 9 amino acids                                                      (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:502:                                      LeuGlnTyrLeuSerTyrProLeuThr                                                    15                                                                             (2) INFORMATION FOR SEQ ID NO:503:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 9 amino acids                                                      (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:503:                                      GlnHisPheTrpSerThrProArgThr                                                    15                                                                             (2) INFORMATION FOR SEQ ID NO:504:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 9 amino acids                                                      (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:504:                                      GlnGlnGlyAsnAlaLeuProArgThr                                                    15                                                                             (2) INFORMATION FOR SEQ ID NO:505:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 7 amino acids                                                      (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:505:                                      GlnGlnTyrAsnSerTyrSer                                                          15                                                                             (2) INFORMATION FOR SEQ ID NO:506:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 5 amino acids                                                      (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:506:                                      ThrPheGlyIleThr                                                                15                                                                             (2) INFORMATION FOR SEQ ID NO:507:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 5 amino acids                                                      (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:507:                                      GlyTyrGlyValAsn                                                                15                                                                             (2) INFORMATION FOR SEQ ID NO:508:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 5 amino acids                                                      (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:508:                                      SerAsnGlyIleAsn                                                                15                                                                             (2) INFORMATION FOR SEQ ID NO:509:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 5 amino acids                                                      (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:509:                                      AspTyrAlaMetHis                                                                15                                                                             (2) INFORMATION FOR SEQ ID NO:510:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 10 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:510:                                      GluIlePheProGlyAsnSerLysThrTyr                                                 1510                                                                           (2) INFORMATION FOR SEQ ID NO:511:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 9 amino acids                                                      (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:511:                                      MetIleTrpGlyAspGlyAsnThrAsp                                                    15                                                                             (2) INFORMATION FOR SEQ ID NO:512:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 10 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:512:                                      TyrAsnAsnProGlyAsnGlyTyrIleAla                                                 1510                                                                           (2) INFORMATION FOR SEQ ID NO:513:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 9 amino acids                                                      (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:513:                                      IleSerTrpAspSerSerSerIleGly                                                    15                                                                             (2) INFORMATION FOR SEQ ID NO:514:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 5 amino acids                                                      (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:514:                                      ArgGluIleArgTyr                                                                15                                                                             (2) INFORMATION FOR SEQ ID NO:515:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 8 amino acids                                                      (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:515:                                      GluArgAspTyrArgLeuAspTyr                                                       15                                                                             (2) INFORMATION FOR SEQ ID NO:516:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 12 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:516:                                      SerGluTyrTyrGlyGlySerTyrLysPheAspTyr                                           1510                                                                           (2) INFORMATION FOR SEQ ID NO:517:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 17 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:517:                                      GlyArgAspTyrTyrAspSerGlyGlyTyrPheThrValAlaPheAsp                               151015                                                                         Ile                                                                            (2) INFORMATION FOR SEQ ID NO:518:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 11 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:518:                                      ArgAlaSerGlnSerIleSerArgTrpLeuAla                                              1510                                                                           (2) INFORMATION FOR SEQ ID NO:519:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 7 amino acids                                                      (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:519:                                      GluAlaSerAsnAspLeuAla                                                          15                                                                             (2) INFORMATION FOR SEQ ID NO:520:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 5 amino acids                                                      (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:520:                                      AspPheTyrMetGlu                                                                15                                                                             (2) INFORMATION FOR SEQ ID NO:521:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 9 amino acids                                                      (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:521:                                      IleIleTrpAspAspGlySerAspGln                                                    15                                                                             (2) INFORMATION FOR SEQ ID NO:522:                                             (i) SEQUENCE CHARACTERISTICS:                                                  (A) LENGTH: 11 amino acids                                                     (B) TYPE: amino acid                                                           (C) TOPOLOGY: linear                                                           (ii) MOLECULE TYPE: peptide                                                    (xi) SEQUENCE DESCRIPTION: SEQ ID NO:522:                                      GlnAlaSerGlnSerIleIleLysTyrLeuAsn                                              1510                                                                           __________________________________________________________________________ 

What is claimed is:
 1. A method for producing a humanized rodent antibody or fragment thereof by resurfacing, said method consisting essentially of:(a) generating position alignments from relative accessibility distributions from x-ray crystallographic structures of a pool of rodent and human antibody heavy and light chain variable regions to give a set of heavy and light chain variable region framework surface exposed positions wherein the alignment positions for all rodent and human variable regions are at least about 98% identical; (b) defining for a rodent antibody or fragment thereof a set of heavy and light chain variable region framework surface exposed amino acid residues using said set of heavy and light chain variable region framework surface exposed positions generated in said step (a); (c) identifying from human antibody amino acid sequences a set of heavy and light chain variable region framework surface exposed amino acid residues that is most closely identical to said set of rodent surface exposed amino acid residues defined in said step (b); (d) substituting, in the variable region framework amino acid sequence of said rodent antibody or fragment thereof, said set of heavy and light chain variable region framework surface exposed amino acid residues defined in said step (b) with said set of heavy and light chain variable region framework surface exposed amino acid residues identified in said step (c); (e) constructing three-dimensional models of said variable region of said rodent antibody or fragment thereof and of said variable region of said rodent antibody or fragment thereof resulting from the substituting specified in said step (d); (f) comparing said three-dimensional models constructed in said step (e), and identifying any amino acid residues from said sets identified in said steps (b) or (c), that are within 5 Ångstroms of any atom of any residue of the complementarity determining regions of said rodent antibody or fragment thereof; (g) changing any residues identified in said step (f) from the human to the original rodent amino acid residue to thereby define a humanizing set of surface exposed amino acid residues; (h) replacing the set of rodent antibody variable region framework surface exposed amino acid residues defined in said step (b) with the humanizing set of variable region framework surface exposed amino acid residues defined in said step (g); and (i) producing said humanized rodent antibody or fragment thereof having binding specificity.
 2. The method of claim 1, wherein said rodent antibody is an antibody fragment.
 3. The method of claim 2, wherein said rodent antibody fragment is a single chain antibody, a F_(V) fragment, a Fab fragment, a F(ab')₂ fragment or a Fab' fragment.
 4. The method of claim 1 or 2, wherein said step (c) identifies a set of naturally paired heavy and light chain surface exposed amino acid residues that is most closely identical to said set of surface exposed amino acid residues defined in said step (b).
 5. The method of claim 1 or 2, wherein the set of heavy and light chain surface exposed positions in step (a) is identical in at least 98% of said sequence alignment positions.
 6. The method of claim 1 or 2, wherein said surface exposed amino acid residues are those residues whose solvent accessibility is above 30%.
 7. The method of claim 1 or 2, wherein the rodent antibody or fragment thereof is a murine antibody.
 8. The method of claim 1 or 2, wherein the rodent antibody or fragment thereof is murine antibody anti-N901.
 9. The method of claim 1 or 2, wherein said set of framework positions of surface exposed amino acid residues is defined by the set shown in Table 1 and the alignments set forth in FIGS. 3A and 3B:

                  TABLE 1                                                          ______________________________________                                         Posi-                                                                          tion  Human   Mouse                                                            ______________________________________                                         Light chain                                                                    1     D 51 E 34 A 5 S 5 D 76 Q 9 E 6                                           3     V 38 Q 24 S 24 Y 6                                                                               V 63 Q 22 L 5                                          5     T 61 L 37         T 87                                                   9     9 26 S 26 G 17 A 14 L 7                                                                          S 36 A 29 L 17 P 5                                     15    P 62 V 25 L 12    L 47 P 30 V 8 A 7                                      18    R 57 S 18 T 13 P 6                                                                               R 38 K 22 S 13 Q 12 T 9                                46    P 94              P 82 S 9                                               47    G 89              G 71 D 18                                              51    K 43 R 31         K 70 Q 13 R 8 T 5                                      63    G 91              G 98                                                   66    D 43 S 25 A 9     D 38 A 26 S 26                                         73    S 96              S 90 I 5                                               86    P 44 A 27 S 17 T 8                                                                               A 50 P 11 T 8 E 7 Q 6                                  87    E 71 D 11 G 7     E 91 D 6                                               111   K 74 R 12 N 6     K 93                                                   115   K 54 L 40         K 87 L 5                                               116   R 60 G 33 S 5     R 89 G 9                                               117   Q 50 T 37 E 6 P 6 A 74 Q 14 P 5 R 5                                      Heavy chain                                                                    118   E 47 Q 46         E 59 Q 29 D 10                                         120   Q 83 T 7          Q 68 K 26                                              122   V 59 L 15 Q 13    Q 57 V 27 L 5 K 5                                      126   G 54 A 23 P 18    G 36 9 30 A 29                                         127   G 53 E 22 A 14 D 7                                                                               E 45 G 43 S 6                                          128   L 61 V 31 F 7     L 96                                                   130   K 46 Q 41 E 5     K 52 Q 27 R 17                                         131   P 95              P 91 A 5                                               132   G 74 S 16 T 7     G 82 S 17                                              136   R 53 K 23 S 17 T 7                                                                               K 66 S 17 R 13                                         143   G 96              G 98                                                   145   T 46 S 32 N 9 I 7 T 63 S 19 N 7 A 5 D 5                                  160   P 84 S 10         P 89 H 7                                               161   G 93              G 71 E 24                                              162   K 76 Q 10 R 8     K 50 Q 30 N 10 H 5                                     183   D 26 P 25 A 17 Q 10 T 7                                                                          E 31 P 22 D 17 A 12 Q 11                               184   S 70 K 9 P 8      K 42 S 37 T 6                                          186   K 53 Q 22 R 7 N 5 K 83 Q 7                                               187   G 66 S 21 T 5     G 62 S 18 D 10                                         195   T 30 D 26 N 19 K 7                                                                               T 36 K 30 N 26 D 6                                     196   S 91              S 76 A 16                                              197   K 65 I 8 T 8 R 5  S 46 K 34 Q 11                                         208   R 46 T 18 K 17 D 6                                                                               T 55 R 26 K 8                                          209   A 50 P 21 S 13 T 8                                                                               S 67 A 14 T 11                                         210   E 46 A 18 D 13 S 9 Z 8 V 5                                                                       E 88 D 7                                               212   T 91              T 53 S 43                                              222   G 17 D 11 P 10 Y 9 V N 8                                                                         D 67 A
 18.                                             ______________________________________                                    


10. A resurfaced rodent antibody or fragment thereof made by the method of claim
 1. 11. The resurfaced antibody or fragment thereof of claim 10, wherein said rodent antibody is an antibody fragment.
 12. The resurfaced antibody or fragment thereof of claim 10, wherein said rodent antibody fragment is a single chain antibody, a F_(V) fragment, a Fab fragment, a F(ab')₂ fragment or a Fab' fragment.
 13. The resurfaced antibody or fragment thereof of claim 10 or 11, wherein said surface exposed amino acid residues are those residues whose solvent accessibility is above 30%.
 14. The resurfaced antibody or fragment thereof of claim 10, or 11, wherein the rodent antibody or fragment thereof is a murine antibody.
 15. The resurfaced antibody or fragment thereof of claim 10 or 11, wherein the rodent antibody or fragment thereof to be humanized is murine antibody anti-N901.
 16. The resurfaced antibody or fragment thereof of claim 10 or 11, wherein said set of framework positions of surface exposed amino acid residues is defined by the set shown in Table 1 and the alignments set forth in FIGS. 3A and 3B:

                  TABLE 1                                                          ______________________________________                                         Posi-                                                                          tion  Human   Mouse                                                            ______________________________________                                         Light chain                                                                    1     D 51 E 34 A 5 S 5 D 76 Q 9 E 6                                           3     V 38 Q 24 S 24 Y 6                                                                               V 63 Q 22 L 5                                          5     T 61 L 37         T 87                                                   9     9 26 S 26 G 17 A 14 L 7                                                                          S 36 A 29 L 17 P 5                                     15    P 62 V 25 L 12    L 47 P 30 V 8 A 7                                      18    R 57 S 18 T 13 P 6                                                                               R 38 K 22 S 13 Q 12 T 9                                46    P 94              P 82 S 9                                               47    G 89              G 71 D 18                                              51    K 43 R 31         K 70 Q 13 R 8 T 5                                      63    G 91              G 98                                                   66    D 43 S 25 A 9     D 38 A 26 S 26                                         73    S 96              S 90 I 5                                               86    P 44 A 27 S 17 T 8                                                                               A 50 P 11 T 8 E 7 Q 6                                  87    E 71 D 11 G 7     E 91 D 6                                               111   K 74 R 12 N 6     K 93                                                   115   K 54 L 40         K 87 L 5                                               116   R 60 G 33 S 5     R 89 G 9                                               117   Q 50 T 37 E 6 P 6 A 74 Q 14 P 5 R 5                                      Heavy chain                                                                    118   E 47 Q 46         E 59 Q 29 D 10                                         120   Q 83 T 7          Q 68 K 26                                              122   V 59 L 15 Q 13    Q 57 V 27 L 5 K 5                                      126   G 54 A 23 P 18    G 36 9 30 A 29                                         127   G 53 E 22 A 14 D 7                                                                               E 45 G 43 S 6                                          128   L 61 V 31 F 7     L 96                                                   130   K 46 Q 41 E 5     K 52 Q 27 R 17                                         131   P 95              P 91 A 5                                               132   G 74 S 16 T 7     G 82 S 17                                              136   R 53 K 23 S 17 T 7                                                                               K 66 S 17 R 13                                         143   G 96              G 98                                                   145   T 46 S 32 N 9 I 7 T 63 S 19 N 7 A 5 D 5                                  160   P 84 S 10         P 89 H 7                                               161   G 93              G 71 E 24                                              162   K 76 Q 10 R 8     K 50 Q 30 N 10 H 5                                     183   D 26 P 25 A 17 Q 10 T 7                                                                          E 31 P 22 D 17 A 12 Q 11                               184   S 70 K 9 P 8      K 42 S 37 T 6                                          186   K 53 Q 22 R 7 N 5 K 83 Q 7                                               187   G 66 S 21 T 5     G 62 S 18 D 10                                         195   T 30 D 26 N 19 K 7                                                                               T 36 K 30 N 26 D 6                                     196   S 91              S 76 A 16                                              197   K 65 I 8 T 8 R 5  S 46 K 34 Q 11                                         208   R 46 T 18 K 17 D 6                                                                               T 55 R 26 K 8                                          209   A 50 P 21 S 13 T 8                                                                               S 67 A 14 T 11                                         210   E 46 A 18 D 13 S 9 Z 8 V 5                                                                       E 88 D 7                                               212   T 91              T 53 S 43                                              222   G 17 D 11 P 10 Y 9 V N 8                                                                         D 67 A
 18.                                             ______________________________________                                     